Cargando…

Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy

The rapid qualitative assessment of surface markers on cancer cells can allow for point-of-care prediction of patient response to various cancer drugs. Preclinical studies targeting cells with an antibody to “activated” matriptase conjugated to a potent toxin show promise as a selective treatment fo...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Zhongtian, Lin, Siang-Yo, Xie, Pengfei, Lin, Chen-Yong, Rather, Gulam M., Bertino, Joseph R., Javanmard, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033175/
https://www.ncbi.nlm.nih.gov/pubmed/32080205
http://dx.doi.org/10.1038/s41598-020-57540-7
_version_ 1783499607774330880
author Lin, Zhongtian
Lin, Siang-Yo
Xie, Pengfei
Lin, Chen-Yong
Rather, Gulam M.
Bertino, Joseph R.
Javanmard, Mehdi
author_facet Lin, Zhongtian
Lin, Siang-Yo
Xie, Pengfei
Lin, Chen-Yong
Rather, Gulam M.
Bertino, Joseph R.
Javanmard, Mehdi
author_sort Lin, Zhongtian
collection PubMed
description The rapid qualitative assessment of surface markers on cancer cells can allow for point-of-care prediction of patient response to various cancer drugs. Preclinical studies targeting cells with an antibody to “activated” matriptase conjugated to a potent toxin show promise as a selective treatment for a variety of solid tumors. In this paper, we implemented a novel technique for electrical detection of proteins on surfaces of cancer cells using multi-frequency microfluidic impedance cytometry. The biosensor, consists of two gold microelectrodes on a glass substrate embedded in a PDMS microfluidic channel, is used in conjugation with immuno-magnetic separation of cancer cells, and is capable of differentiating between bare magnetic beads, cancer cells and bead-cell aggregates based on their various impedance and frequency responses. We demonstrated proof-of-concept based on detection of “activated” matriptase proteins on the surface of cultured Mantle cells.
format Online
Article
Text
id pubmed-7033175
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-70331752020-02-28 Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy Lin, Zhongtian Lin, Siang-Yo Xie, Pengfei Lin, Chen-Yong Rather, Gulam M. Bertino, Joseph R. Javanmard, Mehdi Sci Rep Article The rapid qualitative assessment of surface markers on cancer cells can allow for point-of-care prediction of patient response to various cancer drugs. Preclinical studies targeting cells with an antibody to “activated” matriptase conjugated to a potent toxin show promise as a selective treatment for a variety of solid tumors. In this paper, we implemented a novel technique for electrical detection of proteins on surfaces of cancer cells using multi-frequency microfluidic impedance cytometry. The biosensor, consists of two gold microelectrodes on a glass substrate embedded in a PDMS microfluidic channel, is used in conjugation with immuno-magnetic separation of cancer cells, and is capable of differentiating between bare magnetic beads, cancer cells and bead-cell aggregates based on their various impedance and frequency responses. We demonstrated proof-of-concept based on detection of “activated” matriptase proteins on the surface of cultured Mantle cells. Nature Publishing Group UK 2020-02-20 /pmc/articles/PMC7033175/ /pubmed/32080205 http://dx.doi.org/10.1038/s41598-020-57540-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lin, Zhongtian
Lin, Siang-Yo
Xie, Pengfei
Lin, Chen-Yong
Rather, Gulam M.
Bertino, Joseph R.
Javanmard, Mehdi
Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
title Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
title_full Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
title_fullStr Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
title_full_unstemmed Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
title_short Rapid Assessment of Surface Markers on Cancer Cells Using Immuno-Magnetic Separation and Multi-frequency Impedance Cytometry for Targeted Therapy
title_sort rapid assessment of surface markers on cancer cells using immuno-magnetic separation and multi-frequency impedance cytometry for targeted therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033175/
https://www.ncbi.nlm.nih.gov/pubmed/32080205
http://dx.doi.org/10.1038/s41598-020-57540-7
work_keys_str_mv AT linzhongtian rapidassessmentofsurfacemarkersoncancercellsusingimmunomagneticseparationandmultifrequencyimpedancecytometryfortargetedtherapy
AT linsiangyo rapidassessmentofsurfacemarkersoncancercellsusingimmunomagneticseparationandmultifrequencyimpedancecytometryfortargetedtherapy
AT xiepengfei rapidassessmentofsurfacemarkersoncancercellsusingimmunomagneticseparationandmultifrequencyimpedancecytometryfortargetedtherapy
AT linchenyong rapidassessmentofsurfacemarkersoncancercellsusingimmunomagneticseparationandmultifrequencyimpedancecytometryfortargetedtherapy
AT rathergulamm rapidassessmentofsurfacemarkersoncancercellsusingimmunomagneticseparationandmultifrequencyimpedancecytometryfortargetedtherapy
AT bertinojosephr rapidassessmentofsurfacemarkersoncancercellsusingimmunomagneticseparationandmultifrequencyimpedancecytometryfortargetedtherapy
AT javanmardmehdi rapidassessmentofsurfacemarkersoncancercellsusingimmunomagneticseparationandmultifrequencyimpedancecytometryfortargetedtherapy