Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11

BACKGROUND: Pancreatic cancer (PC) is a highly lethal malignancy worldwide. Our previous study indicated that overexpression of USP34 could promote tumor growth in PC cells. Therefore, this study aimed to further investigate the role of USP34 during the tumorigenesis of PC. METHODS: The level of USP...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Changjie, Xia, Jing, Gu, Zhiwei, Meng, Yunpeng, Gao, Dekang, Wei, Shaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036619/
https://www.ncbi.nlm.nih.gov/pubmed/32110045
http://dx.doi.org/10.2147/OTT.S228857
_version_ 1783500248864260096
author Lin, Changjie
Xia, Jing
Gu, Zhiwei
Meng, Yunpeng
Gao, Dekang
Wei, Shaohua
author_facet Lin, Changjie
Xia, Jing
Gu, Zhiwei
Meng, Yunpeng
Gao, Dekang
Wei, Shaohua
author_sort Lin, Changjie
collection PubMed
description BACKGROUND: Pancreatic cancer (PC) is a highly lethal malignancy worldwide. Our previous study indicated that overexpression of USP34 could promote tumor growth in PC cells. Therefore, this study aimed to further investigate the role of USP34 during the tumorigenesis of PC. METHODS: The level of USP34 in PANC-1 and MiaPaCa-2 cells transfected with USP34-shRNAs was detected by RT-qPCR. Moreover, transwell migration and Annexin V/PI analysis were conducted to detect cell migration and apoptosis, respectively. RESULTS: In this study, downregulation of USP34 markedly inhibited proliferation and migration, and induced apoptosis in PANC-1 cells. Moreover, silencing of USP34 obviously downregulated the levels of PRR11 and p-p38 in PANC-1 cells. An in vivo study in nude mice bearing PANC-1 cell xenografts confirmed these results. CONCLUSION: Downregulation of USP34 could inhibit proliferation and migration in PANC-1 cells via inhibiting PRR11, and inactivating p38 MAPK signaling. Therefore, USP34 might be a potential therapeutic target for the treatment of PC.
format Online
Article
Text
id pubmed-7036619
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-70366192020-02-27 Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11 Lin, Changjie Xia, Jing Gu, Zhiwei Meng, Yunpeng Gao, Dekang Wei, Shaohua Onco Targets Ther Original Research BACKGROUND: Pancreatic cancer (PC) is a highly lethal malignancy worldwide. Our previous study indicated that overexpression of USP34 could promote tumor growth in PC cells. Therefore, this study aimed to further investigate the role of USP34 during the tumorigenesis of PC. METHODS: The level of USP34 in PANC-1 and MiaPaCa-2 cells transfected with USP34-shRNAs was detected by RT-qPCR. Moreover, transwell migration and Annexin V/PI analysis were conducted to detect cell migration and apoptosis, respectively. RESULTS: In this study, downregulation of USP34 markedly inhibited proliferation and migration, and induced apoptosis in PANC-1 cells. Moreover, silencing of USP34 obviously downregulated the levels of PRR11 and p-p38 in PANC-1 cells. An in vivo study in nude mice bearing PANC-1 cell xenografts confirmed these results. CONCLUSION: Downregulation of USP34 could inhibit proliferation and migration in PANC-1 cells via inhibiting PRR11, and inactivating p38 MAPK signaling. Therefore, USP34 might be a potential therapeutic target for the treatment of PC. Dove 2020-02-18 /pmc/articles/PMC7036619/ /pubmed/32110045 http://dx.doi.org/10.2147/OTT.S228857 Text en © 2020 Lin et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Lin, Changjie
Xia, Jing
Gu, Zhiwei
Meng, Yunpeng
Gao, Dekang
Wei, Shaohua
Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11
title Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11
title_full Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11
title_fullStr Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11
title_full_unstemmed Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11
title_short Downregulation of USP34 Inhibits the Growth and Migration of Pancreatic Cancer Cells via Inhibiting the PRR11
title_sort downregulation of usp34 inhibits the growth and migration of pancreatic cancer cells via inhibiting the prr11
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036619/
https://www.ncbi.nlm.nih.gov/pubmed/32110045
http://dx.doi.org/10.2147/OTT.S228857
work_keys_str_mv AT linchangjie downregulationofusp34inhibitsthegrowthandmigrationofpancreaticcancercellsviainhibitingtheprr11
AT xiajing downregulationofusp34inhibitsthegrowthandmigrationofpancreaticcancercellsviainhibitingtheprr11
AT guzhiwei downregulationofusp34inhibitsthegrowthandmigrationofpancreaticcancercellsviainhibitingtheprr11
AT mengyunpeng downregulationofusp34inhibitsthegrowthandmigrationofpancreaticcancercellsviainhibitingtheprr11
AT gaodekang downregulationofusp34inhibitsthegrowthandmigrationofpancreaticcancercellsviainhibitingtheprr11
AT weishaohua downregulationofusp34inhibitsthegrowthandmigrationofpancreaticcancercellsviainhibitingtheprr11

Ejemplares similares