Cargando…
Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm(5)s(2)U), synthetized b...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036906/ https://www.ncbi.nlm.nih.gov/pubmed/32023806 http://dx.doi.org/10.3390/ijms21030925 |
_version_ | 1783500302829223936 |
---|---|
author | Rojas-Benítez, Diego L. Allende, Miguel |
author_facet | Rojas-Benítez, Diego L. Allende, Miguel |
author_sort | Rojas-Benítez, Diego |
collection | PubMed |
description | Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm(5)s(2)U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm(5)s(2)U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism. |
format | Online Article Text |
id | pubmed-7036906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70369062020-03-11 Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development Rojas-Benítez, Diego L. Allende, Miguel Int J Mol Sci Article Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm(5)s(2)U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm(5)s(2)U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism. MDPI 2020-01-31 /pmc/articles/PMC7036906/ /pubmed/32023806 http://dx.doi.org/10.3390/ijms21030925 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rojas-Benítez, Diego L. Allende, Miguel Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development |
title | Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development |
title_full | Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development |
title_fullStr | Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development |
title_full_unstemmed | Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development |
title_short | Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development |
title_sort | elongator subunit 3 (elp3) is required for zebrafish trunk development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036906/ https://www.ncbi.nlm.nih.gov/pubmed/32023806 http://dx.doi.org/10.3390/ijms21030925 |
work_keys_str_mv | AT rojasbenitezdiego elongatorsubunit3elp3isrequiredforzebrafishtrunkdevelopment AT lallendemiguel elongatorsubunit3elp3isrequiredforzebrafishtrunkdevelopment |