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Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018

Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 201...

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Autores principales: Wang, Yanqun, Li, Xin, Liu, Wenkuan, Gan, Mian, Zhang, Lu, Wang, Jin, Zhang, Zhaoyong, Zhu, Airu, Li, Fang, Sun, Jing, Zhang, Guoxian, Zhuang, Zhen, Luo, Jiaying, Chen, Dehui, Qiu, Shuyan, Zhang, Li, Xu, Duo, Mok, Chris Ka Pun, Zhang, Fuchun, Zhao, Jingxian, Zhou, Rong, Zhao, Jincun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034077/
https://www.ncbi.nlm.nih.gov/pubmed/31996093
http://dx.doi.org/10.1080/22221751.2020.1717999
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author Wang, Yanqun
Li, Xin
Liu, Wenkuan
Gan, Mian
Zhang, Lu
Wang, Jin
Zhang, Zhaoyong
Zhu, Airu
Li, Fang
Sun, Jing
Zhang, Guoxian
Zhuang, Zhen
Luo, Jiaying
Chen, Dehui
Qiu, Shuyan
Zhang, Li
Xu, Duo
Mok, Chris Ka Pun
Zhang, Fuchun
Zhao, Jingxian
Zhou, Rong
Zhao, Jincun
author_facet Wang, Yanqun
Li, Xin
Liu, Wenkuan
Gan, Mian
Zhang, Lu
Wang, Jin
Zhang, Zhaoyong
Zhu, Airu
Li, Fang
Sun, Jing
Zhang, Guoxian
Zhuang, Zhen
Luo, Jiaying
Chen, Dehui
Qiu, Shuyan
Zhang, Li
Xu, Duo
Mok, Chris Ka Pun
Zhang, Fuchun
Zhao, Jingxian
Zhou, Rong
Zhao, Jincun
author_sort Wang, Yanqun
collection PubMed
description Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans.
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spelling pubmed-70340772020-03-03 Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018 Wang, Yanqun Li, Xin Liu, Wenkuan Gan, Mian Zhang, Lu Wang, Jin Zhang, Zhaoyong Zhu, Airu Li, Fang Sun, Jing Zhang, Guoxian Zhuang, Zhen Luo, Jiaying Chen, Dehui Qiu, Shuyan Zhang, Li Xu, Duo Mok, Chris Ka Pun Zhang, Fuchun Zhao, Jingxian Zhou, Rong Zhao, Jincun Emerg Microbes Infect Article Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans. Taylor & Francis 2020-01-29 /pmc/articles/PMC7034077/ /pubmed/31996093 http://dx.doi.org/10.1080/22221751.2020.1717999 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Wang, Yanqun
Li, Xin
Liu, Wenkuan
Gan, Mian
Zhang, Lu
Wang, Jin
Zhang, Zhaoyong
Zhu, Airu
Li, Fang
Sun, Jing
Zhang, Guoxian
Zhuang, Zhen
Luo, Jiaying
Chen, Dehui
Qiu, Shuyan
Zhang, Li
Xu, Duo
Mok, Chris Ka Pun
Zhang, Fuchun
Zhao, Jingxian
Zhou, Rong
Zhao, Jincun
Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
title Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
title_full Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
title_fullStr Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
title_full_unstemmed Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
title_short Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
title_sort discovery of a subgenotype of human coronavirus nl63 associated with severe lower respiratory tract infection in china, 2018
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034077/
https://www.ncbi.nlm.nih.gov/pubmed/31996093
http://dx.doi.org/10.1080/22221751.2020.1717999
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