Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking

Salbutamol (SBAL), a kind of short-acting beta 2-adrenergic agonist, has been mainly used to treat bronchial asthma and other allergic airway diseases clinically. In this study, the interaction mechanism between salbutamol and human serum albumin was researched by the multispectral method and molecu...

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Autores principales: Zhao, Ting, Liu, Zihui, Niu, Jingmei, Lv, Baoxing, Xiao, Yuliang, Li, Yuqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035540/
https://www.ncbi.nlm.nih.gov/pubmed/32104676
http://dx.doi.org/10.1155/2020/1693602
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author Zhao, Ting
Liu, Zihui
Niu, Jingmei
Lv, Baoxing
Xiao, Yuliang
Li, Yuqin
author_facet Zhao, Ting
Liu, Zihui
Niu, Jingmei
Lv, Baoxing
Xiao, Yuliang
Li, Yuqin
author_sort Zhao, Ting
collection PubMed
description Salbutamol (SBAL), a kind of short-acting beta 2-adrenergic agonist, has been mainly used to treat bronchial asthma and other allergic airway diseases clinically. In this study, the interaction mechanism between salbutamol and human serum albumin was researched by the multispectral method and molecular docking. The fluorescence intensity of HSA could be regularly enhanced with the increase of SBAL concentration. Both the results of the multispectral method and molecular docking showed that SBAL could bind HSA with van der Waals force and hydrogen bonds. The binding mechanism was further analysed by UV-Vis and synchronous fluorescence spectra. The contents of the secondary structure of free HSA and SBAL-HSA complex were evaluated using CD spectra.
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spelling pubmed-70355402020-02-26 Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking Zhao, Ting Liu, Zihui Niu, Jingmei Lv, Baoxing Xiao, Yuliang Li, Yuqin Biomed Res Int Research Article Salbutamol (SBAL), a kind of short-acting beta 2-adrenergic agonist, has been mainly used to treat bronchial asthma and other allergic airway diseases clinically. In this study, the interaction mechanism between salbutamol and human serum albumin was researched by the multispectral method and molecular docking. The fluorescence intensity of HSA could be regularly enhanced with the increase of SBAL concentration. Both the results of the multispectral method and molecular docking showed that SBAL could bind HSA with van der Waals force and hydrogen bonds. The binding mechanism was further analysed by UV-Vis and synchronous fluorescence spectra. The contents of the secondary structure of free HSA and SBAL-HSA complex were evaluated using CD spectra. Hindawi 2020-02-10 /pmc/articles/PMC7035540/ /pubmed/32104676 http://dx.doi.org/10.1155/2020/1693602 Text en Copyright © 2020 Ting Zhao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Ting
Liu, Zihui
Niu, Jingmei
Lv, Baoxing
Xiao, Yuliang
Li, Yuqin
Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking
title Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking
title_full Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking
title_fullStr Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking
title_full_unstemmed Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking
title_short Investigation of the Interaction Mechanism between Salbutamol and Human Serum Albumin by Multispectroscopic and Molecular Docking
title_sort investigation of the interaction mechanism between salbutamol and human serum albumin by multispectroscopic and molecular docking
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035540/
https://www.ncbi.nlm.nih.gov/pubmed/32104676
http://dx.doi.org/10.1155/2020/1693602
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