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Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493

Root-knot nematode diseases cause severe yield and economic losses each year in global agricultural production. Virgibacillus dokdonensis MCCC 1A00493, a deep-sea bacterium, shows a significant nematicidal activity against Meloidogyne incognita in vitro. However, information about the active substan...

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Autores principales: Huang, Dian, Yu, Chen, Shao, Zongze, Cai, Minmin, Li, Guangyu, Zheng, Longyu, Yu, Ziniu, Zhang, Jibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037310/
https://www.ncbi.nlm.nih.gov/pubmed/32050419
http://dx.doi.org/10.3390/molecules25030744
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author Huang, Dian
Yu, Chen
Shao, Zongze
Cai, Minmin
Li, Guangyu
Zheng, Longyu
Yu, Ziniu
Zhang, Jibin
author_facet Huang, Dian
Yu, Chen
Shao, Zongze
Cai, Minmin
Li, Guangyu
Zheng, Longyu
Yu, Ziniu
Zhang, Jibin
author_sort Huang, Dian
collection PubMed
description Root-knot nematode diseases cause severe yield and economic losses each year in global agricultural production. Virgibacillus dokdonensis MCCC 1A00493, a deep-sea bacterium, shows a significant nematicidal activity against Meloidogyne incognita in vitro. However, information about the active substances of V. dokdonensis MCCC 1A00493 is limited. In this study, volatile organic compounds (VOCs) from V. dokdonensis MCCC 1A00493 were isolated and analyzed through solid-phase microextraction and gas chromatography–mass spectrometry. Four VOCs, namely, acetaldehyde, dimethyl disulfide, ethylbenzene, and 2-butanone, were identified, and their nematicidal activities were evaluated. The four VOCs had a variety of active modes on M. incognita juveniles. Acetaldehyde had direct contact killing, fumigation, and attraction activities; dimethyl disulfide had direct contact killing and attraction activities; ethylbenzene had an attraction activity; and 2-butanone had a repellent activity. Only acetaldehyde had a fumigant activity to inhibit egg hatching. Combining this fumigant activity against eggs and juveniles could be an effective strategy to control the different developmental stages of M. incognita. The combination of direct contact and attraction activities could also establish trapping and killing strategies against root-knot nematodes. Considering all nematicidal modes or strategies, we could use V. dokdonensis MCCC 1A00493 to set up an integrated strategy to control root-knot nematodes.
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spelling pubmed-70373102020-03-11 Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493 Huang, Dian Yu, Chen Shao, Zongze Cai, Minmin Li, Guangyu Zheng, Longyu Yu, Ziniu Zhang, Jibin Molecules Article Root-knot nematode diseases cause severe yield and economic losses each year in global agricultural production. Virgibacillus dokdonensis MCCC 1A00493, a deep-sea bacterium, shows a significant nematicidal activity against Meloidogyne incognita in vitro. However, information about the active substances of V. dokdonensis MCCC 1A00493 is limited. In this study, volatile organic compounds (VOCs) from V. dokdonensis MCCC 1A00493 were isolated and analyzed through solid-phase microextraction and gas chromatography–mass spectrometry. Four VOCs, namely, acetaldehyde, dimethyl disulfide, ethylbenzene, and 2-butanone, were identified, and their nematicidal activities were evaluated. The four VOCs had a variety of active modes on M. incognita juveniles. Acetaldehyde had direct contact killing, fumigation, and attraction activities; dimethyl disulfide had direct contact killing and attraction activities; ethylbenzene had an attraction activity; and 2-butanone had a repellent activity. Only acetaldehyde had a fumigant activity to inhibit egg hatching. Combining this fumigant activity against eggs and juveniles could be an effective strategy to control the different developmental stages of M. incognita. The combination of direct contact and attraction activities could also establish trapping and killing strategies against root-knot nematodes. Considering all nematicidal modes or strategies, we could use V. dokdonensis MCCC 1A00493 to set up an integrated strategy to control root-knot nematodes. MDPI 2020-02-09 /pmc/articles/PMC7037310/ /pubmed/32050419 http://dx.doi.org/10.3390/molecules25030744 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Dian
Yu, Chen
Shao, Zongze
Cai, Minmin
Li, Guangyu
Zheng, Longyu
Yu, Ziniu
Zhang, Jibin
Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493
title Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493
title_full Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493
title_fullStr Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493
title_full_unstemmed Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493
title_short Identification and Characterization of Nnematicidal Volatile Organic Compounds from Deep-Sea Virgibacillus dokdonensis MCCC 1A00493
title_sort identification and characterization of nnematicidal volatile organic compounds from deep-sea virgibacillus dokdonensis mccc 1a00493
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037310/
https://www.ncbi.nlm.nih.gov/pubmed/32050419
http://dx.doi.org/10.3390/molecules25030744
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