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Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin

Microorganisms produce numerous secondary metabolites (SMs) with various biological activities. Many of their encoding gene clusters are silent under standard laboratory conditions because for their activation they need the ecological context, such as the presence of other microorganisms. The true e...

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Autores principales: Stroe, Maria Cristina, Netzker, Tina, Scherlach, Kirstin, Krüger, Thomas, Hertweck, Christian, Valiante, Vito, Brakhage, Axel A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034978/
https://www.ncbi.nlm.nih.gov/pubmed/32083553
http://dx.doi.org/10.7554/eLife.52541
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author Stroe, Maria Cristina
Netzker, Tina
Scherlach, Kirstin
Krüger, Thomas
Hertweck, Christian
Valiante, Vito
Brakhage, Axel A
author_facet Stroe, Maria Cristina
Netzker, Tina
Scherlach, Kirstin
Krüger, Thomas
Hertweck, Christian
Valiante, Vito
Brakhage, Axel A
author_sort Stroe, Maria Cristina
collection PubMed
description Microorganisms produce numerous secondary metabolites (SMs) with various biological activities. Many of their encoding gene clusters are silent under standard laboratory conditions because for their activation they need the ecological context, such as the presence of other microorganisms. The true ecological function of most SMs remains obscure, but understanding of both the activation of silent gene clusters and the ecological function of the produced compounds is of importance to reveal functional interactions in microbiomes. Here, we report the identification of an as-yet uncharacterized silent gene cluster of the fungus Aspergillus fumigatus, which is activated by the bacterium Streptomyces rapamycinicus during the bacterial-fungal interaction. The resulting natural product is the novel fungal metabolite fumigermin, the biosynthesis of which requires the polyketide synthase FgnA. Fumigermin inhibits germination of spores of the inducing S. rapamycinicus, and thus helps the fungus to defend resources in the shared habitat against a bacterial competitor.
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spelling pubmed-70349782020-02-24 Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin Stroe, Maria Cristina Netzker, Tina Scherlach, Kirstin Krüger, Thomas Hertweck, Christian Valiante, Vito Brakhage, Axel A eLife Ecology Microorganisms produce numerous secondary metabolites (SMs) with various biological activities. Many of their encoding gene clusters are silent under standard laboratory conditions because for their activation they need the ecological context, such as the presence of other microorganisms. The true ecological function of most SMs remains obscure, but understanding of both the activation of silent gene clusters and the ecological function of the produced compounds is of importance to reveal functional interactions in microbiomes. Here, we report the identification of an as-yet uncharacterized silent gene cluster of the fungus Aspergillus fumigatus, which is activated by the bacterium Streptomyces rapamycinicus during the bacterial-fungal interaction. The resulting natural product is the novel fungal metabolite fumigermin, the biosynthesis of which requires the polyketide synthase FgnA. Fumigermin inhibits germination of spores of the inducing S. rapamycinicus, and thus helps the fungus to defend resources in the shared habitat against a bacterial competitor. eLife Sciences Publications, Ltd 2020-02-21 /pmc/articles/PMC7034978/ /pubmed/32083553 http://dx.doi.org/10.7554/eLife.52541 Text en © 2020, Stroe et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Ecology
Stroe, Maria Cristina
Netzker, Tina
Scherlach, Kirstin
Krüger, Thomas
Hertweck, Christian
Valiante, Vito
Brakhage, Axel A
Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
title Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
title_full Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
title_fullStr Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
title_full_unstemmed Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
title_short Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
title_sort targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034978/
https://www.ncbi.nlm.nih.gov/pubmed/32083553
http://dx.doi.org/10.7554/eLife.52541
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