Cargando…

Expanding the CRISPR Toolbox in P. patens Using SpCas9-NG Variant and Application for Gene and Base Editing in Solanaceae Crops

Genome editing has become a major tool for both functional studies and plant breeding in several species. Besides generating knockouts through the classical CRISPR-Cas9 system, recent development of CRISPR base editing holds great and exciting opportunities for the production of gain-of-function mut...

Descripción completa

Detalles Bibliográficos
Autores principales:	Veillet, Florian, Perrot, Laura, Guyon-Debast, Anouchka, Kermarrec, Marie-Paule, Chauvin, Laura, Chauvin, Jean-Eric, Gallois, Jean-Luc, Mazier, Marianne, Nogué, Fabien
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036883/ https://www.ncbi.nlm.nih.gov/pubmed/32033083 http://dx.doi.org/10.3390/ijms21031024

Ejemplares similares

Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor
por: Veillet, Florian, et al.
Publicado: (2019)

A blueprint for gene function analysis through Base Editing in the model plant Physcomitrium (Physcomitrella) patens
por: Guyon‐Debast, Anouchka, et al.
Publicado: (2021)

CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato
por: Veillet, Florian, et al.
Publicado: (2020)

Improved prime editing allows for routine predictable gene editing in Physcomitrium patens
por: Perroud, Pierre-François, et al.
Publicado: (2023)

The XPF-ERCC1 Complex Is Essential for Genome Stability and Is Involved in the Mechanism of Gene Targeting in Physcomitrella patens
por: Guyon-Debast, Anouchka, et al.
Publicado: (2019)

Simple and Efficient Targeting of Multiple Genes Through CRISPR-Cas9 in Physcomitrella patens
por: Lopez-Obando, Mauricio, et al.
Publicado: (2016)

Mimicking natural polymorphism in eIF4E by CRISPR‐Cas9 base editing is associated with resistance to potyviruses
por: Bastet, Anna, et al.
Publicado: (2019)

CRISPR‐Cas9‐mediated efficient directed mutagenesis and RAD51‐dependent and RAD51‐independent gene targeting in the moss Physcomitrella patens
por: Collonnier, Cécile, et al.
Publicado: (2016)

A SNP associated with alternative splicing of RPT5b causes unequal redundancy between RPT5a and RPT5b among Arabidopsis thaliana natural variation
por: Guyon-Debast, Anouchka, et al.
Publicado: (2010)

An iterative gene‐editing strategy broadens eIF4E1 genetic diversity in Solanum lycopersicum and generates resistance to multiple potyvirus isolates
por: Kuroiwa, Kyoka, et al.
Publicado: (2023)

Generation of genetically modified mice using SpCas9-NG engineered nuclease
por: Fujii, Wataru, et al.
Publicado: (2019)

Genome-wide specificity of plant genome editing by both CRISPR–Cas9 and TALEN
por: Bessoltane, Nadia, et al.
Publicado: (2022)

The DFR locus: A smart landing pad for targeted transgene insertion in tomato
por: Danilo, Benoit, et al.
Publicado: (2018)

Precise CAG repeat contraction in a Huntington’s Disease mouse model is enabled by gene editing with SpCas9-NG
por: Oura, Seiya, et al.
Publicado: (2021)

MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens
por: Trouiller, Bénédicte, et al.
Publicado: (2006)

Engineered xCas9 and SpCas9‐NG variants broaden PAM recognition sites to generate mutations in Arabidopsis plants
por: Ge, Zengxiang, et al.
Publicado: (2019)

Multiplex CRISPR-Cas9 mutagenesis of the phytochrome gene family in Physcomitrium (Physcomitrella) patens
por: Trogu, Silvia, et al.
Publicado: (2020)

In Silico and Biochemical Analysis of Physcomitrella patens Photosynthetic Antenna: Identification of Subunits which Evolved upon Land Adaptation
por: Alboresi, Alessandro, et al.
Publicado: (2008)

Different Families of Retrotransposons and DNA Transposons Are Actively Transcribed and May Have Transposed Recently in Physcomitrium (Physcomitrella) patens
por: Vendrell-Mir, Pol, et al.
Publicado: (2020)

CHASE domain-containing receptors play an essential role in the cytokinin response of the moss Physcomitrella patens
por: von Schwartzenberg, Klaus, et al.
Publicado: (2016)

MRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens
por: Kamisugi, Yasuko, et al.
Publicado: (2012)

Production of Pharmaceutical Proteins in Solanaceae Food Crops
por: Rigano, Maria Manuela, et al.
Publicado: (2013)

Solanaceae /
por: Nee, Michael
Publicado: (1986)

CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato
por: Lukan, Tjaša, et al.
Publicado: (2022)

Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology
por: Sakai, Kaori, et al.
Publicado: (2019)

In Planta Processing of the SpCas9–gRNA Complex
por: Mikami, Masafumi, et al.
Publicado: (2017)

Unravelling 3D growth in the moss Physcomitrium patens
por: Moody, Laura A.
Publicado: (2022)

Antifungal Activity of Beauveria bassiana Endophyte against Botrytis cinerea in Two Solanaceae Crops
por: Barra-Bucarei, Lorena, et al.
Publicado: (2019)

Structural insights into a high fidelity variant of SpCas9
por: Guo, Minghui, et al.
Publicado: (2019)

Probing the stability of the SpCas9–DNA complex after cleavage
por: Aldag, Pierre, et al.
Publicado: (2021)

On the Proton-Bound Noble Gas Dimers (Ng-H-Ng)(+) and (Ng-H-Ng’)(+) (Ng, Ng’ = He-Xe): Relationships between Structure, Stability, and Bonding Character
por: Borocci, Stefano, et al.
Publicado: (2021)

Efficient correction of Duchenne muscular dystrophy mutations by SpCas9 and dual gRNAs
por: Xiang, Xi, et al.
Publicado: (2021)

RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella
por: Charlot, Florence, et al.
Publicado: (2014)

Minimal PAM specificity of a highly similar SpCas9 ortholog
por: Chatterjee, Pranam, et al.
Publicado: (2018)

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
por: Hart, Traver, et al.
Publicado: (2017)

Continuous evolution of SpCas9 variants compatible with non-G PAMs
por: Miller, Shannon M., et al.
Publicado: (2020)

Engineered dual selection for directed evolution of SpCas9 PAM specificity
por: Goldberg, Gregory W., et al.
Publicado: (2021)

A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets
por: Kulcsár, Péter István, et al.
Publicado: (2023)

A Review of Bioinsecticidal Activity of Solanaceae Alkaloids
por: Chowański, Szymon, et al.
Publicado: (2016)

Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs
por: Huszár, Krisztina, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_0ikljf4a76k8j7r2db9eooa58f