Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of Toxocara canis for External Quality Assessment Schemes

BACKGROUND: External quality assessment (EQA) provides evidence of reliable, accurate, and precise results for customers using the diagnostic test for Toxocara canis. Objective. To establish a procedure for producing standard Toxocara canis serum samples for serodiagnostic testing in EQA. METHODS: The collected serum samples to contain anti-Toxocara canis serum samples for serodiagnostic testing in EQA. F-test and a T-test were applied to evaluate their homogeneity and stability. RESULTS: Among eleven samples positive by ELISA, ten of them were confirmed via Western blotting by positive reaction with 5 specific Toxocara canis serum samples for serodiagnostic testing in EQA. Toxocara canis serum samples for serodiagnostic testing in EQA. p > 0.05). Samples produced by both methods were stable for 7 days at 30°C (p > 0.05). Samples produced by both methods were stable for 7 days at 30°C ( CONCLUSION: Specific serodiagnosis samples of anti-Toxocara canis antibodies for EQA could be produced that possessed homogeneity and stability lasting for 3 months and 6 months by the freeze-drying and freezing methods, respectively. At 30°C, the samples produced by both methods were stable for 7 days, suitable for delivery to remote laboratories.Toxocara canis serum samples for serodiagnostic testing in EQA.