Cargando…

Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury

Acute pancreatitis is an inflammatory disorder of the pancreas. Its presentation ranges from self-limiting disease to acute necrotizing pancreatitis (ANP) with multiorgan failure and a high mortality. Polyethylene glycols (PEGs) are non-immunogenic, non-toxic, and water-soluble chemicals composed of...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferrero-Andrés, Ana, Panisello-Roselló, Arnau, Serafín, Anna, Roselló-Catafau, Joan, Folch-Puy, Emma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036920/
https://www.ncbi.nlm.nih.gov/pubmed/32019239
http://dx.doi.org/10.3390/ijms21030917
_version_ 1783500306225561600
author Ferrero-Andrés, Ana
Panisello-Roselló, Arnau
Serafín, Anna
Roselló-Catafau, Joan
Folch-Puy, Emma
author_facet Ferrero-Andrés, Ana
Panisello-Roselló, Arnau
Serafín, Anna
Roselló-Catafau, Joan
Folch-Puy, Emma
author_sort Ferrero-Andrés, Ana
collection PubMed
description Acute pancreatitis is an inflammatory disorder of the pancreas. Its presentation ranges from self-limiting disease to acute necrotizing pancreatitis (ANP) with multiorgan failure and a high mortality. Polyethylene glycols (PEGs) are non-immunogenic, non-toxic, and water-soluble chemicals composed of repeating units of ethylene glycol. The present article explores the effect of PEG35 administration on reducing the severity of ANP and associated lung injury. ANP was induced by injection of 5% sodium taurocholate into the biliopancreatic duct. PEG35 was administered intravenously either prophylactically or therapeutically. Three hours after ANP induction, pancreas and lung tissue samples and blood were collected and ANP severity was assessed. To evaluate the inflammatory response, gene expression of pro-inflammatory cytokines and chemokine and the changes in the presence of myeloperoxidase and adhesion molecule levels were determined in both the pancreas and the lung. To evaluate cell death, lactate dehydrogenase (LDH) activity and apoptotic cleaved caspase-3 localization were determined in plasma and in both the pancreatic and lung tissue respectively. ANP-associated local and systemic inflammatory processes were reduced when PEG35 was administered prophylactically. PEG35 pre-treatment also protected against acute pancreatitis-associated cell death. Notably, the therapeutic administration of PEG35 significantly decreased associated lung injury, even when the pancreatic lesion was equivalent to that in the untreated ANP-induced group. Our results support a protective role of PEG35 against the ANP-associated inflammatory process and identify PEG35 as a promising tool for the treatment of the potentially lethal complications of the disease.
format Online
Article
Text
id pubmed-7036920
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70369202020-03-11 Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury Ferrero-Andrés, Ana Panisello-Roselló, Arnau Serafín, Anna Roselló-Catafau, Joan Folch-Puy, Emma Int J Mol Sci Article Acute pancreatitis is an inflammatory disorder of the pancreas. Its presentation ranges from self-limiting disease to acute necrotizing pancreatitis (ANP) with multiorgan failure and a high mortality. Polyethylene glycols (PEGs) are non-immunogenic, non-toxic, and water-soluble chemicals composed of repeating units of ethylene glycol. The present article explores the effect of PEG35 administration on reducing the severity of ANP and associated lung injury. ANP was induced by injection of 5% sodium taurocholate into the biliopancreatic duct. PEG35 was administered intravenously either prophylactically or therapeutically. Three hours after ANP induction, pancreas and lung tissue samples and blood were collected and ANP severity was assessed. To evaluate the inflammatory response, gene expression of pro-inflammatory cytokines and chemokine and the changes in the presence of myeloperoxidase and adhesion molecule levels were determined in both the pancreas and the lung. To evaluate cell death, lactate dehydrogenase (LDH) activity and apoptotic cleaved caspase-3 localization were determined in plasma and in both the pancreatic and lung tissue respectively. ANP-associated local and systemic inflammatory processes were reduced when PEG35 was administered prophylactically. PEG35 pre-treatment also protected against acute pancreatitis-associated cell death. Notably, the therapeutic administration of PEG35 significantly decreased associated lung injury, even when the pancreatic lesion was equivalent to that in the untreated ANP-induced group. Our results support a protective role of PEG35 against the ANP-associated inflammatory process and identify PEG35 as a promising tool for the treatment of the potentially lethal complications of the disease. MDPI 2020-01-30 /pmc/articles/PMC7036920/ /pubmed/32019239 http://dx.doi.org/10.3390/ijms21030917 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferrero-Andrés, Ana
Panisello-Roselló, Arnau
Serafín, Anna
Roselló-Catafau, Joan
Folch-Puy, Emma
Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury
title Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury
title_full Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury
title_fullStr Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury
title_full_unstemmed Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury
title_short Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury
title_sort polyethylene glycol 35 (peg35) protects against inflammation in experimental acute necrotizing pancreatitis and associated lung injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036920/
https://www.ncbi.nlm.nih.gov/pubmed/32019239
http://dx.doi.org/10.3390/ijms21030917
work_keys_str_mv AT ferreroandresana polyethyleneglycol35peg35protectsagainstinflammationinexperimentalacutenecrotizingpancreatitisandassociatedlunginjury
AT paniselloroselloarnau polyethyleneglycol35peg35protectsagainstinflammationinexperimentalacutenecrotizingpancreatitisandassociatedlunginjury
AT serafinanna polyethyleneglycol35peg35protectsagainstinflammationinexperimentalacutenecrotizingpancreatitisandassociatedlunginjury
AT rosellocatafaujoan polyethyleneglycol35peg35protectsagainstinflammationinexperimentalacutenecrotizingpancreatitisandassociatedlunginjury
AT folchpuyemma polyethyleneglycol35peg35protectsagainstinflammationinexperimentalacutenecrotizingpancreatitisandassociatedlunginjury