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Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness

Salicylic acid (SA), an essential secondary messenger for plant defence responses, plays a role in maintaining a balance (trade‐off) between plant growth and resistance induction, but the detailed mechanism has not been explored. Because the SA mimic benzothiadiazole (BTH) is a more stable inducer o...

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Autores principales: Kobayashi, Yudai, Fukuzawa, Noriho, Hyodo, Ayaka, Kim, Hangil, Mashiyama, Shota, Ogihara, Tsuyoshi, Yoshioka, Hirofumi, Matsuura, Hideyuki, Masuta, Chikara, Matsumura, Takeshi, Takeshita, Minoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036366/
https://www.ncbi.nlm.nih.gov/pubmed/31965700
http://dx.doi.org/10.1111/mpp.12906
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author Kobayashi, Yudai
Fukuzawa, Noriho
Hyodo, Ayaka
Kim, Hangil
Mashiyama, Shota
Ogihara, Tsuyoshi
Yoshioka, Hirofumi
Matsuura, Hideyuki
Masuta, Chikara
Matsumura, Takeshi
Takeshita, Minoru
author_facet Kobayashi, Yudai
Fukuzawa, Noriho
Hyodo, Ayaka
Kim, Hangil
Mashiyama, Shota
Ogihara, Tsuyoshi
Yoshioka, Hirofumi
Matsuura, Hideyuki
Masuta, Chikara
Matsumura, Takeshi
Takeshita, Minoru
author_sort Kobayashi, Yudai
collection PubMed
description Salicylic acid (SA), an essential secondary messenger for plant defence responses, plays a role in maintaining a balance (trade‐off) between plant growth and resistance induction, but the detailed mechanism has not been explored. Because the SA mimic benzothiadiazole (BTH) is a more stable inducer of plant defence than SA after exogenous application, we analysed expression profiles of defence genes after BTH treatment to better understand SA‐mediated immune induction. Transcript levels of the salicylic acid glucosyltransferase (SAGT) gene were significantly lower in BTH‐treated Nicotiana tabacum (Nt) plants than in SA‐treated Nt control plants, suggesting that SAGT may play an important role in SA‐related host defence responses. Treatment with BTH followed by SA suppressed SAGT transcription, indicating that the inhibitory effect of BTH is not reversible. In addition, in BTH‐treated Nt and Nicotiana benthamiana (Nb) plants, an early high accumulation of SA and SA 2‐O‐β‐d‐glucoside was only transient compared to the control. This observation agreed well with the finding that SAGT‐overexpressing (OE) Nb lines contained less SA and jasmonic acid (JA) than in the Nb plants. When inoculated with a virus, the OE Nb plants showed more severe symptoms and accumulated higher levels of virus, while resistance increased in SAGT‐silenced (IR) Nb plants. In addition, the IR plants restricted bacterial spread to the inoculated leaves. After the BTH treatment, OE Nb plants were slightly larger than the Nb plants. These results together indicate that SAGT has a pivotal role in the balance between plant growth and SA/JA‐mediated defence for optimum plant fitness.
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spelling pubmed-70363662020-02-26 Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness Kobayashi, Yudai Fukuzawa, Noriho Hyodo, Ayaka Kim, Hangil Mashiyama, Shota Ogihara, Tsuyoshi Yoshioka, Hirofumi Matsuura, Hideyuki Masuta, Chikara Matsumura, Takeshi Takeshita, Minoru Mol Plant Pathol Original Articles Salicylic acid (SA), an essential secondary messenger for plant defence responses, plays a role in maintaining a balance (trade‐off) between plant growth and resistance induction, but the detailed mechanism has not been explored. Because the SA mimic benzothiadiazole (BTH) is a more stable inducer of plant defence than SA after exogenous application, we analysed expression profiles of defence genes after BTH treatment to better understand SA‐mediated immune induction. Transcript levels of the salicylic acid glucosyltransferase (SAGT) gene were significantly lower in BTH‐treated Nicotiana tabacum (Nt) plants than in SA‐treated Nt control plants, suggesting that SAGT may play an important role in SA‐related host defence responses. Treatment with BTH followed by SA suppressed SAGT transcription, indicating that the inhibitory effect of BTH is not reversible. In addition, in BTH‐treated Nt and Nicotiana benthamiana (Nb) plants, an early high accumulation of SA and SA 2‐O‐β‐d‐glucoside was only transient compared to the control. This observation agreed well with the finding that SAGT‐overexpressing (OE) Nb lines contained less SA and jasmonic acid (JA) than in the Nb plants. When inoculated with a virus, the OE Nb plants showed more severe symptoms and accumulated higher levels of virus, while resistance increased in SAGT‐silenced (IR) Nb plants. In addition, the IR plants restricted bacterial spread to the inoculated leaves. After the BTH treatment, OE Nb plants were slightly larger than the Nb plants. These results together indicate that SAGT has a pivotal role in the balance between plant growth and SA/JA‐mediated defence for optimum plant fitness. John Wiley and Sons Inc. 2020-01-21 /pmc/articles/PMC7036366/ /pubmed/31965700 http://dx.doi.org/10.1111/mpp.12906 Text en © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Kobayashi, Yudai
Fukuzawa, Noriho
Hyodo, Ayaka
Kim, Hangil
Mashiyama, Shota
Ogihara, Tsuyoshi
Yoshioka, Hirofumi
Matsuura, Hideyuki
Masuta, Chikara
Matsumura, Takeshi
Takeshita, Minoru
Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness
title Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness
title_full Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness
title_fullStr Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness
title_full_unstemmed Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness
title_short Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness
title_sort role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036366/
https://www.ncbi.nlm.nih.gov/pubmed/31965700
http://dx.doi.org/10.1111/mpp.12906
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