Cargando…

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway

Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, pl...

Descripción completa

Detalles Bibliográficos
Autores principales: Ki, Ga-Eun, Kim, Yu-Mi, Lim, Han-Moi, Lee, Eun-Cheol, Choi, Yun-Kyong, Seo, Young-Kwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037017/
https://www.ncbi.nlm.nih.gov/pubmed/31991762
http://dx.doi.org/10.3390/ijms21030784
_version_ 1783500329029992448
author Ki, Ga-Eun
Kim, Yu-Mi
Lim, Han-Moi
Lee, Eun-Cheol
Choi, Yun-Kyong
Seo, Young-Kwon
author_facet Ki, Ga-Eun
Kim, Yu-Mi
Lim, Han-Moi
Lee, Eun-Cheol
Choi, Yun-Kyong
Seo, Young-Kwon
author_sort Ki, Ga-Eun
collection PubMed
description Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, play an essential role in controlling hair growth and in conditions like androgenic alopecia. This study aimed to evaluate the intensity-dependent effect of extremely low-frequency electromagnetic fields (ELF-EMFs) on the expression of anagen-related molecules in hDPCs in vitro. We examined the effect of ELF-EMF on hDPCs to determine whether activation of the GSK-3β/ERK/Akt signaling pathway improved hDPC activation and proliferation; hDPCs were exposed to ELF-EMFs at a frequency of 70 Hz and at intensities ranging from 5 to 100 G, over four days. Various PEMF intensities significantly increased the expression of anagen-related molecules, including collagen IV, laminin, ALP, and versican. In particular, an intensity of 10 G is most potent for promoting the proliferation of hDPC and expression of anagen-related molecules. Moreover, 10 G ELF-EMF significantly increased β-catenin and Wnt3α expression and GSK-3β/ERK/Akt phosphorylation. Our results confirmed that ELF-EMFs enhance hDPC activation and proliferation via the GSK-3β/ERK/Akt signaling pathway, suggesting a potential treatment strategy for alopecia.
format Online
Article
Text
id pubmed-7037017
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70370172020-03-11 Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway Ki, Ga-Eun Kim, Yu-Mi Lim, Han-Moi Lee, Eun-Cheol Choi, Yun-Kyong Seo, Young-Kwon Int J Mol Sci Article Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, play an essential role in controlling hair growth and in conditions like androgenic alopecia. This study aimed to evaluate the intensity-dependent effect of extremely low-frequency electromagnetic fields (ELF-EMFs) on the expression of anagen-related molecules in hDPCs in vitro. We examined the effect of ELF-EMF on hDPCs to determine whether activation of the GSK-3β/ERK/Akt signaling pathway improved hDPC activation and proliferation; hDPCs were exposed to ELF-EMFs at a frequency of 70 Hz and at intensities ranging from 5 to 100 G, over four days. Various PEMF intensities significantly increased the expression of anagen-related molecules, including collagen IV, laminin, ALP, and versican. In particular, an intensity of 10 G is most potent for promoting the proliferation of hDPC and expression of anagen-related molecules. Moreover, 10 G ELF-EMF significantly increased β-catenin and Wnt3α expression and GSK-3β/ERK/Akt phosphorylation. Our results confirmed that ELF-EMFs enhance hDPC activation and proliferation via the GSK-3β/ERK/Akt signaling pathway, suggesting a potential treatment strategy for alopecia. MDPI 2020-01-25 /pmc/articles/PMC7037017/ /pubmed/31991762 http://dx.doi.org/10.3390/ijms21030784 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ki, Ga-Eun
Kim, Yu-Mi
Lim, Han-Moi
Lee, Eun-Cheol
Choi, Yun-Kyong
Seo, Young-Kwon
Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway
title Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway
title_full Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway
title_fullStr Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway
title_full_unstemmed Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway
title_short Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway
title_sort extremely low-frequency electromagnetic fields increase the expression of anagen-related molecules in human dermal papilla cells via gsk-3β/erk/akt signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037017/
https://www.ncbi.nlm.nih.gov/pubmed/31991762
http://dx.doi.org/10.3390/ijms21030784
work_keys_str_mv AT kigaeun extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway
AT kimyumi extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway
AT limhanmoi extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway
AT leeeuncheol extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway
AT choiyunkyong extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway
AT seoyoungkwon extremelylowfrequencyelectromagneticfieldsincreasetheexpressionofanagenrelatedmoleculesinhumandermalpapillacellsviagsk3berkaktsignalingpathway