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Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables

Microfluidic flow chambers (MFCs) allow the study of platelet adhesion and thrombus formation under flow, which may be influenced by several variables. We developed a new MFC, with which we tested the effects of different variables on the results of platelet deposition and thrombus formation on a co...

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Autores principales: Scavone, Mariangela, Bozzi, Silvia, Mencarini, Tatiana, Podda, Gianmarco, Cattaneo, Marco, Redaelli, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037340/
https://www.ncbi.nlm.nih.gov/pubmed/31979370
http://dx.doi.org/10.3390/ijms21030750
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author Scavone, Mariangela
Bozzi, Silvia
Mencarini, Tatiana
Podda, Gianmarco
Cattaneo, Marco
Redaelli, Alberto
author_facet Scavone, Mariangela
Bozzi, Silvia
Mencarini, Tatiana
Podda, Gianmarco
Cattaneo, Marco
Redaelli, Alberto
author_sort Scavone, Mariangela
collection PubMed
description Microfluidic flow chambers (MFCs) allow the study of platelet adhesion and thrombus formation under flow, which may be influenced by several variables. We developed a new MFC, with which we tested the effects of different variables on the results of platelet deposition and thrombus formation on a collagen-coated surface. Methods: Whole blood was perfused in the MFC over collagen Type I for 4 min at different wall shear rates (WSR) and different concentrations of collagen-coating solutions, keeping blood samples at room temperature or 37 °C before starting the experiments. In addition, we tested the effects of the antiplatelet agent acetylsalicylic acid (ASA) (antagonist of cyclooxygenase-1, 100 µM) and cangrelor (antagonist of P2Y(12), 1 µM). Results: Platelet deposition on collagen (I) was not affected by the storage temperature of the blood before perfusion (room temperature vs. 37 °C); (II) was dependent on a shear rate in the range between 300/s and 1700/s; and (III) was influenced by the collagen concentration used to coat the microchannels up to a value of 10 µg/mL. ASA and cangrelor did not cause statistically significant inhibition of platelet accumulation, except for ASA at low collagen concentrations. Conclusions: Platelet deposition on collagen-coated surfaces is a shear-dependent process, not influenced by the collagen concentration beyond a value of 10 µg/mL. However, the inhibitory effect of antiplatelet drugs is better observed using low concentrations of collagen.
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spelling pubmed-70373402020-03-11 Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables Scavone, Mariangela Bozzi, Silvia Mencarini, Tatiana Podda, Gianmarco Cattaneo, Marco Redaelli, Alberto Int J Mol Sci Article Microfluidic flow chambers (MFCs) allow the study of platelet adhesion and thrombus formation under flow, which may be influenced by several variables. We developed a new MFC, with which we tested the effects of different variables on the results of platelet deposition and thrombus formation on a collagen-coated surface. Methods: Whole blood was perfused in the MFC over collagen Type I for 4 min at different wall shear rates (WSR) and different concentrations of collagen-coating solutions, keeping blood samples at room temperature or 37 °C before starting the experiments. In addition, we tested the effects of the antiplatelet agent acetylsalicylic acid (ASA) (antagonist of cyclooxygenase-1, 100 µM) and cangrelor (antagonist of P2Y(12), 1 µM). Results: Platelet deposition on collagen (I) was not affected by the storage temperature of the blood before perfusion (room temperature vs. 37 °C); (II) was dependent on a shear rate in the range between 300/s and 1700/s; and (III) was influenced by the collagen concentration used to coat the microchannels up to a value of 10 µg/mL. ASA and cangrelor did not cause statistically significant inhibition of platelet accumulation, except for ASA at low collagen concentrations. Conclusions: Platelet deposition on collagen-coated surfaces is a shear-dependent process, not influenced by the collagen concentration beyond a value of 10 µg/mL. However, the inhibitory effect of antiplatelet drugs is better observed using low concentrations of collagen. MDPI 2020-01-23 /pmc/articles/PMC7037340/ /pubmed/31979370 http://dx.doi.org/10.3390/ijms21030750 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scavone, Mariangela
Bozzi, Silvia
Mencarini, Tatiana
Podda, Gianmarco
Cattaneo, Marco
Redaelli, Alberto
Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables
title Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables
title_full Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables
title_fullStr Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables
title_full_unstemmed Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables
title_short Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables
title_sort platelet adhesion and thrombus formation in microchannels: the effect of assay-dependent variables
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037340/
https://www.ncbi.nlm.nih.gov/pubmed/31979370
http://dx.doi.org/10.3390/ijms21030750
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