Cargando…

Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells

The zinc finger transcription factor, Bcl11b, is expressed in T cells and group 2 innate lymphoid cells (ILC2s) among hematopoietic cells. In early T-lineage cells, Bcl11b directly binds and represses the gene encoding the E protein antagonist, Id2, preventing pro-T cells from adopting innate-like f...

Descripción completa

Detalles Bibliográficos
Autores principales: Hosokawa, Hiroyuki, Romero-Wolf, Maile, Yang, Qi, Motomura, Yasutaka, Levanon, Ditsa, Groner, Yoram, Moro, Kazuyo, Tanaka, Tomoaki, Rothenberg, Ellen V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037248/
https://www.ncbi.nlm.nih.gov/pubmed/31653691
http://dx.doi.org/10.1084/jem.20190972
_version_ 1783500382817746944
author Hosokawa, Hiroyuki
Romero-Wolf, Maile
Yang, Qi
Motomura, Yasutaka
Levanon, Ditsa
Groner, Yoram
Moro, Kazuyo
Tanaka, Tomoaki
Rothenberg, Ellen V.
author_facet Hosokawa, Hiroyuki
Romero-Wolf, Maile
Yang, Qi
Motomura, Yasutaka
Levanon, Ditsa
Groner, Yoram
Moro, Kazuyo
Tanaka, Tomoaki
Rothenberg, Ellen V.
author_sort Hosokawa, Hiroyuki
collection PubMed
description The zinc finger transcription factor, Bcl11b, is expressed in T cells and group 2 innate lymphoid cells (ILC2s) among hematopoietic cells. In early T-lineage cells, Bcl11b directly binds and represses the gene encoding the E protein antagonist, Id2, preventing pro-T cells from adopting innate-like fates. In contrast, ILC2s co-express both Bcl11b and Id2. To address this contradiction, we have directly compared Bcl11b action mechanisms in pro-T cells and ILC2s. We found that Bcl11b binding to regions across the genome shows distinct cell type–specific motif preferences. Bcl11b occupies functionally different sites in lineage-specific patterns and controls totally different sets of target genes in these cell types. In addition, Bcl11b bears cell type–specific post-translational modifications and organizes different cell type–specific protein complexes. However, both cell types use the same distal enhancer region to control timing of Bcl11b activation. Therefore, although pro-T cells and ILC2s both need Bcl11b for optimal development and function, Bcl11b works substantially differently in these two cell types.
format Online
Article
Text
id pubmed-7037248
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-70372482020-07-06 Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells Hosokawa, Hiroyuki Romero-Wolf, Maile Yang, Qi Motomura, Yasutaka Levanon, Ditsa Groner, Yoram Moro, Kazuyo Tanaka, Tomoaki Rothenberg, Ellen V. J Exp Med Research Articles The zinc finger transcription factor, Bcl11b, is expressed in T cells and group 2 innate lymphoid cells (ILC2s) among hematopoietic cells. In early T-lineage cells, Bcl11b directly binds and represses the gene encoding the E protein antagonist, Id2, preventing pro-T cells from adopting innate-like fates. In contrast, ILC2s co-express both Bcl11b and Id2. To address this contradiction, we have directly compared Bcl11b action mechanisms in pro-T cells and ILC2s. We found that Bcl11b binding to regions across the genome shows distinct cell type–specific motif preferences. Bcl11b occupies functionally different sites in lineage-specific patterns and controls totally different sets of target genes in these cell types. In addition, Bcl11b bears cell type–specific post-translational modifications and organizes different cell type–specific protein complexes. However, both cell types use the same distal enhancer region to control timing of Bcl11b activation. Therefore, although pro-T cells and ILC2s both need Bcl11b for optimal development and function, Bcl11b works substantially differently in these two cell types. Rockefeller University Press 2019-10-25 /pmc/articles/PMC7037248/ /pubmed/31653691 http://dx.doi.org/10.1084/jem.20190972 Text en © 2019 Hosokawa et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Hosokawa, Hiroyuki
Romero-Wolf, Maile
Yang, Qi
Motomura, Yasutaka
Levanon, Ditsa
Groner, Yoram
Moro, Kazuyo
Tanaka, Tomoaki
Rothenberg, Ellen V.
Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells
title Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells
title_full Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells
title_fullStr Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells
title_full_unstemmed Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells
title_short Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells
title_sort cell type–specific actions of bcl11b in early t-lineage and group 2 innate lymphoid cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037248/
https://www.ncbi.nlm.nih.gov/pubmed/31653691
http://dx.doi.org/10.1084/jem.20190972
work_keys_str_mv AT hosokawahiroyuki celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT romerowolfmaile celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT yangqi celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT motomurayasutaka celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT levanonditsa celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT groneryoram celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT morokazuyo celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT tanakatomoaki celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells
AT rothenbergellenv celltypespecificactionsofbcl11binearlytlineageandgroup2innatelymphoidcells