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Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis
Pathogenic yeasts Candida albicans and Candida parapsilosis possess a ß-type carbonic anhydrase Nce103p, which is involved in CO(2) hydration and signaling. C. albicans lacking Nce103p cannot survive in low CO(2) concentrations, e.g., in atmospheric growth conditions. Candida carbonic anhydrases are...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036955/ https://www.ncbi.nlm.nih.gov/pubmed/32013007 http://dx.doi.org/10.3390/ijms21030850 |
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author | Dostál, Jiří Blaha, Jan Hadravová, Romana Hubálek, Martin Heidingsfeld, Olga Pichová, Iva |
author_facet | Dostál, Jiří Blaha, Jan Hadravová, Romana Hubálek, Martin Heidingsfeld, Olga Pichová, Iva |
author_sort | Dostál, Jiří |
collection | PubMed |
description | Pathogenic yeasts Candida albicans and Candida parapsilosis possess a ß-type carbonic anhydrase Nce103p, which is involved in CO(2) hydration and signaling. C. albicans lacking Nce103p cannot survive in low CO(2) concentrations, e.g., in atmospheric growth conditions. Candida carbonic anhydrases are orthologous to the Saccharomyces cerevisiae enzyme, which had originally been detected as a substrate of a non-classical export pathway. However, experimental evidence on localization of C. albicans and C. parapsilosis carbonic anhydrases has not been reported to date. Immunogold labeling and electron microscopy used in the present study showed that carbonic anhydrases are localized in the cell wall and plasmatic membrane of both Candida species. This localization was confirmed by Western blot and mass spectrometry analyses of isolated cell wall and plasma membrane fractions. Further analysis of C. albicans and C. parapsilosis subcellular fractions revealed presence of carbonic anhydrases also in the cytosolic and mitochondrial fractions of Candida cells cultivated in shaken liquid cultures, under the atmospheric conditions. |
format | Online Article Text |
id | pubmed-7036955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70369552020-03-11 Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis Dostál, Jiří Blaha, Jan Hadravová, Romana Hubálek, Martin Heidingsfeld, Olga Pichová, Iva Int J Mol Sci Article Pathogenic yeasts Candida albicans and Candida parapsilosis possess a ß-type carbonic anhydrase Nce103p, which is involved in CO(2) hydration and signaling. C. albicans lacking Nce103p cannot survive in low CO(2) concentrations, e.g., in atmospheric growth conditions. Candida carbonic anhydrases are orthologous to the Saccharomyces cerevisiae enzyme, which had originally been detected as a substrate of a non-classical export pathway. However, experimental evidence on localization of C. albicans and C. parapsilosis carbonic anhydrases has not been reported to date. Immunogold labeling and electron microscopy used in the present study showed that carbonic anhydrases are localized in the cell wall and plasmatic membrane of both Candida species. This localization was confirmed by Western blot and mass spectrometry analyses of isolated cell wall and plasma membrane fractions. Further analysis of C. albicans and C. parapsilosis subcellular fractions revealed presence of carbonic anhydrases also in the cytosolic and mitochondrial fractions of Candida cells cultivated in shaken liquid cultures, under the atmospheric conditions. MDPI 2020-01-28 /pmc/articles/PMC7036955/ /pubmed/32013007 http://dx.doi.org/10.3390/ijms21030850 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dostál, Jiří Blaha, Jan Hadravová, Romana Hubálek, Martin Heidingsfeld, Olga Pichová, Iva Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis |
title | Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis |
title_full | Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis |
title_fullStr | Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis |
title_full_unstemmed | Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis |
title_short | Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis |
title_sort | cellular localization of carbonic anhydrase nce103p in candida albicans and candida parapsilosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036955/ https://www.ncbi.nlm.nih.gov/pubmed/32013007 http://dx.doi.org/10.3390/ijms21030850 |
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