Cargando…

Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models

BACKGROUND: The aim of this study was to clarify the objective therapeutic effects of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models. METHODS: Two osteochondral defects (diameters: 3.0 and 5.0 mm) were created on each patellar groove in...

Descripción completa

Detalles Bibliográficos
Autores principales: Onodera, Tomohiro, Baba, Rikiya, Kasahara, Yasuhiko, Tsuda, Toshihiko, Iwasaki, Norimasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033291/
https://www.ncbi.nlm.nih.gov/pubmed/32110684
http://dx.doi.org/10.1016/j.reth.2020.01.001
_version_ 1783499634394529792
author Onodera, Tomohiro
Baba, Rikiya
Kasahara, Yasuhiko
Tsuda, Toshihiko
Iwasaki, Norimasa
author_facet Onodera, Tomohiro
Baba, Rikiya
Kasahara, Yasuhiko
Tsuda, Toshihiko
Iwasaki, Norimasa
author_sort Onodera, Tomohiro
collection PubMed
description BACKGROUND: The aim of this study was to clarify the objective therapeutic effects of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models. METHODS: Two osteochondral defects (diameters: 3.0 and 5.0 mm) were created on each patellar groove in both knees of 10 dogs. Defects were divided into four groups (n = 10 each): Group 1, untreated 3.0-mm defect; Group 2, 3.0-mm defect with UPAL gel; Group 3, untreated 5.0-mm defect; and Group 4, 5.0-mm defect with UPAL gel. All surgical procedures were performed by individuals unfamiliar with the technique at an independent institution. Articular surfaces were evaluated grossly and histologically at 27 weeks after operation. RESULTS: UPAL gel-treated osteochondral defects showed significantly improved gross appearance in Group 4 and histological appearance in Groups 2 and 4. Reparative tissues in the 3.0-mm defect with UPAL gel were replaced by hyaline-like cartilage tissue. The 5.0-mm defects with UPAL gel were mostly covered with fibrocartilaginous tissue, whereas UPAL gel-untreated defects mostly remained uncovered by any tissue. CONCLUSIONS: Although an acellular technique using UPAL gel implantation significantly enhanced osteochondral repair in canines, reparative tissues of the large defect with alginate gel comprised of fibrocartilaginous tissue. This surgical technique is effective, especially for small cartilage injuries. Further improvements are required before clinical application in cases of severe osteochondral defects in humans.
format Online
Article
Text
id pubmed-7033291
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Japanese Society for Regenerative Medicine
record_format MEDLINE/PubMed
spelling pubmed-70332912020-02-27 Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models Onodera, Tomohiro Baba, Rikiya Kasahara, Yasuhiko Tsuda, Toshihiko Iwasaki, Norimasa Regen Ther Original Article BACKGROUND: The aim of this study was to clarify the objective therapeutic effects of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models. METHODS: Two osteochondral defects (diameters: 3.0 and 5.0 mm) were created on each patellar groove in both knees of 10 dogs. Defects were divided into four groups (n = 10 each): Group 1, untreated 3.0-mm defect; Group 2, 3.0-mm defect with UPAL gel; Group 3, untreated 5.0-mm defect; and Group 4, 5.0-mm defect with UPAL gel. All surgical procedures were performed by individuals unfamiliar with the technique at an independent institution. Articular surfaces were evaluated grossly and histologically at 27 weeks after operation. RESULTS: UPAL gel-treated osteochondral defects showed significantly improved gross appearance in Group 4 and histological appearance in Groups 2 and 4. Reparative tissues in the 3.0-mm defect with UPAL gel were replaced by hyaline-like cartilage tissue. The 5.0-mm defects with UPAL gel were mostly covered with fibrocartilaginous tissue, whereas UPAL gel-untreated defects mostly remained uncovered by any tissue. CONCLUSIONS: Although an acellular technique using UPAL gel implantation significantly enhanced osteochondral repair in canines, reparative tissues of the large defect with alginate gel comprised of fibrocartilaginous tissue. This surgical technique is effective, especially for small cartilage injuries. Further improvements are required before clinical application in cases of severe osteochondral defects in humans. Japanese Society for Regenerative Medicine 2020-02-20 /pmc/articles/PMC7033291/ /pubmed/32110684 http://dx.doi.org/10.1016/j.reth.2020.01.001 Text en © 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Onodera, Tomohiro
Baba, Rikiya
Kasahara, Yasuhiko
Tsuda, Toshihiko
Iwasaki, Norimasa
Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models
title Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models
title_full Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models
title_fullStr Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models
title_full_unstemmed Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models
title_short Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models
title_sort therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (upal) gel implantation in canine osteochondral defect models
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033291/
https://www.ncbi.nlm.nih.gov/pubmed/32110684
http://dx.doi.org/10.1016/j.reth.2020.01.001
work_keys_str_mv AT onoderatomohiro therapeuticeffectsandadaptivelimitsofanacellulartechniquebyultrapurifiedalginateupalgelimplantationincanineosteochondraldefectmodels
AT babarikiya therapeuticeffectsandadaptivelimitsofanacellulartechniquebyultrapurifiedalginateupalgelimplantationincanineosteochondraldefectmodels
AT kasaharayasuhiko therapeuticeffectsandadaptivelimitsofanacellulartechniquebyultrapurifiedalginateupalgelimplantationincanineosteochondraldefectmodels
AT tsudatoshihiko therapeuticeffectsandadaptivelimitsofanacellulartechniquebyultrapurifiedalginateupalgelimplantationincanineosteochondraldefectmodels
AT iwasakinorimasa therapeuticeffectsandadaptivelimitsofanacellulartechniquebyultrapurifiedalginateupalgelimplantationincanineosteochondraldefectmodels