The stochastic nature of errors in next-generation sequencing of circulating cell-free DNA

Challenges with distinguishing circulating tumor DNA (ctDNA) from next-generation sequencing (NGS) artifacts limits variant searches to established solid tumor mutations. Here we show early and random PCR errors are a principal source of NGS noise that persist despite duplex molecular barcoding, rem...

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Detalles Bibliográficos
Autores principales: Nix, David A., Hellwig, Sabine, Conley, Christopher, Thomas, Alun, Fuertes, Carrie L., Hamil, Cindy L., Bhetariya, Preetida J., Garrido-Laguna, Ignacio, Marth, Gabor T., Bronner, Mary P., Underhill, Hunter R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034809/
https://www.ncbi.nlm.nih.gov/pubmed/32084206
http://dx.doi.org/10.1371/journal.pone.0229063

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