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Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation

Retinoic acid can cause many types of cells, including mouse neuroblastoma Neuro-2A cells, to differentiate into neurons. However, it is still unknown whether microRNAs (miRNAs) play a role in this neuronal differentiation. To address this issue, real-time polymerase chain reaction assays were used...

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Autores principales: You, Qun, Gong, Qiang, Han, Yu-Qiao, Pi, Rou, Du, Yi-Jie, Dong, Su-Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034285/
https://www.ncbi.nlm.nih.gov/pubmed/31823894
http://dx.doi.org/10.4103/1673-5374.270417
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author You, Qun
Gong, Qiang
Han, Yu-Qiao
Pi, Rou
Du, Yi-Jie
Dong, Su-Zhen
author_facet You, Qun
Gong, Qiang
Han, Yu-Qiao
Pi, Rou
Du, Yi-Jie
Dong, Su-Zhen
author_sort You, Qun
collection PubMed
description Retinoic acid can cause many types of cells, including mouse neuroblastoma Neuro-2A cells, to differentiate into neurons. However, it is still unknown whether microRNAs (miRNAs) play a role in this neuronal differentiation. To address this issue, real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2A cells. The results revealed that miR-124 and miR-9 were upregulated, while miR-125b was downregulated in retinoic acid-treated Neuro-2A cells. To identify the miRNA that may play a key role, miR-124 expression was regulated by transfection of miRNA mimics or inhibitors. Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth. Moreover, miR-124 overexpression alone caused Neuro-2A cells to differentiate into neurons, and its inhibitor could block this effect. These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2A cells.
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spelling pubmed-70342852020-03-09 Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation You, Qun Gong, Qiang Han, Yu-Qiao Pi, Rou Du, Yi-Jie Dong, Su-Zhen Neural Regen Res Research Article Retinoic acid can cause many types of cells, including mouse neuroblastoma Neuro-2A cells, to differentiate into neurons. However, it is still unknown whether microRNAs (miRNAs) play a role in this neuronal differentiation. To address this issue, real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2A cells. The results revealed that miR-124 and miR-9 were upregulated, while miR-125b was downregulated in retinoic acid-treated Neuro-2A cells. To identify the miRNA that may play a key role, miR-124 expression was regulated by transfection of miRNA mimics or inhibitors. Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth. Moreover, miR-124 overexpression alone caused Neuro-2A cells to differentiate into neurons, and its inhibitor could block this effect. These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2A cells. Wolters Kluwer - Medknow 2019-12-10 /pmc/articles/PMC7034285/ /pubmed/31823894 http://dx.doi.org/10.4103/1673-5374.270417 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
You, Qun
Gong, Qiang
Han, Yu-Qiao
Pi, Rou
Du, Yi-Jie
Dong, Su-Zhen
Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation
title Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation
title_full Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation
title_fullStr Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation
title_full_unstemmed Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation
title_short Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation
title_sort role of mir-124 in the regulation of retinoic acid-induced neuro-2a cell differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034285/
https://www.ncbi.nlm.nih.gov/pubmed/31823894
http://dx.doi.org/10.4103/1673-5374.270417
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