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Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis
Triptolide is a trace natural product of Tripterygium wilfordii. It has antitumor activities, particularly against pancreatic cancer cells. Identification of genes and elucidation of the biosynthetic pathway leading to triptolide are the prerequisite for heterologous bioproduction. Here, we report a...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033203/ https://www.ncbi.nlm.nih.gov/pubmed/32080175 http://dx.doi.org/10.1038/s41467-020-14776-1 |
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author | Tu, Lichan Su, Ping Zhang, Zhongren Gao, Linhui Wang, Jiadian Hu, Tianyuan Zhou, Jiawei Zhang, Yifeng Zhao, Yujun Liu, Yuan Song, Yadi Tong, Yuru Lu, Yun Yang, Jian Xu, Cao Jia, Meirong Peters, Reuben J. Huang, Luqi Gao, Wei |
author_facet | Tu, Lichan Su, Ping Zhang, Zhongren Gao, Linhui Wang, Jiadian Hu, Tianyuan Zhou, Jiawei Zhang, Yifeng Zhao, Yujun Liu, Yuan Song, Yadi Tong, Yuru Lu, Yun Yang, Jian Xu, Cao Jia, Meirong Peters, Reuben J. Huang, Luqi Gao, Wei |
author_sort | Tu, Lichan |
collection | PubMed |
description | Triptolide is a trace natural product of Tripterygium wilfordii. It has antitumor activities, particularly against pancreatic cancer cells. Identification of genes and elucidation of the biosynthetic pathway leading to triptolide are the prerequisite for heterologous bioproduction. Here, we report a reference-grade genome of T. wilfordii with a contig N50 of 4.36 Mb. We show that copy numbers of triptolide biosynthetic pathway genes are impacted by a recent whole-genome triplication event. We further integrate genomic, transcriptomic, and metabolomic data to map a gene-to-metabolite network. This leads to the identification of a cytochrome P450 (CYP728B70) that can catalyze oxidation of a methyl to the acid moiety of dehydroabietic acid in triptolide biosynthesis. We think the genomic resource and the candidate genes reported here set the foundation to fully reveal triptolide biosynthetic pathway and consequently the heterologous bioproduction. |
format | Online Article Text |
id | pubmed-7033203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70332032020-03-04 Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis Tu, Lichan Su, Ping Zhang, Zhongren Gao, Linhui Wang, Jiadian Hu, Tianyuan Zhou, Jiawei Zhang, Yifeng Zhao, Yujun Liu, Yuan Song, Yadi Tong, Yuru Lu, Yun Yang, Jian Xu, Cao Jia, Meirong Peters, Reuben J. Huang, Luqi Gao, Wei Nat Commun Article Triptolide is a trace natural product of Tripterygium wilfordii. It has antitumor activities, particularly against pancreatic cancer cells. Identification of genes and elucidation of the biosynthetic pathway leading to triptolide are the prerequisite for heterologous bioproduction. Here, we report a reference-grade genome of T. wilfordii with a contig N50 of 4.36 Mb. We show that copy numbers of triptolide biosynthetic pathway genes are impacted by a recent whole-genome triplication event. We further integrate genomic, transcriptomic, and metabolomic data to map a gene-to-metabolite network. This leads to the identification of a cytochrome P450 (CYP728B70) that can catalyze oxidation of a methyl to the acid moiety of dehydroabietic acid in triptolide biosynthesis. We think the genomic resource and the candidate genes reported here set the foundation to fully reveal triptolide biosynthetic pathway and consequently the heterologous bioproduction. Nature Publishing Group UK 2020-02-20 /pmc/articles/PMC7033203/ /pubmed/32080175 http://dx.doi.org/10.1038/s41467-020-14776-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tu, Lichan Su, Ping Zhang, Zhongren Gao, Linhui Wang, Jiadian Hu, Tianyuan Zhou, Jiawei Zhang, Yifeng Zhao, Yujun Liu, Yuan Song, Yadi Tong, Yuru Lu, Yun Yang, Jian Xu, Cao Jia, Meirong Peters, Reuben J. Huang, Luqi Gao, Wei Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis |
title | Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis |
title_full | Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis |
title_fullStr | Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis |
title_full_unstemmed | Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis |
title_short | Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis |
title_sort | genome of tripterygium wilfordii and identification of cytochrome p450 involved in triptolide biosynthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033203/ https://www.ncbi.nlm.nih.gov/pubmed/32080175 http://dx.doi.org/10.1038/s41467-020-14776-1 |
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