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Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system

BACKGROUND: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new...

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Autores principales: Wei, Yingqin, Hou, Baojuan, Fang, Haiyan, Sun, Xinjie, Ma, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033334/
https://www.ncbi.nlm.nih.gov/pubmed/32148388
http://dx.doi.org/10.1016/j.jgr.2018.08.001
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author Wei, Yingqin
Hou, Baojuan
Fang, Haiyan
Sun, Xinjie
Ma, Feng
author_facet Wei, Yingqin
Hou, Baojuan
Fang, Haiyan
Sun, Xinjie
Ma, Feng
author_sort Wei, Yingqin
collection PubMed
description BACKGROUND: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOE method for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium was established in this article. METHODS: The SOE system comprising ethanol and sodium carbonate was selected to extract ginsenosides from the enzymatic hydrolysates of Panax quinquefolium, and HPLC was applied to analyze the ginsenosides. RESULTS: The optimized extraction conditions were as follows: the aqueous two-phase extraction system comprising ethanol, sodium carbonate, ethanol concentration of 41.51%, and the mass percent of sodium carbonate of 7.9% in the extraction system under the experimental condition. Extraction time had minor influence on extraction efficiency of ginsenosides. The results also showed that the extraction efficiencies of three ginsenosides were all more than 90.0% only in a single step. CONCLUSION: The proposed method had been successfully applied to determine ginsenosides in enzymatic hydrolysate and demonstrated as a powerful technique for separating and purifying ginsenosides in complex samples.
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spelling pubmed-70333342020-03-06 Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system Wei, Yingqin Hou, Baojuan Fang, Haiyan Sun, Xinjie Ma, Feng J Ginseng Res Chemistry BACKGROUND: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOE method for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium was established in this article. METHODS: The SOE system comprising ethanol and sodium carbonate was selected to extract ginsenosides from the enzymatic hydrolysates of Panax quinquefolium, and HPLC was applied to analyze the ginsenosides. RESULTS: The optimized extraction conditions were as follows: the aqueous two-phase extraction system comprising ethanol, sodium carbonate, ethanol concentration of 41.51%, and the mass percent of sodium carbonate of 7.9% in the extraction system under the experimental condition. Extraction time had minor influence on extraction efficiency of ginsenosides. The results also showed that the extraction efficiencies of three ginsenosides were all more than 90.0% only in a single step. CONCLUSION: The proposed method had been successfully applied to determine ginsenosides in enzymatic hydrolysate and demonstrated as a powerful technique for separating and purifying ginsenosides in complex samples. Elsevier 2020-01 2018-08-08 /pmc/articles/PMC7033334/ /pubmed/32148388 http://dx.doi.org/10.1016/j.jgr.2018.08.001 Text en © 2018 The Korean Society of Ginseng. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Chemistry
Wei, Yingqin
Hou, Baojuan
Fang, Haiyan
Sun, Xinjie
Ma, Feng
Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system
title Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system
title_full Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system
title_fullStr Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system
title_full_unstemmed Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system
title_short Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system
title_sort salting-out extraction of ginsenosides from the enzymatic hydrolysates of panax quinquefolium based on ethanol/sodium carbonate system
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033334/
https://www.ncbi.nlm.nih.gov/pubmed/32148388
http://dx.doi.org/10.1016/j.jgr.2018.08.001
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