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Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis

In sepsis, platelets may become activated via toll-like receptors (TLRs), causing microvascular thrombosis. Megakaryocytes (MKs) also express these receptors; thus, severe infection may modulate thrombopoiesis. To explore the relevance of altered miRNAs in platelet activation upon sepsis, we first i...

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Autores principales: Szilágyi, Bernadett, Fejes, Zsolt, Póliska, Szilárd, Pócsi, Marianna, Czimmerer, Zsolt, Patsalos, Andreas, Fenyvesi, Ferenc, Rusznyák, Ágnes, Nagy, György, Kerekes, György, Berhés, Mariann, Szűcs, Ildikó, Kunapuli, Satya P., Kappelmayer, János, Nagy, Béla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036890/
https://www.ncbi.nlm.nih.gov/pubmed/32013235
http://dx.doi.org/10.3390/ijms21030866
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author Szilágyi, Bernadett
Fejes, Zsolt
Póliska, Szilárd
Pócsi, Marianna
Czimmerer, Zsolt
Patsalos, Andreas
Fenyvesi, Ferenc
Rusznyák, Ágnes
Nagy, György
Kerekes, György
Berhés, Mariann
Szűcs, Ildikó
Kunapuli, Satya P.
Kappelmayer, János
Nagy, Béla
author_facet Szilágyi, Bernadett
Fejes, Zsolt
Póliska, Szilárd
Pócsi, Marianna
Czimmerer, Zsolt
Patsalos, Andreas
Fenyvesi, Ferenc
Rusznyák, Ágnes
Nagy, György
Kerekes, György
Berhés, Mariann
Szűcs, Ildikó
Kunapuli, Satya P.
Kappelmayer, János
Nagy, Béla
author_sort Szilágyi, Bernadett
collection PubMed
description In sepsis, platelets may become activated via toll-like receptors (TLRs), causing microvascular thrombosis. Megakaryocytes (MKs) also express these receptors; thus, severe infection may modulate thrombopoiesis. To explore the relevance of altered miRNAs in platelet activation upon sepsis, we first investigated sepsis-induced miRNA expression in platelets of septic patients. The effect of abnormal Dicer level on miRNA expression was also evaluated. miRNAs were profiled in septic vs. normal platelets using TaqMan Open Array. We validated platelet miR-26b with its target SELP (P-selectin) mRNA levels and correlated them with clinical outcomes. The impact of sepsis on MK transcriptome was analyzed in MEG-01 cells after lipopolysaccharide (LPS) treatment by RNA-seq. Sepsis-reduced miR-26b was further studied using Dicer1 siRNA and calpain inhibition in MEG-01 cells. Out of 390 platelet miRNAs detected, there were 121 significantly decreased, and 61 upregulated in sepsis vs. controls. Septic platelets showed attenuated miR-26b, which were associated with disease severity and mortality. SELP mRNA level was elevated in sepsis, especially in platelets with increased mean platelet volume, causing higher P-selectin expression. Downregulation of Dicer1 generated lower miR-26b with higher SELP mRNA, while calpeptin restored miR-26b in MEG-01 cells. In conclusion, decreased miR-26b in MKs and platelets contributes to an increased level of platelet activation status in sepsis.
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spelling pubmed-70368902020-03-11 Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis Szilágyi, Bernadett Fejes, Zsolt Póliska, Szilárd Pócsi, Marianna Czimmerer, Zsolt Patsalos, Andreas Fenyvesi, Ferenc Rusznyák, Ágnes Nagy, György Kerekes, György Berhés, Mariann Szűcs, Ildikó Kunapuli, Satya P. Kappelmayer, János Nagy, Béla Int J Mol Sci Article In sepsis, platelets may become activated via toll-like receptors (TLRs), causing microvascular thrombosis. Megakaryocytes (MKs) also express these receptors; thus, severe infection may modulate thrombopoiesis. To explore the relevance of altered miRNAs in platelet activation upon sepsis, we first investigated sepsis-induced miRNA expression in platelets of septic patients. The effect of abnormal Dicer level on miRNA expression was also evaluated. miRNAs were profiled in septic vs. normal platelets using TaqMan Open Array. We validated platelet miR-26b with its target SELP (P-selectin) mRNA levels and correlated them with clinical outcomes. The impact of sepsis on MK transcriptome was analyzed in MEG-01 cells after lipopolysaccharide (LPS) treatment by RNA-seq. Sepsis-reduced miR-26b was further studied using Dicer1 siRNA and calpain inhibition in MEG-01 cells. Out of 390 platelet miRNAs detected, there were 121 significantly decreased, and 61 upregulated in sepsis vs. controls. Septic platelets showed attenuated miR-26b, which were associated with disease severity and mortality. SELP mRNA level was elevated in sepsis, especially in platelets with increased mean platelet volume, causing higher P-selectin expression. Downregulation of Dicer1 generated lower miR-26b with higher SELP mRNA, while calpeptin restored miR-26b in MEG-01 cells. In conclusion, decreased miR-26b in MKs and platelets contributes to an increased level of platelet activation status in sepsis. MDPI 2020-01-29 /pmc/articles/PMC7036890/ /pubmed/32013235 http://dx.doi.org/10.3390/ijms21030866 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szilágyi, Bernadett
Fejes, Zsolt
Póliska, Szilárd
Pócsi, Marianna
Czimmerer, Zsolt
Patsalos, Andreas
Fenyvesi, Ferenc
Rusznyák, Ágnes
Nagy, György
Kerekes, György
Berhés, Mariann
Szűcs, Ildikó
Kunapuli, Satya P.
Kappelmayer, János
Nagy, Béla
Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis
title Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis
title_full Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis
title_fullStr Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis
title_full_unstemmed Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis
title_short Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis
title_sort reduced mir-26b expression in megakaryocytes and platelets contributes to elevated level of platelet activation status in sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036890/
https://www.ncbi.nlm.nih.gov/pubmed/32013235
http://dx.doi.org/10.3390/ijms21030866
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