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A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here, we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035150/ https://www.ncbi.nlm.nih.gov/pubmed/31548592 http://dx.doi.org/10.1038/s41551-019-0455-7 |
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author | Abbott, Jeffrey Ye, Tianyang Krenek, Keith Gertner, Rona S. Ban, Steven Kim, Youbin Qin, Ling Wu, Wenxuan Park, Hongkun Ham, Donhee |
author_facet | Abbott, Jeffrey Ye, Tianyang Krenek, Keith Gertner, Rona S. Ban, Steven Kim, Youbin Qin, Ling Wu, Wenxuan Park, Hongkun Ham, Donhee |
author_sort | Abbott, Jeffrey |
collection | PubMed |
description | Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here, we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro. The array consists of 4,096 platinum-black electrodes with nanoscale roughness fabricated on top of a silicon chip that monolithically integrates 4,096 microscale amplifiers, configurable into pseudo-current-clamp mode (for concurrent current injection and voltage recording) or into pseudo-voltage-clamp mode (for concurrent voltage application and current recording). We used the array in pseudo-voltage-clamp mode to measure the effects of drugs on ion-channel currents. In pseudo-current-clamp mode, the array recorded intracellular action potentials and post-synaptic potentials from over thousands of neurons. In addition, we mapped over 300 excitatory and inhibitory synaptic connections from over 1,700 neurons that were recorded for 19 mins. This high-throughput intracellular-recording technology could benefit functional connectome mapping, electrophysiological screening, and other functional interrogations of neuronal networks. |
format | Online Article Text |
id | pubmed-7035150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-70351502020-03-23 A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons Abbott, Jeffrey Ye, Tianyang Krenek, Keith Gertner, Rona S. Ban, Steven Kim, Youbin Qin, Ling Wu, Wenxuan Park, Hongkun Ham, Donhee Nat Biomed Eng Article Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here, we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro. The array consists of 4,096 platinum-black electrodes with nanoscale roughness fabricated on top of a silicon chip that monolithically integrates 4,096 microscale amplifiers, configurable into pseudo-current-clamp mode (for concurrent current injection and voltage recording) or into pseudo-voltage-clamp mode (for concurrent voltage application and current recording). We used the array in pseudo-voltage-clamp mode to measure the effects of drugs on ion-channel currents. In pseudo-current-clamp mode, the array recorded intracellular action potentials and post-synaptic potentials from over thousands of neurons. In addition, we mapped over 300 excitatory and inhibitory synaptic connections from over 1,700 neurons that were recorded for 19 mins. This high-throughput intracellular-recording technology could benefit functional connectome mapping, electrophysiological screening, and other functional interrogations of neuronal networks. 2019-09-23 2020-02 /pmc/articles/PMC7035150/ /pubmed/31548592 http://dx.doi.org/10.1038/s41551-019-0455-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms Reprints and permissions information is available at www.nature.com/reprints. Correspondence and requests for materials should be addressed to H.P. and D.H. |
spellingShingle | Article Abbott, Jeffrey Ye, Tianyang Krenek, Keith Gertner, Rona S. Ban, Steven Kim, Youbin Qin, Ling Wu, Wenxuan Park, Hongkun Ham, Donhee A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons |
title | A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons |
title_full | A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons |
title_fullStr | A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons |
title_full_unstemmed | A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons |
title_short | A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons |
title_sort | nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035150/ https://www.ncbi.nlm.nih.gov/pubmed/31548592 http://dx.doi.org/10.1038/s41551-019-0455-7 |
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