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Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos

E804, a derivative of indirubin, have multi-biological activities such as anticancer and anti-inflammatory activities, but little is known about its developmental toxicity. In this study, we investigated the toxicity of E804 on the developments of zebrafish embryos. Our results showed that E804 trea...

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Autores principales: Wang, Rongchun, Liu, Kechun, Zhang, Yun, Chen, Xiqiang, Wang, Xue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033426/
https://www.ncbi.nlm.nih.gov/pubmed/32116709
http://dx.doi.org/10.3389/fphar.2020.00032
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author Wang, Rongchun
Liu, Kechun
Zhang, Yun
Chen, Xiqiang
Wang, Xue
author_facet Wang, Rongchun
Liu, Kechun
Zhang, Yun
Chen, Xiqiang
Wang, Xue
author_sort Wang, Rongchun
collection PubMed
description E804, a derivative of indirubin, have multi-biological activities such as anticancer and anti-inflammatory activities, but little is known about its developmental toxicity. In this study, we investigated the toxicity of E804 on the developments of zebrafish embryos. Our results showed that E804 treatment caused a significant increase of the malformation rate compared with the control groups. Pericardial edema and curved body shape were the most morphological abnormalities observed in E804-treated group. The hatching rates and body length of the zebrafish larvae was significantly decreased in E804-treated groups. E804 also affect the development of heart, liver, phagocytes and vascular formation. Further studies showed that the level of reactive oxygen species was significantly increased. The activity of total superoxide dismutase decreased and the concentration of malondialdehyde were increased. Much more apoptotic cells were detected in E804-treated group, compared with the control. In addition, gene-expression results showed that the pathways of oxidative stress and apoptosis were provoked in E804 treated groups. Taken together, our findings will be helpful to understanding E804-induced developmental toxicity and the underlying mechanism.
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spelling pubmed-70334262020-02-28 Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos Wang, Rongchun Liu, Kechun Zhang, Yun Chen, Xiqiang Wang, Xue Front Pharmacol Pharmacology E804, a derivative of indirubin, have multi-biological activities such as anticancer and anti-inflammatory activities, but little is known about its developmental toxicity. In this study, we investigated the toxicity of E804 on the developments of zebrafish embryos. Our results showed that E804 treatment caused a significant increase of the malformation rate compared with the control groups. Pericardial edema and curved body shape were the most morphological abnormalities observed in E804-treated group. The hatching rates and body length of the zebrafish larvae was significantly decreased in E804-treated groups. E804 also affect the development of heart, liver, phagocytes and vascular formation. Further studies showed that the level of reactive oxygen species was significantly increased. The activity of total superoxide dismutase decreased and the concentration of malondialdehyde were increased. Much more apoptotic cells were detected in E804-treated group, compared with the control. In addition, gene-expression results showed that the pathways of oxidative stress and apoptosis were provoked in E804 treated groups. Taken together, our findings will be helpful to understanding E804-induced developmental toxicity and the underlying mechanism. Frontiers Media S.A. 2020-02-14 /pmc/articles/PMC7033426/ /pubmed/32116709 http://dx.doi.org/10.3389/fphar.2020.00032 Text en Copyright © 2020 Wang, Liu, Zhang, Chen and Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wang, Rongchun
Liu, Kechun
Zhang, Yun
Chen, Xiqiang
Wang, Xue
Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos
title Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos
title_full Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos
title_fullStr Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos
title_full_unstemmed Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos
title_short Evaluation of the Developmental Toxicity Induced by E804 in Zebrafish Embryos
title_sort evaluation of the developmental toxicity induced by e804 in zebrafish embryos
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033426/
https://www.ncbi.nlm.nih.gov/pubmed/32116709
http://dx.doi.org/10.3389/fphar.2020.00032
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