Cargando…

Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS

[Image: see text] Detailed characterization of complex biological surfaces by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) requires instrumentation that is capable of high mass resolving power, mass accuracy, and dynamic range. Fourier transform ion cyclotron r...

Descripción completa

Detalles Bibliográficos
Autores principales: Bowman, Andrew P., Blakney, Greg T., Hendrickson, Christopher L., Ellis, Shane R., Heeren, Ron M. A., Smith, Donald F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031845/
https://www.ncbi.nlm.nih.gov/pubmed/31955581
http://dx.doi.org/10.1021/acs.analchem.9b04768
_version_ 1783499452458205184
author Bowman, Andrew P.
Blakney, Greg T.
Hendrickson, Christopher L.
Ellis, Shane R.
Heeren, Ron M. A.
Smith, Donald F.
author_facet Bowman, Andrew P.
Blakney, Greg T.
Hendrickson, Christopher L.
Ellis, Shane R.
Heeren, Ron M. A.
Smith, Donald F.
author_sort Bowman, Andrew P.
collection PubMed
description [Image: see text] Detailed characterization of complex biological surfaces by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) requires instrumentation that is capable of high mass resolving power, mass accuracy, and dynamic range. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) offers the highest mass spectral performance for MALDI MSI experiments, and often reveals molecular features that are unresolved on lower performance instrumentation. Higher magnetic field strength improves all performance characteristics of FT-ICR; mass resolving power improves linearly, while mass accuracy and dynamic range improve quadratically with magnetic field strength. Here, MALDI MSI at 21T is demonstrated for the first time: mass resolving power in excess of 1 600 000 (at m/z 400), root-mean-square mass measurement accuracy below 100 ppb, and dynamic range per pixel over 500:1 were obtained from the direct analysis of biological tissue sections. Molecular features with m/z differences as small as 1.79 mDa were resolved and identified with high mass accuracy. These features allow for the separation and identification of lipids to the underlying structures of tissues. The unique molecular detail, accuracy, sensitivity, and dynamic range combined in a 21T MALDI FT-ICR MSI experiment enable researchers to visualize molecular structures in complex tissues that have remained hidden until now. The instrument described allows for future innovative, such as high-end studies to unravel the complexity of biological, geological, and engineered organic material surfaces with an unsurpassed detail.
format Online
Article
Text
id pubmed-7031845
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-70318452020-02-21 Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS Bowman, Andrew P. Blakney, Greg T. Hendrickson, Christopher L. Ellis, Shane R. Heeren, Ron M. A. Smith, Donald F. Anal Chem [Image: see text] Detailed characterization of complex biological surfaces by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) requires instrumentation that is capable of high mass resolving power, mass accuracy, and dynamic range. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) offers the highest mass spectral performance for MALDI MSI experiments, and often reveals molecular features that are unresolved on lower performance instrumentation. Higher magnetic field strength improves all performance characteristics of FT-ICR; mass resolving power improves linearly, while mass accuracy and dynamic range improve quadratically with magnetic field strength. Here, MALDI MSI at 21T is demonstrated for the first time: mass resolving power in excess of 1 600 000 (at m/z 400), root-mean-square mass measurement accuracy below 100 ppb, and dynamic range per pixel over 500:1 were obtained from the direct analysis of biological tissue sections. Molecular features with m/z differences as small as 1.79 mDa were resolved and identified with high mass accuracy. These features allow for the separation and identification of lipids to the underlying structures of tissues. The unique molecular detail, accuracy, sensitivity, and dynamic range combined in a 21T MALDI FT-ICR MSI experiment enable researchers to visualize molecular structures in complex tissues that have remained hidden until now. The instrument described allows for future innovative, such as high-end studies to unravel the complexity of biological, geological, and engineered organic material surfaces with an unsurpassed detail. American Chemical Society 2020-01-19 2020-02-18 /pmc/articles/PMC7031845/ /pubmed/31955581 http://dx.doi.org/10.1021/acs.analchem.9b04768 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Bowman, Andrew P.
Blakney, Greg T.
Hendrickson, Christopher L.
Ellis, Shane R.
Heeren, Ron M. A.
Smith, Donald F.
Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
title Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
title_full Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
title_fullStr Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
title_full_unstemmed Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
title_short Ultra-High Mass Resolving Power, Mass Accuracy, and Dynamic Range MALDI Mass Spectrometry Imaging by 21-T FT-ICR MS
title_sort ultra-high mass resolving power, mass accuracy, and dynamic range maldi mass spectrometry imaging by 21-t ft-icr ms
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031845/
https://www.ncbi.nlm.nih.gov/pubmed/31955581
http://dx.doi.org/10.1021/acs.analchem.9b04768
work_keys_str_mv AT bowmanandrewp ultrahighmassresolvingpowermassaccuracyanddynamicrangemaldimassspectrometryimagingby21tfticrms
AT blakneygregt ultrahighmassresolvingpowermassaccuracyanddynamicrangemaldimassspectrometryimagingby21tfticrms
AT hendricksonchristopherl ultrahighmassresolvingpowermassaccuracyanddynamicrangemaldimassspectrometryimagingby21tfticrms
AT ellisshaner ultrahighmassresolvingpowermassaccuracyanddynamicrangemaldimassspectrometryimagingby21tfticrms
AT heerenronma ultrahighmassresolvingpowermassaccuracyanddynamicrangemaldimassspectrometryimagingby21tfticrms
AT smithdonaldf ultrahighmassresolvingpowermassaccuracyanddynamicrangemaldimassspectrometryimagingby21tfticrms