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Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation
Retinoic acid can cause many types of cells, including mouse neuroblastoma Neuro-2A cells, to differentiate into neurons. However, it is still unknown whether microRNAs (miRNAs) play a role in this neuronal differentiation. To address this issue, real-time polymerase chain reaction assays were used...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034285/ https://www.ncbi.nlm.nih.gov/pubmed/31823894 http://dx.doi.org/10.4103/1673-5374.270417 |
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author | You, Qun Gong, Qiang Han, Yu-Qiao Pi, Rou Du, Yi-Jie Dong, Su-Zhen |
author_facet | You, Qun Gong, Qiang Han, Yu-Qiao Pi, Rou Du, Yi-Jie Dong, Su-Zhen |
author_sort | You, Qun |
collection | PubMed |
description | Retinoic acid can cause many types of cells, including mouse neuroblastoma Neuro-2A cells, to differentiate into neurons. However, it is still unknown whether microRNAs (miRNAs) play a role in this neuronal differentiation. To address this issue, real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2A cells. The results revealed that miR-124 and miR-9 were upregulated, while miR-125b was downregulated in retinoic acid-treated Neuro-2A cells. To identify the miRNA that may play a key role, miR-124 expression was regulated by transfection of miRNA mimics or inhibitors. Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth. Moreover, miR-124 overexpression alone caused Neuro-2A cells to differentiate into neurons, and its inhibitor could block this effect. These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2A cells. |
format | Online Article Text |
id | pubmed-7034285 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-70342852020-03-09 Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation You, Qun Gong, Qiang Han, Yu-Qiao Pi, Rou Du, Yi-Jie Dong, Su-Zhen Neural Regen Res Research Article Retinoic acid can cause many types of cells, including mouse neuroblastoma Neuro-2A cells, to differentiate into neurons. However, it is still unknown whether microRNAs (miRNAs) play a role in this neuronal differentiation. To address this issue, real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2A cells. The results revealed that miR-124 and miR-9 were upregulated, while miR-125b was downregulated in retinoic acid-treated Neuro-2A cells. To identify the miRNA that may play a key role, miR-124 expression was regulated by transfection of miRNA mimics or inhibitors. Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth. Moreover, miR-124 overexpression alone caused Neuro-2A cells to differentiate into neurons, and its inhibitor could block this effect. These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2A cells. Wolters Kluwer - Medknow 2019-12-10 /pmc/articles/PMC7034285/ /pubmed/31823894 http://dx.doi.org/10.4103/1673-5374.270417 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Research Article You, Qun Gong, Qiang Han, Yu-Qiao Pi, Rou Du, Yi-Jie Dong, Su-Zhen Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation |
title | Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation |
title_full | Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation |
title_fullStr | Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation |
title_full_unstemmed | Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation |
title_short | Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation |
title_sort | role of mir-124 in the regulation of retinoic acid-induced neuro-2a cell differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034285/ https://www.ncbi.nlm.nih.gov/pubmed/31823894 http://dx.doi.org/10.4103/1673-5374.270417 |
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