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Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development

Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm(5)s(2)U), synthetized b...

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Autores principales: Rojas-Benítez, Diego, L. Allende, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036906/
https://www.ncbi.nlm.nih.gov/pubmed/32023806
http://dx.doi.org/10.3390/ijms21030925
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author Rojas-Benítez, Diego
L. Allende, Miguel
author_facet Rojas-Benítez, Diego
L. Allende, Miguel
author_sort Rojas-Benítez, Diego
collection PubMed
description Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm(5)s(2)U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm(5)s(2)U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism.
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spelling pubmed-70369062020-03-11 Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development Rojas-Benítez, Diego L. Allende, Miguel Int J Mol Sci Article Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm(5)s(2)U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm(5)s(2)U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism. MDPI 2020-01-31 /pmc/articles/PMC7036906/ /pubmed/32023806 http://dx.doi.org/10.3390/ijms21030925 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rojas-Benítez, Diego
L. Allende, Miguel
Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
title Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
title_full Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
title_fullStr Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
title_full_unstemmed Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
title_short Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
title_sort elongator subunit 3 (elp3) is required for zebrafish trunk development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036906/
https://www.ncbi.nlm.nih.gov/pubmed/32023806
http://dx.doi.org/10.3390/ijms21030925
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