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Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation

To clarify whether flavonoids and phenols in Allium mongolicum Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of A. mongolicum by using various chromatographic and spectrophotometric met...

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Autores principales: Dong, Yongzhe, Ruan, Jingya, Ding, Zhijuan, Zhao, Wei, Hao, Mimi, Zhang, Ying, Jiang, Hongyu, Zhang, Yi, Wang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036834/
https://www.ncbi.nlm.nih.gov/pubmed/32013151
http://dx.doi.org/10.3390/molecules25030577
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author Dong, Yongzhe
Ruan, Jingya
Ding, Zhijuan
Zhao, Wei
Hao, Mimi
Zhang, Ying
Jiang, Hongyu
Zhang, Yi
Wang, Tao
author_facet Dong, Yongzhe
Ruan, Jingya
Ding, Zhijuan
Zhao, Wei
Hao, Mimi
Zhang, Ying
Jiang, Hongyu
Zhang, Yi
Wang, Tao
author_sort Dong, Yongzhe
collection PubMed
description To clarify whether flavonoids and phenols in Allium mongolicum Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of A. mongolicum by using various chromatographic and spectrophotometric methods, a bioassay on motility of mouse isolated intestine tissue, as well as qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis. As a result, 31 flavonoids and phenolic acids were obtained and identified, including six new flavonoid glycosides, mongoflavonosides A(1) (1), A(2) (2), A(3) (3), A(4) (4), B(1) (5), B(2) (6), and four new phenolic acid glycosides, mongophenosides A(1) (7), A(2) (8), A(3) (9), B (10). Among them, eleven flavonoids and three phenolic acids showed significant increase in the height of mouse small intestinal muscle. It was a first systematic bioactive constituents’ study for A. mongolicum on gastrointestinal tract. Furthermore, according to the retention time (t(R)) and the exact mass-to-charge ratio (m/z), thirty-one compounds were unambiguously identified by comparing to the standard references by using LC-MS. Then, on the basis of generalized rules of MS/MS fragmentation pattern, chromatographic behaviors, as well as biosynthetic laws of the 31 isolates, five flavonoid glycosides and one phenolic acid glycoside were tentatively speculated. On the basis of the study, a fast analysis method for flavonoids and phenolic acids in A. mongolicum was established.
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spelling pubmed-70368342020-03-11 Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation Dong, Yongzhe Ruan, Jingya Ding, Zhijuan Zhao, Wei Hao, Mimi Zhang, Ying Jiang, Hongyu Zhang, Yi Wang, Tao Molecules Article To clarify whether flavonoids and phenols in Allium mongolicum Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of A. mongolicum by using various chromatographic and spectrophotometric methods, a bioassay on motility of mouse isolated intestine tissue, as well as qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis. As a result, 31 flavonoids and phenolic acids were obtained and identified, including six new flavonoid glycosides, mongoflavonosides A(1) (1), A(2) (2), A(3) (3), A(4) (4), B(1) (5), B(2) (6), and four new phenolic acid glycosides, mongophenosides A(1) (7), A(2) (8), A(3) (9), B (10). Among them, eleven flavonoids and three phenolic acids showed significant increase in the height of mouse small intestinal muscle. It was a first systematic bioactive constituents’ study for A. mongolicum on gastrointestinal tract. Furthermore, according to the retention time (t(R)) and the exact mass-to-charge ratio (m/z), thirty-one compounds were unambiguously identified by comparing to the standard references by using LC-MS. Then, on the basis of generalized rules of MS/MS fragmentation pattern, chromatographic behaviors, as well as biosynthetic laws of the 31 isolates, five flavonoid glycosides and one phenolic acid glycoside were tentatively speculated. On the basis of the study, a fast analysis method for flavonoids and phenolic acids in A. mongolicum was established. MDPI 2020-01-29 /pmc/articles/PMC7036834/ /pubmed/32013151 http://dx.doi.org/10.3390/molecules25030577 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dong, Yongzhe
Ruan, Jingya
Ding, Zhijuan
Zhao, Wei
Hao, Mimi
Zhang, Ying
Jiang, Hongyu
Zhang, Yi
Wang, Tao
Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation
title Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation
title_full Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation
title_fullStr Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation
title_full_unstemmed Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation
title_short Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation
title_sort phytochemistry and comprehensive chemical profiling study of flavonoids and phenolic acids in the aerial parts of allium mongolicum regel and their intestinal motility evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036834/
https://www.ncbi.nlm.nih.gov/pubmed/32013151
http://dx.doi.org/10.3390/molecules25030577
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