Cargando…

A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons

Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here, we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro....

Descripción completa

Detalles Bibliográficos
Autores principales: Abbott, Jeffrey, Ye, Tianyang, Krenek, Keith, Gertner, Rona S., Ban, Steven, Kim, Youbin, Qin, Ling, Wu, Wenxuan, Park, Hongkun, Ham, Donhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035150/
https://www.ncbi.nlm.nih.gov/pubmed/31548592
http://dx.doi.org/10.1038/s41551-019-0455-7
_version_ 1783500016450535424
author Abbott, Jeffrey
Ye, Tianyang
Krenek, Keith
Gertner, Rona S.
Ban, Steven
Kim, Youbin
Qin, Ling
Wu, Wenxuan
Park, Hongkun
Ham, Donhee
author_facet Abbott, Jeffrey
Ye, Tianyang
Krenek, Keith
Gertner, Rona S.
Ban, Steven
Kim, Youbin
Qin, Ling
Wu, Wenxuan
Park, Hongkun
Ham, Donhee
author_sort Abbott, Jeffrey
collection PubMed
description Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here, we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro. The array consists of 4,096 platinum-black electrodes with nanoscale roughness fabricated on top of a silicon chip that monolithically integrates 4,096 microscale amplifiers, configurable into pseudo-current-clamp mode (for concurrent current injection and voltage recording) or into pseudo-voltage-clamp mode (for concurrent voltage application and current recording). We used the array in pseudo-voltage-clamp mode to measure the effects of drugs on ion-channel currents. In pseudo-current-clamp mode, the array recorded intracellular action potentials and post-synaptic potentials from over thousands of neurons. In addition, we mapped over 300 excitatory and inhibitory synaptic connections from over 1,700 neurons that were recorded for 19 mins. This high-throughput intracellular-recording technology could benefit functional connectome mapping, electrophysiological screening, and other functional interrogations of neuronal networks.
format Online
Article
Text
id pubmed-7035150
institution National Center for Biotechnology Information
language English
publishDate 2019
record_format MEDLINE/PubMed
spelling pubmed-70351502020-03-23 A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons Abbott, Jeffrey Ye, Tianyang Krenek, Keith Gertner, Rona S. Ban, Steven Kim, Youbin Qin, Ling Wu, Wenxuan Park, Hongkun Ham, Donhee Nat Biomed Eng Article Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here, we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro. The array consists of 4,096 platinum-black electrodes with nanoscale roughness fabricated on top of a silicon chip that monolithically integrates 4,096 microscale amplifiers, configurable into pseudo-current-clamp mode (for concurrent current injection and voltage recording) or into pseudo-voltage-clamp mode (for concurrent voltage application and current recording). We used the array in pseudo-voltage-clamp mode to measure the effects of drugs on ion-channel currents. In pseudo-current-clamp mode, the array recorded intracellular action potentials and post-synaptic potentials from over thousands of neurons. In addition, we mapped over 300 excitatory and inhibitory synaptic connections from over 1,700 neurons that were recorded for 19 mins. This high-throughput intracellular-recording technology could benefit functional connectome mapping, electrophysiological screening, and other functional interrogations of neuronal networks. 2019-09-23 2020-02 /pmc/articles/PMC7035150/ /pubmed/31548592 http://dx.doi.org/10.1038/s41551-019-0455-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms Reprints and permissions information is available at www.nature.com/reprints. Correspondence and requests for materials should be addressed to H.P. and D.H.
spellingShingle Article
Abbott, Jeffrey
Ye, Tianyang
Krenek, Keith
Gertner, Rona S.
Ban, Steven
Kim, Youbin
Qin, Ling
Wu, Wenxuan
Park, Hongkun
Ham, Donhee
A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
title A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
title_full A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
title_fullStr A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
title_full_unstemmed A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
title_short A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
title_sort nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035150/
https://www.ncbi.nlm.nih.gov/pubmed/31548592
http://dx.doi.org/10.1038/s41551-019-0455-7
work_keys_str_mv AT abbottjeffrey ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT yetianyang ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT krenekkeith ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT gertnerronas ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT bansteven ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT kimyoubin ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT qinling ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT wuwenxuan ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT parkhongkun ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT hamdonhee ananoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT abbottjeffrey nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT yetianyang nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT krenekkeith nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT gertnerronas nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT bansteven nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT kimyoubin nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT qinling nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT wuwenxuan nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT parkhongkun nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons
AT hamdonhee nanoelectrodearrayforobtainingintracellularrecordingsfromthousandsofconnectedneurons