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Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model
Bacterial EVs have been related to inter-kingdom communication between probiotic/pathogenic bacteria and their hosts. Our aim was to investigate the transcytosis process of B. subtilis EVs using an in vitro intestinal epithelial cell model. In this study, using Confocal Laser Scanning Microscopy, we...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033168/ https://www.ncbi.nlm.nih.gov/pubmed/32080346 http://dx.doi.org/10.1038/s41598-020-60077-4 |
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author | Rubio, Ana Paula Domínguez Martínez, Jimena Palavecino, Marcos Fuentes, Federico López, Christian Miquel Sánchez Marcilla, Antonio Pérez, Oscar Edgardo Piuri, Mariana |
author_facet | Rubio, Ana Paula Domínguez Martínez, Jimena Palavecino, Marcos Fuentes, Federico López, Christian Miquel Sánchez Marcilla, Antonio Pérez, Oscar Edgardo Piuri, Mariana |
author_sort | Rubio, Ana Paula Domínguez |
collection | PubMed |
description | Bacterial EVs have been related to inter-kingdom communication between probiotic/pathogenic bacteria and their hosts. Our aim was to investigate the transcytosis process of B. subtilis EVs using an in vitro intestinal epithelial cell model. In this study, using Confocal Laser Scanning Microscopy, we report that uptake and internalization of CFSE-labeled B. subtilis EVs (115 nm ± 27 nm) by Caco-2 cells are time-dependent. To study the transcytosis process we used a transwell system and EVs were quantified in the lower chamber by Fluorescence and Nanoparticle Tracking Analysis measurements. Intact EVs are transported across a polarized cell monolayer at 60–120 min and increased after 240 min with an estimated average uptake efficiency of 30% and this process is dose-dependent. EVs movement into intestinal epithelial cells was mainly through Z axis and scarcely on X and Y axis. This work demonstrates that EVs could be transported across the gastrointestinal epithelium. We speculate this mechanism could be the first step allowing EVs to reach the bloodstream for further delivery up to extraintestinal tissues and organs. The expression and further encapsulation of bioactive molecules into natural nanoparticles produced by probiotic bacteria could have practical implications in food, nutraceuticals and clinical therapies. |
format | Online Article Text |
id | pubmed-7033168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70331682020-02-28 Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model Rubio, Ana Paula Domínguez Martínez, Jimena Palavecino, Marcos Fuentes, Federico López, Christian Miquel Sánchez Marcilla, Antonio Pérez, Oscar Edgardo Piuri, Mariana Sci Rep Article Bacterial EVs have been related to inter-kingdom communication between probiotic/pathogenic bacteria and their hosts. Our aim was to investigate the transcytosis process of B. subtilis EVs using an in vitro intestinal epithelial cell model. In this study, using Confocal Laser Scanning Microscopy, we report that uptake and internalization of CFSE-labeled B. subtilis EVs (115 nm ± 27 nm) by Caco-2 cells are time-dependent. To study the transcytosis process we used a transwell system and EVs were quantified in the lower chamber by Fluorescence and Nanoparticle Tracking Analysis measurements. Intact EVs are transported across a polarized cell monolayer at 60–120 min and increased after 240 min with an estimated average uptake efficiency of 30% and this process is dose-dependent. EVs movement into intestinal epithelial cells was mainly through Z axis and scarcely on X and Y axis. This work demonstrates that EVs could be transported across the gastrointestinal epithelium. We speculate this mechanism could be the first step allowing EVs to reach the bloodstream for further delivery up to extraintestinal tissues and organs. The expression and further encapsulation of bioactive molecules into natural nanoparticles produced by probiotic bacteria could have practical implications in food, nutraceuticals and clinical therapies. Nature Publishing Group UK 2020-02-20 /pmc/articles/PMC7033168/ /pubmed/32080346 http://dx.doi.org/10.1038/s41598-020-60077-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Rubio, Ana Paula Domínguez Martínez, Jimena Palavecino, Marcos Fuentes, Federico López, Christian Miquel Sánchez Marcilla, Antonio Pérez, Oscar Edgardo Piuri, Mariana Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model |
title | Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model |
title_full | Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model |
title_fullStr | Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model |
title_full_unstemmed | Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model |
title_short | Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model |
title_sort | transcytosis of bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033168/ https://www.ncbi.nlm.nih.gov/pubmed/32080346 http://dx.doi.org/10.1038/s41598-020-60077-4 |
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