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Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells
The zinc finger transcription factor, Bcl11b, is expressed in T cells and group 2 innate lymphoid cells (ILC2s) among hematopoietic cells. In early T-lineage cells, Bcl11b directly binds and represses the gene encoding the E protein antagonist, Id2, preventing pro-T cells from adopting innate-like f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037248/ https://www.ncbi.nlm.nih.gov/pubmed/31653691 http://dx.doi.org/10.1084/jem.20190972 |
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author | Hosokawa, Hiroyuki Romero-Wolf, Maile Yang, Qi Motomura, Yasutaka Levanon, Ditsa Groner, Yoram Moro, Kazuyo Tanaka, Tomoaki Rothenberg, Ellen V. |
author_facet | Hosokawa, Hiroyuki Romero-Wolf, Maile Yang, Qi Motomura, Yasutaka Levanon, Ditsa Groner, Yoram Moro, Kazuyo Tanaka, Tomoaki Rothenberg, Ellen V. |
author_sort | Hosokawa, Hiroyuki |
collection | PubMed |
description | The zinc finger transcription factor, Bcl11b, is expressed in T cells and group 2 innate lymphoid cells (ILC2s) among hematopoietic cells. In early T-lineage cells, Bcl11b directly binds and represses the gene encoding the E protein antagonist, Id2, preventing pro-T cells from adopting innate-like fates. In contrast, ILC2s co-express both Bcl11b and Id2. To address this contradiction, we have directly compared Bcl11b action mechanisms in pro-T cells and ILC2s. We found that Bcl11b binding to regions across the genome shows distinct cell type–specific motif preferences. Bcl11b occupies functionally different sites in lineage-specific patterns and controls totally different sets of target genes in these cell types. In addition, Bcl11b bears cell type–specific post-translational modifications and organizes different cell type–specific protein complexes. However, both cell types use the same distal enhancer region to control timing of Bcl11b activation. Therefore, although pro-T cells and ILC2s both need Bcl11b for optimal development and function, Bcl11b works substantially differently in these two cell types. |
format | Online Article Text |
id | pubmed-7037248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70372482020-07-06 Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells Hosokawa, Hiroyuki Romero-Wolf, Maile Yang, Qi Motomura, Yasutaka Levanon, Ditsa Groner, Yoram Moro, Kazuyo Tanaka, Tomoaki Rothenberg, Ellen V. J Exp Med Research Articles The zinc finger transcription factor, Bcl11b, is expressed in T cells and group 2 innate lymphoid cells (ILC2s) among hematopoietic cells. In early T-lineage cells, Bcl11b directly binds and represses the gene encoding the E protein antagonist, Id2, preventing pro-T cells from adopting innate-like fates. In contrast, ILC2s co-express both Bcl11b and Id2. To address this contradiction, we have directly compared Bcl11b action mechanisms in pro-T cells and ILC2s. We found that Bcl11b binding to regions across the genome shows distinct cell type–specific motif preferences. Bcl11b occupies functionally different sites in lineage-specific patterns and controls totally different sets of target genes in these cell types. In addition, Bcl11b bears cell type–specific post-translational modifications and organizes different cell type–specific protein complexes. However, both cell types use the same distal enhancer region to control timing of Bcl11b activation. Therefore, although pro-T cells and ILC2s both need Bcl11b for optimal development and function, Bcl11b works substantially differently in these two cell types. Rockefeller University Press 2019-10-25 /pmc/articles/PMC7037248/ /pubmed/31653691 http://dx.doi.org/10.1084/jem.20190972 Text en © 2019 Hosokawa et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Hosokawa, Hiroyuki Romero-Wolf, Maile Yang, Qi Motomura, Yasutaka Levanon, Ditsa Groner, Yoram Moro, Kazuyo Tanaka, Tomoaki Rothenberg, Ellen V. Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells |
title | Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells |
title_full | Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells |
title_fullStr | Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells |
title_full_unstemmed | Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells |
title_short | Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells |
title_sort | cell type–specific actions of bcl11b in early t-lineage and group 2 innate lymphoid cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037248/ https://www.ncbi.nlm.nih.gov/pubmed/31653691 http://dx.doi.org/10.1084/jem.20190972 |
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