Cargando…

Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin

BACKGROUND: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reacti...

Descripción completa

Detalles Bibliográficos
Autores principales: Jang, Ik-Soon, Jo, Eunbi, Park, Soo Jung, Baek, Su Jeong, Hwang, In-Hu, Kang, Hyun Mi, Lee, Je-Ho, Kwon, Joseph, Son, Junik, Kwon, Ho Jeong, Choi, Jong-Soon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033328/
https://www.ncbi.nlm.nih.gov/pubmed/32148389
http://dx.doi.org/10.1016/j.jgr.2018.07.008
_version_ 1783499642918404096
author Jang, Ik-Soon
Jo, Eunbi
Park, Soo Jung
Baek, Su Jeong
Hwang, In-Hu
Kang, Hyun Mi
Lee, Je-Ho
Kwon, Joseph
Son, Junik
Kwon, Ho Jeong
Choi, Jong-Soon
author_facet Jang, Ik-Soon
Jo, Eunbi
Park, Soo Jung
Baek, Su Jeong
Hwang, In-Hu
Kang, Hyun Mi
Lee, Je-Ho
Kwon, Joseph
Son, Junik
Kwon, Ho Jeong
Choi, Jong-Soon
author_sort Jang, Ik-Soon
collection PubMed
description BACKGROUND: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. METHODS: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. RESULTS: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. CONCLUSION: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3.
format Online
Article
Text
id pubmed-7033328
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-70333282020-03-06 Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin Jang, Ik-Soon Jo, Eunbi Park, Soo Jung Baek, Su Jeong Hwang, In-Hu Kang, Hyun Mi Lee, Je-Ho Kwon, Joseph Son, Junik Kwon, Ho Jeong Choi, Jong-Soon J Ginseng Res Pharmacology and Physiology BACKGROUND: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. METHODS: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. RESULTS: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. CONCLUSION: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3. Elsevier 2020-01 2018-08-13 /pmc/articles/PMC7033328/ /pubmed/32148389 http://dx.doi.org/10.1016/j.jgr.2018.07.008 Text en © 2018 The Korean Society of Ginseng. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Pharmacology and Physiology
Jang, Ik-Soon
Jo, Eunbi
Park, Soo Jung
Baek, Su Jeong
Hwang, In-Hu
Kang, Hyun Mi
Lee, Je-Ho
Kwon, Joseph
Son, Junik
Kwon, Ho Jeong
Choi, Jong-Soon
Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin
title Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin
title_full Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin
title_fullStr Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin
title_full_unstemmed Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin
title_short Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin
title_sort proteomic analyses reveal that ginsenoside rg3(s) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin
topic Pharmacology and Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033328/
https://www.ncbi.nlm.nih.gov/pubmed/32148389
http://dx.doi.org/10.1016/j.jgr.2018.07.008
work_keys_str_mv AT jangiksoon proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT joeunbi proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT parksoojung proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT baeksujeong proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT hwanginhu proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT kanghyunmi proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT leejeho proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT kwonjoseph proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT sonjunik proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT kwonhojeong proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin
AT choijongsoon proteomicanalysesrevealthatginsenosiderg3spartiallyreversescellularsenescenceinhumandermalfibroblastsbyinducingperoxiredoxin