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Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery

Liposomes are extensively used in drug delivery, while alginates are widely used in tissue engineering. However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery. Herein, we de...

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Autores principales: Ding, Liang, Cui, Xinxia, Jiang, Rui, Zhou, Keya, Wen, Yalei, Wang, Chenfeng, Yue, Zhilian, Shen, Shigang, Pan, Xuefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037032/
https://www.ncbi.nlm.nih.gov/pubmed/32041216
http://dx.doi.org/10.3390/molecules25030694
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author Ding, Liang
Cui, Xinxia
Jiang, Rui
Zhou, Keya
Wen, Yalei
Wang, Chenfeng
Yue, Zhilian
Shen, Shigang
Pan, Xuefeng
author_facet Ding, Liang
Cui, Xinxia
Jiang, Rui
Zhou, Keya
Wen, Yalei
Wang, Chenfeng
Yue, Zhilian
Shen, Shigang
Pan, Xuefeng
author_sort Ding, Liang
collection PubMed
description Liposomes are extensively used in drug delivery, while alginates are widely used in tissue engineering. However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery. Herein, we developed a type of thermo-responsible liposome–alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate. The temperature-sensitivity of the liposomes was actualized by using phospholipids comprising dipalmitoylphosphatidylcholine (DPPC) and PS and the liposomes were prepared by a thin-film dispersion method. The TSPMAH was then successfully prepared by bridge-linking the microcapsules onto the alginate hydrogel via PS-Ca(2+)-Carboxyl-alginate interaction. Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively. Their rheological property was also characterized by using a rheometer. Cytotoxicity evaluations of the TSPMAH showed that the composite hydrogel was biocompatible, safe, and non-toxic. Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine–siRNA complex-carrying TSPMAH drug carriers. Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system.
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spelling pubmed-70370322020-03-11 Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery Ding, Liang Cui, Xinxia Jiang, Rui Zhou, Keya Wen, Yalei Wang, Chenfeng Yue, Zhilian Shen, Shigang Pan, Xuefeng Molecules Article Liposomes are extensively used in drug delivery, while alginates are widely used in tissue engineering. However, liposomes are usually thermally unstable and drug-leaking when in liquids, while the drug carriers made of alginates show low loading capacities when used for drug delivery. Herein, we developed a type of thermo-responsible liposome–alginate composite hydrogel (TSPMAH) by grafting thermo-responsive liposomes onto alginates by using Ca2+ mediated bonding between the phosphatidic serine (PS) in the liposome membrane and the alginate. The temperature-sensitivity of the liposomes was actualized by using phospholipids comprising dipalmitoylphosphatidylcholine (DPPC) and PS and the liposomes were prepared by a thin-film dispersion method. The TSPMAH was then successfully prepared by bridge-linking the microcapsules onto the alginate hydrogel via PS-Ca(2+)-Carboxyl-alginate interaction. Characterizations of the TSPMAH were carried out using scanning electron microscopy, transform infrared spectroscopy, and laser scanning confocal microscopy, respectively. Their rheological property was also characterized by using a rheometer. Cytotoxicity evaluations of the TSPMAH showed that the composite hydrogel was biocompatible, safe, and non-toxic. Further, loading and thermos-inducible release of model drugs encapsulated by the TSPMAH as a drug carrier system was also studied by making protamine–siRNA complex-carrying TSPMAH drug carriers. Our results indicated that the TSPMAH described herein has great potentials to be further developed into an intelligent drug delivery system. MDPI 2020-02-06 /pmc/articles/PMC7037032/ /pubmed/32041216 http://dx.doi.org/10.3390/molecules25030694 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Liang
Cui, Xinxia
Jiang, Rui
Zhou, Keya
Wen, Yalei
Wang, Chenfeng
Yue, Zhilian
Shen, Shigang
Pan, Xuefeng
Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery
title Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery
title_full Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery
title_fullStr Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery
title_full_unstemmed Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery
title_short Design, Synthesis and Characterization of a Novel Type of Thermo-Responsible Phospholipid Microcapsule–Alginate Composite Hydrogel for Drug Delivery
title_sort design, synthesis and characterization of a novel type of thermo-responsible phospholipid microcapsule–alginate composite hydrogel for drug delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037032/
https://www.ncbi.nlm.nih.gov/pubmed/32041216
http://dx.doi.org/10.3390/molecules25030694
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