Cargando…
RNA-Targeting CRISPR–Cas Systems and Their Applications
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR–Cas systems have been used as a backbone for the development of potent research t...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036953/ https://www.ncbi.nlm.nih.gov/pubmed/32046217 http://dx.doi.org/10.3390/ijms21031122 |
_version_ | 1783500314028015616 |
---|---|
author | Burmistrz, Michal Krakowski, Kamil Krawczyk-Balska, Agata |
author_facet | Burmistrz, Michal Krakowski, Kamil Krawczyk-Balska, Agata |
author_sort | Burmistrz, Michal |
collection | PubMed |
description | Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR–Cas systems have been used as a backbone for the development of potent research tools, with Cas9 being the most widespread. While most of the utilized systems are DNA-targeting, recently more and more attention is being gained by those that target RNA. Their ability to specifically recognize a given RNA sequence in an easily programmable way makes them ideal candidates for developing new research tools. In this review we summarize current knowledge on CRISPR–Cas systems which have been shown to target RNA molecules, that is type III (Csm/Cmr), type VI (Cas13), and type II (Cas9). We also present a list of available technologies based on these systems. |
format | Online Article Text |
id | pubmed-7036953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70369532020-03-11 RNA-Targeting CRISPR–Cas Systems and Their Applications Burmistrz, Michal Krakowski, Kamil Krawczyk-Balska, Agata Int J Mol Sci Review Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR–Cas systems have been used as a backbone for the development of potent research tools, with Cas9 being the most widespread. While most of the utilized systems are DNA-targeting, recently more and more attention is being gained by those that target RNA. Their ability to specifically recognize a given RNA sequence in an easily programmable way makes them ideal candidates for developing new research tools. In this review we summarize current knowledge on CRISPR–Cas systems which have been shown to target RNA molecules, that is type III (Csm/Cmr), type VI (Cas13), and type II (Cas9). We also present a list of available technologies based on these systems. MDPI 2020-02-07 /pmc/articles/PMC7036953/ /pubmed/32046217 http://dx.doi.org/10.3390/ijms21031122 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Burmistrz, Michal Krakowski, Kamil Krawczyk-Balska, Agata RNA-Targeting CRISPR–Cas Systems and Their Applications |
title | RNA-Targeting CRISPR–Cas Systems and Their Applications |
title_full | RNA-Targeting CRISPR–Cas Systems and Their Applications |
title_fullStr | RNA-Targeting CRISPR–Cas Systems and Their Applications |
title_full_unstemmed | RNA-Targeting CRISPR–Cas Systems and Their Applications |
title_short | RNA-Targeting CRISPR–Cas Systems and Their Applications |
title_sort | rna-targeting crispr–cas systems and their applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036953/ https://www.ncbi.nlm.nih.gov/pubmed/32046217 http://dx.doi.org/10.3390/ijms21031122 |
work_keys_str_mv | AT burmistrzmichal rnatargetingcrisprcassystemsandtheirapplications AT krakowskikamil rnatargetingcrisprcassystemsandtheirapplications AT krawczykbalskaagata rnatargetingcrisprcassystemsandtheirapplications |