Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design

In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box–Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles...

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Detalles Bibliográficos
Autores principales: Li, Wenpan, Jing, Shasha, Xin, Xiu, Zhang, Xirui, Chen, Kang, Chen, Dawei, Hu, Haiyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shenyang Pharmaceutical University 2017
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7032106/
https://www.ncbi.nlm.nih.gov/pubmed/32104328
http://dx.doi.org/10.1016/j.ajps.2016.09.006
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author Li, Wenpan
Jing, Shasha
Xin, Xiu
Zhang, Xirui
Chen, Kang
Chen, Dawei
Hu, Haiyang
author_facet Li, Wenpan
Jing, Shasha
Xin, Xiu
Zhang, Xirui
Chen, Kang
Chen, Dawei
Hu, Haiyang
author_sort Li, Wenpan
collection PubMed
description In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box–Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles were found to be 60.23 ± 4.72 nm, polydispersity index was 0.252 and pDNA encapsulate efficiency was more than 90%. The optimized CaP/pDNA nanoparticles have pH sensitivity and biocompatibility. Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency.
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spelling pubmed-70321062020-02-26 Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design Li, Wenpan Jing, Shasha Xin, Xiu Zhang, Xirui Chen, Kang Chen, Dawei Hu, Haiyang Asian J Pharm Sci Original Research Article In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box–Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles were found to be 60.23 ± 4.72 nm, polydispersity index was 0.252 and pDNA encapsulate efficiency was more than 90%. The optimized CaP/pDNA nanoparticles have pH sensitivity and biocompatibility. Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency. Shenyang Pharmaceutical University 2017-03 2016-11-04 /pmc/articles/PMC7032106/ /pubmed/32104328 http://dx.doi.org/10.1016/j.ajps.2016.09.006 Text en © 2017 Shenyang Pharmaceutical University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Li, Wenpan
Jing, Shasha
Xin, Xiu
Zhang, Xirui
Chen, Kang
Chen, Dawei
Hu, Haiyang
Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design
title Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design
title_full Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design
title_fullStr Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design
title_full_unstemmed Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design
title_short Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design
title_sort preparation of cap/pdna nanoparticles by reverse micro-emulsion method: optimization of formulation variables using experimental design
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7032106/
https://www.ncbi.nlm.nih.gov/pubmed/32104328
http://dx.doi.org/10.1016/j.ajps.2016.09.006
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