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Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli

Obese mice exhibited more lymphocytes in the bronchoalveolar lavage fluid and milder lung injury after Escherichia coli (E. coli) infection. However, it remained unclear whether the spleen contributed to the effect of obese mice with infection. The study was purposed to reveal the histopathological...

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Autores principales: Gu, Xuchu, Ma, Zhiyu, Fang, Jing, Cai, Dongjie, Zuo, Zhicai, Liang, Shuang, Cui, Hengmin, Deng, Junliang, Ma, Xiaoping, Ren, Zhihua, Geng, Yi, Zhang, Ming, Ye, Gang, Xie, Yue, Gou, Liping, Hu, Yanchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036121/
https://www.ncbi.nlm.nih.gov/pubmed/32104715
http://dx.doi.org/10.1155/2020/5948256
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author Gu, Xuchu
Ma, Zhiyu
Fang, Jing
Cai, Dongjie
Zuo, Zhicai
Liang, Shuang
Cui, Hengmin
Deng, Junliang
Ma, Xiaoping
Ren, Zhihua
Geng, Yi
Zhang, Ming
Ye, Gang
Xie, Yue
Gou, Liping
Hu, Yanchun
author_facet Gu, Xuchu
Ma, Zhiyu
Fang, Jing
Cai, Dongjie
Zuo, Zhicai
Liang, Shuang
Cui, Hengmin
Deng, Junliang
Ma, Xiaoping
Ren, Zhihua
Geng, Yi
Zhang, Ming
Ye, Gang
Xie, Yue
Gou, Liping
Hu, Yanchun
author_sort Gu, Xuchu
collection PubMed
description Obese mice exhibited more lymphocytes in the bronchoalveolar lavage fluid and milder lung injury after Escherichia coli (E. coli) infection. However, it remained unclear whether the spleen contributed to the effect of obese mice with infection. The study was purposed to reveal the histopathological changes of the spleen caused by oxidative stress and inflammation in diet-induced obesity (DIO) mice challenged by Escherichia coli. After infection, the spleen tissues were obtained in normal and DIO mice at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. Results revealed that DIO mice have higher contents of resistin, TNF-α, IL-6, and IL-1β in the spleen than normal mice and lower concentrations of GSH-Px, SOD, and CAT and higher MDA than normal mice. After an intranasal drip of E. coli, the activities of GSH-Px, SOD, and CAT in the DIO mice were elevated and the content of MDA declined. The activities of SOD and CAT in the normal mice declined, and the content of MDA was elevated. Moreover, the contents of TNF-α, IL-6, and IL-1β in the spleen declined in DIO mice at 24 and 72 h, although the contents of leptin, resistin, TNF-α, IL-6, and IL-1β were elevated at 12 h. The contents of resistin, TNF-α, IL-6, and IL-1β were elevated in normal mice at 12 and 24 h. Those results indicated that obesity elevated splenic oxidation and inflammatory levels, but it enhanced antioxidant capacity and reduced cytokine levels of the spleen in mice to resist splenic injury after an intranasal drip of E. coli.
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spelling pubmed-70361212020-02-26 Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli Gu, Xuchu Ma, Zhiyu Fang, Jing Cai, Dongjie Zuo, Zhicai Liang, Shuang Cui, Hengmin Deng, Junliang Ma, Xiaoping Ren, Zhihua Geng, Yi Zhang, Ming Ye, Gang Xie, Yue Gou, Liping Hu, Yanchun J Immunol Res Research Article Obese mice exhibited more lymphocytes in the bronchoalveolar lavage fluid and milder lung injury after Escherichia coli (E. coli) infection. However, it remained unclear whether the spleen contributed to the effect of obese mice with infection. The study was purposed to reveal the histopathological changes of the spleen caused by oxidative stress and inflammation in diet-induced obesity (DIO) mice challenged by Escherichia coli. After infection, the spleen tissues were obtained in normal and DIO mice at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. Results revealed that DIO mice have higher contents of resistin, TNF-α, IL-6, and IL-1β in the spleen than normal mice and lower concentrations of GSH-Px, SOD, and CAT and higher MDA than normal mice. After an intranasal drip of E. coli, the activities of GSH-Px, SOD, and CAT in the DIO mice were elevated and the content of MDA declined. The activities of SOD and CAT in the normal mice declined, and the content of MDA was elevated. Moreover, the contents of TNF-α, IL-6, and IL-1β in the spleen declined in DIO mice at 24 and 72 h, although the contents of leptin, resistin, TNF-α, IL-6, and IL-1β were elevated at 12 h. The contents of resistin, TNF-α, IL-6, and IL-1β were elevated in normal mice at 12 and 24 h. Those results indicated that obesity elevated splenic oxidation and inflammatory levels, but it enhanced antioxidant capacity and reduced cytokine levels of the spleen in mice to resist splenic injury after an intranasal drip of E. coli. Hindawi 2020-02-11 /pmc/articles/PMC7036121/ /pubmed/32104715 http://dx.doi.org/10.1155/2020/5948256 Text en Copyright © 2020 Xuchu Gu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gu, Xuchu
Ma, Zhiyu
Fang, Jing
Cai, Dongjie
Zuo, Zhicai
Liang, Shuang
Cui, Hengmin
Deng, Junliang
Ma, Xiaoping
Ren, Zhihua
Geng, Yi
Zhang, Ming
Ye, Gang
Xie, Yue
Gou, Liping
Hu, Yanchun
Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli
title Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli
title_full Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli
title_fullStr Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli
title_full_unstemmed Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli
title_short Obesity Enhances Antioxidant Capacity and Reduces Cytokine Levels of the Spleen in Mice to Resist Splenic Injury Challenged by Escherichia coli
title_sort obesity enhances antioxidant capacity and reduces cytokine levels of the spleen in mice to resist splenic injury challenged by escherichia coli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036121/
https://www.ncbi.nlm.nih.gov/pubmed/32104715
http://dx.doi.org/10.1155/2020/5948256
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