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The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects

BACKGROUND: There is an increasing demand for serum 25‐OH VitD testing globally, and this has led to the greater use of automated immunoassays. These may be more prone to non‐specific interference, that is thought to be related to pre‐analytical stability of biological samples. We have investigated...

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Autores principales: Borai, Anwar, Khalil, Haitham, Alghamdi, Basma, Alhamdi, Raghad, Ali, Najwa, Bahijri, Suhad, Ferns, Gordon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031638/
https://www.ncbi.nlm.nih.gov/pubmed/31587378
http://dx.doi.org/10.1002/jcla.23037
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author Borai, Anwar
Khalil, Haitham
Alghamdi, Basma
Alhamdi, Raghad
Ali, Najwa
Bahijri, Suhad
Ferns, Gordon
author_facet Borai, Anwar
Khalil, Haitham
Alghamdi, Basma
Alhamdi, Raghad
Ali, Najwa
Bahijri, Suhad
Ferns, Gordon
author_sort Borai, Anwar
collection PubMed
description BACKGROUND: There is an increasing demand for serum 25‐OH VitD testing globally, and this has led to the greater use of automated immunoassays. These may be more prone to non‐specific interference, that is thought to be related to pre‐analytical stability of biological samples. We have investigated the changes in serum 25‐OH VitD concentrations that are caused by storage and mixing conditions, and if such changes are statistical, or clinically important. METHODS: Blood samples were collected into plain tubes from 31 healthy donors. After separation, serum samples were stored at −20°C and analysis was carried out with and without mixing (vortexing) at different time intervals of days (0, 1, 2, 3, 4, 5, 15, and 30). All samples were analyzed using a chemiluminescent immunoassay. RESULTS: Mean serum 25‐OH VitD concentrations for subsequent days of storage compared with day 0 showed a significant time effect (P < .05) except for the samples on day 1 (P = .654) in non‐vortexed samples and day 2 (P = .087), 5 (P = .118) and 30 (P = .118) in vortexed samples. Comparing values for vortexed and non‐vortexed samples on the same day, serum 25‐OH VitD showed a significant difference on days 1 (P = .003), 4 (P = .037), 5 (P = .002), and 30 (P = .025). However, the maximum change value was 8.85% which was less than the known total allowable error (TEa) and reference change value (RCV) for serum 25‐OH VitD. CONCLUSION: 25‐OH VitD is pre‐analytically stable after long‐term sample storage at −20°C and can be analyzed without vortexing. This may be beneficial for both research and diagnostic laboratories.
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spelling pubmed-70316382020-02-27 The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects Borai, Anwar Khalil, Haitham Alghamdi, Basma Alhamdi, Raghad Ali, Najwa Bahijri, Suhad Ferns, Gordon J Clin Lab Anal Research Articles BACKGROUND: There is an increasing demand for serum 25‐OH VitD testing globally, and this has led to the greater use of automated immunoassays. These may be more prone to non‐specific interference, that is thought to be related to pre‐analytical stability of biological samples. We have investigated the changes in serum 25‐OH VitD concentrations that are caused by storage and mixing conditions, and if such changes are statistical, or clinically important. METHODS: Blood samples were collected into plain tubes from 31 healthy donors. After separation, serum samples were stored at −20°C and analysis was carried out with and without mixing (vortexing) at different time intervals of days (0, 1, 2, 3, 4, 5, 15, and 30). All samples were analyzed using a chemiluminescent immunoassay. RESULTS: Mean serum 25‐OH VitD concentrations for subsequent days of storage compared with day 0 showed a significant time effect (P < .05) except for the samples on day 1 (P = .654) in non‐vortexed samples and day 2 (P = .087), 5 (P = .118) and 30 (P = .118) in vortexed samples. Comparing values for vortexed and non‐vortexed samples on the same day, serum 25‐OH VitD showed a significant difference on days 1 (P = .003), 4 (P = .037), 5 (P = .002), and 30 (P = .025). However, the maximum change value was 8.85% which was less than the known total allowable error (TEa) and reference change value (RCV) for serum 25‐OH VitD. CONCLUSION: 25‐OH VitD is pre‐analytically stable after long‐term sample storage at −20°C and can be analyzed without vortexing. This may be beneficial for both research and diagnostic laboratories. John Wiley and Sons Inc. 2019-10-06 /pmc/articles/PMC7031638/ /pubmed/31587378 http://dx.doi.org/10.1002/jcla.23037 Text en © 2019 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Borai, Anwar
Khalil, Haitham
Alghamdi, Basma
Alhamdi, Raghad
Ali, Najwa
Bahijri, Suhad
Ferns, Gordon
The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects
title The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects
title_full The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects
title_fullStr The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects
title_full_unstemmed The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects
title_short The pre‐analytical stability of 25‐hydroxyvitamin D: Storage and mixing effects
title_sort pre‐analytical stability of 25‐hydroxyvitamin d: storage and mixing effects
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031638/
https://www.ncbi.nlm.nih.gov/pubmed/31587378
http://dx.doi.org/10.1002/jcla.23037
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