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Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis

Placental inflammation and dysfunction during pregnancy are associated with short- and long-term adverse outcomes for the offspring. However, the mechanisms of vascular protection at the feto-placental interface are still poorly investigated. The high-density lipoprotein (HDL) associated sphingosine...

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Detalles Bibliográficos
Autores principales: Del Gaudio, Ilaria, Hendrix, Sebastian, Christoffersen, Christina, Wadsack, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037016/
https://www.ncbi.nlm.nih.gov/pubmed/31991780
http://dx.doi.org/10.3390/ijms21030789
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author Del Gaudio, Ilaria
Hendrix, Sebastian
Christoffersen, Christina
Wadsack, Christian
author_facet Del Gaudio, Ilaria
Hendrix, Sebastian
Christoffersen, Christina
Wadsack, Christian
author_sort Del Gaudio, Ilaria
collection PubMed
description Placental inflammation and dysfunction during pregnancy are associated with short- and long-term adverse outcomes for the offspring. However, the mechanisms of vascular protection at the feto-placental interface are still poorly investigated. The high-density lipoprotein (HDL) associated sphingosine-1-phosphate (S1P) has been described as a powerful anti-inflammatory complex. This study aimed to elucidate the role of cord blood-derived HDL (nHDL) in feto-placental endothelial dysfunction. Here, we report that the exposure of primary fetal placental arterial endothelial cell (fPAEC) to healthy nHDL-S1P attenuated the ability of TNFα to activate NF-κB signaling and increase the expression of pro-inflammatory markers. Moreover, the angiotensin II (AngII)-induced reactive oxygen species (ROS) production was blunted in the presence of nHDL, whereas it was preserved when the cells were preincubated with S1P receptor antagonists, suggesting that S1P accounts for the vascular protective function of nHDL at the feto-placental unit. These results highlight the importance of HDL and S1P metabolism and signaling in pregnancy pathophysiology.
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spelling pubmed-70370162020-03-11 Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis Del Gaudio, Ilaria Hendrix, Sebastian Christoffersen, Christina Wadsack, Christian Int J Mol Sci Communication Placental inflammation and dysfunction during pregnancy are associated with short- and long-term adverse outcomes for the offspring. However, the mechanisms of vascular protection at the feto-placental interface are still poorly investigated. The high-density lipoprotein (HDL) associated sphingosine-1-phosphate (S1P) has been described as a powerful anti-inflammatory complex. This study aimed to elucidate the role of cord blood-derived HDL (nHDL) in feto-placental endothelial dysfunction. Here, we report that the exposure of primary fetal placental arterial endothelial cell (fPAEC) to healthy nHDL-S1P attenuated the ability of TNFα to activate NF-κB signaling and increase the expression of pro-inflammatory markers. Moreover, the angiotensin II (AngII)-induced reactive oxygen species (ROS) production was blunted in the presence of nHDL, whereas it was preserved when the cells were preincubated with S1P receptor antagonists, suggesting that S1P accounts for the vascular protective function of nHDL at the feto-placental unit. These results highlight the importance of HDL and S1P metabolism and signaling in pregnancy pathophysiology. MDPI 2020-01-25 /pmc/articles/PMC7037016/ /pubmed/31991780 http://dx.doi.org/10.3390/ijms21030789 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Del Gaudio, Ilaria
Hendrix, Sebastian
Christoffersen, Christina
Wadsack, Christian
Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis
title Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis
title_full Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis
title_fullStr Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis
title_full_unstemmed Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis
title_short Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis
title_sort neonatal hdl counteracts placental vascular inflammation via s1p–s1pr1 axis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037016/
https://www.ncbi.nlm.nih.gov/pubmed/31991780
http://dx.doi.org/10.3390/ijms21030789
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