Replacing the eleven native tryptophans by directed evolution produces an active P-glycoprotein with site-specific, non-conservative substitutions

P-glycoprotein (Pgp) pumps an array of hydrophobic compounds out of cells, and has major roles in drug pharmacokinetics and cancer multidrug resistance. Yet, polyspecific drug binding and ATP hydrolysis-driven drug export in Pgp are poorly understood. Fluorescence spectroscopy using tryptophans (Trp...

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Detalles Bibliográficos
Autores principales: Swartz, Douglas J., Singh, Anukriti, Sok, Narong, Thomas, Joshua N., Weber, Joachim, Urbatsch, Ina L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035247/
https://www.ncbi.nlm.nih.gov/pubmed/32081894
http://dx.doi.org/10.1038/s41598-020-59802-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035247/
https://www.ncbi.nlm.nih.gov/pubmed/32081894
http://dx.doi.org/10.1038/s41598-020-59802-w

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