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Advanced Strategies for Production of Natural Products in Yeast

Natural products account for more than 50% of all small-molecule pharmaceutical agents currently in clinical use. However, low availability often becomes problematic when a bioactive natural product is promising to become a pharmaceutical or leading compound. Advances in synthetic biology and metabo...

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Detalles Bibliográficos
Autores principales: Chen, Ruibing, Yang, Shan, Zhang, Lei, Zhou, Yongjin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033514/
https://www.ncbi.nlm.nih.gov/pubmed/32087574
http://dx.doi.org/10.1016/j.isci.2020.100879
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author Chen, Ruibing
Yang, Shan
Zhang, Lei
Zhou, Yongjin J.
author_facet Chen, Ruibing
Yang, Shan
Zhang, Lei
Zhou, Yongjin J.
author_sort Chen, Ruibing
collection PubMed
description Natural products account for more than 50% of all small-molecule pharmaceutical agents currently in clinical use. However, low availability often becomes problematic when a bioactive natural product is promising to become a pharmaceutical or leading compound. Advances in synthetic biology and metabolic engineering provide a feasible solution for sustainable supply of these compounds. In this review, we have summarized current progress in engineering yeast cell factories for production of natural products, including terpenoids, alkaloids, and phenylpropanoids. We then discuss advanced strategies in metabolic engineering at three different dimensions, including point, line, and plane (corresponding to the individual enzymes and cofactors, metabolic pathways, and the global cellular network). In particular, we comprehensively discuss how to engineer cofactor biosynthesis for enhancing the biosynthesis efficiency, other than the enzyme activity. Finally, current challenges and perspective are also discussed for future engineering direction.
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spelling pubmed-70335142020-02-24 Advanced Strategies for Production of Natural Products in Yeast Chen, Ruibing Yang, Shan Zhang, Lei Zhou, Yongjin J. iScience Review Natural products account for more than 50% of all small-molecule pharmaceutical agents currently in clinical use. However, low availability often becomes problematic when a bioactive natural product is promising to become a pharmaceutical or leading compound. Advances in synthetic biology and metabolic engineering provide a feasible solution for sustainable supply of these compounds. In this review, we have summarized current progress in engineering yeast cell factories for production of natural products, including terpenoids, alkaloids, and phenylpropanoids. We then discuss advanced strategies in metabolic engineering at three different dimensions, including point, line, and plane (corresponding to the individual enzymes and cofactors, metabolic pathways, and the global cellular network). In particular, we comprehensively discuss how to engineer cofactor biosynthesis for enhancing the biosynthesis efficiency, other than the enzyme activity. Finally, current challenges and perspective are also discussed for future engineering direction. Elsevier 2020-02-01 /pmc/articles/PMC7033514/ /pubmed/32087574 http://dx.doi.org/10.1016/j.isci.2020.100879 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review
Chen, Ruibing
Yang, Shan
Zhang, Lei
Zhou, Yongjin J.
Advanced Strategies for Production of Natural Products in Yeast
title Advanced Strategies for Production of Natural Products in Yeast
title_full Advanced Strategies for Production of Natural Products in Yeast
title_fullStr Advanced Strategies for Production of Natural Products in Yeast
title_full_unstemmed Advanced Strategies for Production of Natural Products in Yeast
title_short Advanced Strategies for Production of Natural Products in Yeast
title_sort advanced strategies for production of natural products in yeast
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033514/
https://www.ncbi.nlm.nih.gov/pubmed/32087574
http://dx.doi.org/10.1016/j.isci.2020.100879
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