Cargando…
Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway
Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, pl...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037017/ https://www.ncbi.nlm.nih.gov/pubmed/31991762 http://dx.doi.org/10.3390/ijms21030784 |
_version_ | 1783500329029992448 |
---|---|
author | Ki, Ga-Eun Kim, Yu-Mi Lim, Han-Moi Lee, Eun-Cheol Choi, Yun-Kyong Seo, Young-Kwon |
author_facet | Ki, Ga-Eun Kim, Yu-Mi Lim, Han-Moi Lee, Eun-Cheol Choi, Yun-Kyong Seo, Young-Kwon |
author_sort | Ki, Ga-Eun |
collection | PubMed |
description | Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, play an essential role in controlling hair growth and in conditions like androgenic alopecia. This study aimed to evaluate the intensity-dependent effect of extremely low-frequency electromagnetic fields (ELF-EMFs) on the expression of anagen-related molecules in hDPCs in vitro. We examined the effect of ELF-EMF on hDPCs to determine whether activation of the GSK-3β/ERK/Akt signaling pathway improved hDPC activation and proliferation; hDPCs were exposed to ELF-EMFs at a frequency of 70 Hz and at intensities ranging from 5 to 100 G, over four days. Various PEMF intensities significantly increased the expression of anagen-related molecules, including collagen IV, laminin, ALP, and versican. In particular, an intensity of 10 G is most potent for promoting the proliferation of hDPC and expression of anagen-related molecules. Moreover, 10 G ELF-EMF significantly increased β-catenin and Wnt3α expression and GSK-3β/ERK/Akt phosphorylation. Our results confirmed that ELF-EMFs enhance hDPC activation and proliferation via the GSK-3β/ERK/Akt signaling pathway, suggesting a potential treatment strategy for alopecia. |
format | Online Article Text |
id | pubmed-7037017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70370172020-03-11 Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway Ki, Ga-Eun Kim, Yu-Mi Lim, Han-Moi Lee, Eun-Cheol Choi, Yun-Kyong Seo, Young-Kwon Int J Mol Sci Article Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, play an essential role in controlling hair growth and in conditions like androgenic alopecia. This study aimed to evaluate the intensity-dependent effect of extremely low-frequency electromagnetic fields (ELF-EMFs) on the expression of anagen-related molecules in hDPCs in vitro. We examined the effect of ELF-EMF on hDPCs to determine whether activation of the GSK-3β/ERK/Akt signaling pathway improved hDPC activation and proliferation; hDPCs were exposed to ELF-EMFs at a frequency of 70 Hz and at intensities ranging from 5 to 100 G, over four days. Various PEMF intensities significantly increased the expression of anagen-related molecules, including collagen IV, laminin, ALP, and versican. In particular, an intensity of 10 G is most potent for promoting the proliferation of hDPC and expression of anagen-related molecules. Moreover, 10 G ELF-EMF significantly increased β-catenin and Wnt3α expression and GSK-3β/ERK/Akt phosphorylation. Our results confirmed that ELF-EMFs enhance hDPC activation and proliferation via the GSK-3β/ERK/Akt signaling pathway, suggesting a potential treatment strategy for alopecia. MDPI 2020-01-25 /pmc/articles/PMC7037017/ /pubmed/31991762 http://dx.doi.org/10.3390/ijms21030784 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ki, Ga-Eun Kim, Yu-Mi Lim, Han-Moi Lee, Eun-Cheol Choi, Yun-Kyong Seo, Young-Kwon Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway |
title | Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway |
title_full | Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway |
title_fullStr | Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway |
title_full_unstemmed | Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway |
title_short | Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway |
title_sort | extremely low-frequency electromagnetic fields increase the expression of anagen-related molecules in human dermal papilla cells via gsk-3β/erk/akt signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037017/ https://www.ncbi.nlm.nih.gov/pubmed/31991762 http://dx.doi.org/10.3390/ijms21030784 |
work_keys_str_mv | AT kigaeun extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway AT kimyumi extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway AT limhanmoi extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway AT leeeuncheol extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway AT choiyunkyong extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway AT seoyoungkwon extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway |