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Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan

Human bocavirus (HBoV) is a causative agent of respiratory and gastrointestinal diseases worldwide. Four HBoV species (HBoV1-4) have been identified so far. Although a previous report has documented the HBoV association with acute gastroenteritis (AGE) in Taiwan, their epidemiology, genetic diversit...

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Autores principales: Lin, Marco Yung-Cheng, Chan, Hsiu-Chuan, Chi, Hsin, Chiu, Shu-Chun, Nora-Krukle, Zaiga, Rasa-Dzelzkaleja, Santa, Vilmane, Anda, Murovska, Modra, Lin, Jih-Hui, Liu, Hsin-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037009/
https://www.ncbi.nlm.nih.gov/pubmed/32046345
http://dx.doi.org/10.3390/ijerph17031086
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author Lin, Marco Yung-Cheng
Chan, Hsiu-Chuan
Chi, Hsin
Chiu, Shu-Chun
Nora-Krukle, Zaiga
Rasa-Dzelzkaleja, Santa
Vilmane, Anda
Murovska, Modra
Lin, Jih-Hui
Liu, Hsin-Fu
author_facet Lin, Marco Yung-Cheng
Chan, Hsiu-Chuan
Chi, Hsin
Chiu, Shu-Chun
Nora-Krukle, Zaiga
Rasa-Dzelzkaleja, Santa
Vilmane, Anda
Murovska, Modra
Lin, Jih-Hui
Liu, Hsin-Fu
author_sort Lin, Marco Yung-Cheng
collection PubMed
description Human bocavirus (HBoV) is a causative agent of respiratory and gastrointestinal diseases worldwide. Four HBoV species (HBoV1-4) have been identified so far. Although a previous report has documented the HBoV association with acute gastroenteritis (AGE) in Taiwan, their epidemiology, genetic diversity, and phylogenetic relationships remain unclear. In this study, we focused on an investigation of these unsolved issues, which will help to reveal molecular epidemiology and phylogeny of the circulating HBoV2 in Taiwan. A total of 176 stool samples were collected from children with AGE for this study. PCR amplification and sequencing on the VP1 gene region were used to identify species. Phylogenetic analysis was conducted by maximum-likelihood and neighbor-joining methods. Selection pressure was also estimated to obtain HBoV evolutionary information. Our results showed the prevalence of HBoV in AGE children was 8.5%, of which HBoV1 was the predominant species (6.3%), followed by HBoV2 (2.3%). Phylogenetic analysis showed those Taiwanese HBoV2 strains have significant genetic variability and can be divided into two clusters. One belongs to HBoV2 genotype A and the other forms an independent unclassified cluster. The nucleotide distance between that independent cluster and the known HBoV2 genotypes was more than 5%, suggesting a new HBoV2 genotype. No positive selection site was found and the virus was under purifying selection. This is the first report to reveal HBoV2 genetic diversity and phylogenetic relationships among AGE children in Taiwan. We find that HBoV2 may have been introduced into the country by multiple origins, and a potential new HBoV2 genotype is proposed.
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spelling pubmed-70370092020-03-11 Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan Lin, Marco Yung-Cheng Chan, Hsiu-Chuan Chi, Hsin Chiu, Shu-Chun Nora-Krukle, Zaiga Rasa-Dzelzkaleja, Santa Vilmane, Anda Murovska, Modra Lin, Jih-Hui Liu, Hsin-Fu Int J Environ Res Public Health Article Human bocavirus (HBoV) is a causative agent of respiratory and gastrointestinal diseases worldwide. Four HBoV species (HBoV1-4) have been identified so far. Although a previous report has documented the HBoV association with acute gastroenteritis (AGE) in Taiwan, their epidemiology, genetic diversity, and phylogenetic relationships remain unclear. In this study, we focused on an investigation of these unsolved issues, which will help to reveal molecular epidemiology and phylogeny of the circulating HBoV2 in Taiwan. A total of 176 stool samples were collected from children with AGE for this study. PCR amplification and sequencing on the VP1 gene region were used to identify species. Phylogenetic analysis was conducted by maximum-likelihood and neighbor-joining methods. Selection pressure was also estimated to obtain HBoV evolutionary information. Our results showed the prevalence of HBoV in AGE children was 8.5%, of which HBoV1 was the predominant species (6.3%), followed by HBoV2 (2.3%). Phylogenetic analysis showed those Taiwanese HBoV2 strains have significant genetic variability and can be divided into two clusters. One belongs to HBoV2 genotype A and the other forms an independent unclassified cluster. The nucleotide distance between that independent cluster and the known HBoV2 genotypes was more than 5%, suggesting a new HBoV2 genotype. No positive selection site was found and the virus was under purifying selection. This is the first report to reveal HBoV2 genetic diversity and phylogenetic relationships among AGE children in Taiwan. We find that HBoV2 may have been introduced into the country by multiple origins, and a potential new HBoV2 genotype is proposed. MDPI 2020-02-08 2020-02 /pmc/articles/PMC7037009/ /pubmed/32046345 http://dx.doi.org/10.3390/ijerph17031086 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Marco Yung-Cheng
Chan, Hsiu-Chuan
Chi, Hsin
Chiu, Shu-Chun
Nora-Krukle, Zaiga
Rasa-Dzelzkaleja, Santa
Vilmane, Anda
Murovska, Modra
Lin, Jih-Hui
Liu, Hsin-Fu
Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan
title Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan
title_full Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan
title_fullStr Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan
title_full_unstemmed Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan
title_short Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan
title_sort genetic diversity and phylogenetic analysis of human bocavirus 2 in pediatric patients with acute gastroenteritis in taiwan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037009/
https://www.ncbi.nlm.nih.gov/pubmed/32046345
http://dx.doi.org/10.3390/ijerph17031086
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