Cargando…
Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
Microorganisms produce numerous secondary metabolites (SMs) with various biological activities. Many of their encoding gene clusters are silent under standard laboratory conditions because for their activation they need the ecological context, such as the presence of other microorganisms. The true e...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034978/ https://www.ncbi.nlm.nih.gov/pubmed/32083553 http://dx.doi.org/10.7554/eLife.52541 |
_version_ | 1783499979458871296 |
---|---|
author | Stroe, Maria Cristina Netzker, Tina Scherlach, Kirstin Krüger, Thomas Hertweck, Christian Valiante, Vito Brakhage, Axel A |
author_facet | Stroe, Maria Cristina Netzker, Tina Scherlach, Kirstin Krüger, Thomas Hertweck, Christian Valiante, Vito Brakhage, Axel A |
author_sort | Stroe, Maria Cristina |
collection | PubMed |
description | Microorganisms produce numerous secondary metabolites (SMs) with various biological activities. Many of their encoding gene clusters are silent under standard laboratory conditions because for their activation they need the ecological context, such as the presence of other microorganisms. The true ecological function of most SMs remains obscure, but understanding of both the activation of silent gene clusters and the ecological function of the produced compounds is of importance to reveal functional interactions in microbiomes. Here, we report the identification of an as-yet uncharacterized silent gene cluster of the fungus Aspergillus fumigatus, which is activated by the bacterium Streptomyces rapamycinicus during the bacterial-fungal interaction. The resulting natural product is the novel fungal metabolite fumigermin, the biosynthesis of which requires the polyketide synthase FgnA. Fumigermin inhibits germination of spores of the inducing S. rapamycinicus, and thus helps the fungus to defend resources in the shared habitat against a bacterial competitor. |
format | Online Article Text |
id | pubmed-7034978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-70349782020-02-24 Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin Stroe, Maria Cristina Netzker, Tina Scherlach, Kirstin Krüger, Thomas Hertweck, Christian Valiante, Vito Brakhage, Axel A eLife Ecology Microorganisms produce numerous secondary metabolites (SMs) with various biological activities. Many of their encoding gene clusters are silent under standard laboratory conditions because for their activation they need the ecological context, such as the presence of other microorganisms. The true ecological function of most SMs remains obscure, but understanding of both the activation of silent gene clusters and the ecological function of the produced compounds is of importance to reveal functional interactions in microbiomes. Here, we report the identification of an as-yet uncharacterized silent gene cluster of the fungus Aspergillus fumigatus, which is activated by the bacterium Streptomyces rapamycinicus during the bacterial-fungal interaction. The resulting natural product is the novel fungal metabolite fumigermin, the biosynthesis of which requires the polyketide synthase FgnA. Fumigermin inhibits germination of spores of the inducing S. rapamycinicus, and thus helps the fungus to defend resources in the shared habitat against a bacterial competitor. eLife Sciences Publications, Ltd 2020-02-21 /pmc/articles/PMC7034978/ /pubmed/32083553 http://dx.doi.org/10.7554/eLife.52541 Text en © 2020, Stroe et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Ecology Stroe, Maria Cristina Netzker, Tina Scherlach, Kirstin Krüger, Thomas Hertweck, Christian Valiante, Vito Brakhage, Axel A Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin |
title | Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin |
title_full | Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin |
title_fullStr | Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin |
title_full_unstemmed | Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin |
title_short | Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin |
title_sort | targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin |
topic | Ecology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034978/ https://www.ncbi.nlm.nih.gov/pubmed/32083553 http://dx.doi.org/10.7554/eLife.52541 |
work_keys_str_mv | AT stroemariacristina targetedinductionofasilentfungalgeneclusterencodingthebacteriaspecificgerminationinhibitorfumigermin AT netzkertina targetedinductionofasilentfungalgeneclusterencodingthebacteriaspecificgerminationinhibitorfumigermin AT scherlachkirstin targetedinductionofasilentfungalgeneclusterencodingthebacteriaspecificgerminationinhibitorfumigermin AT krugerthomas targetedinductionofasilentfungalgeneclusterencodingthebacteriaspecificgerminationinhibitorfumigermin AT hertweckchristian targetedinductionofasilentfungalgeneclusterencodingthebacteriaspecificgerminationinhibitorfumigermin AT valiantevito targetedinductionofasilentfungalgeneclusterencodingthebacteriaspecificgerminationinhibitorfumigermin AT brakhageaxela targetedinductionofasilentfungalgeneclusterencodingthebacteriaspecificgerminationinhibitorfumigermin |