Cargando…

Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia

Insect saliva induces significant antibody responses associated with the intensity of exposure to bites and the risk of disease in humans. Several salivary biomarkers have been characterized to determine exposure intensity to Old World Anopheles mosquito species. However, new tools are needed to qua...

Descripción completa

Detalles Bibliográficos
Autores principales: Londono-Renteria, Berlin, Drame, Papa M., Montiel, Jehidys, Vasquez, Ana M., Tobón-Castaño, Alberto, Taylor, Marissa, Vizcaino, Lucrecia, Lenhart, Audrey E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037407/
https://www.ncbi.nlm.nih.gov/pubmed/31973044
http://dx.doi.org/10.3390/ijms21030691
_version_ 1783500420853792768
author Londono-Renteria, Berlin
Drame, Papa M.
Montiel, Jehidys
Vasquez, Ana M.
Tobón-Castaño, Alberto
Taylor, Marissa
Vizcaino, Lucrecia
Lenhart, Audrey E.
author_facet Londono-Renteria, Berlin
Drame, Papa M.
Montiel, Jehidys
Vasquez, Ana M.
Tobón-Castaño, Alberto
Taylor, Marissa
Vizcaino, Lucrecia
Lenhart, Audrey E.
author_sort Londono-Renteria, Berlin
collection PubMed
description Insect saliva induces significant antibody responses associated with the intensity of exposure to bites and the risk of disease in humans. Several salivary biomarkers have been characterized to determine exposure intensity to Old World Anopheles mosquito species. However, new tools are needed to quantify the intensity of human exposure to Anopheles bites and understand the risk of malaria in low-transmission areas in the Americas. To address this need, we conducted proteomic and bioinformatic analyses of immunogenic candidate proteins present in the saliva of uninfected Anopheles albimanus from two separate colonies—one originating from Central America (STECLA strain) and one originating from South America (Cartagena strain). A ~65 kDa band was identified by IgG antibodies in serum samples from healthy volunteers living in a malaria endemic area in Colombia, and a total of five peptides were designed from the sequences of two immunogenic candidate proteins that were shared by both strains. ELISA-based testing of human IgG antibody levels against the peptides revealed that the transferrin-derived peptides, TRANS-P1, TRANS-P2 and a salivary peroxidase peptide (PEROX-P3) were able to distinguish between malaria-infected and uninfected groups. Interestingly, IgG antibody levels against PEROX-P3 were significantly lower in people that have never experienced malaria, suggesting that it may be a good marker for mosquito bite exposure in naïve populations such as travelers and deployed military personnel. In addition, the strength of the differences in the IgG levels against the peptides varied according to location, suggesting that the peptides may able to detect differences in intensities of bite exposure according to the mosquito population density. Thus, the An. albimanus salivary peptides TRANS-P1, TRANS-P2, and PEROX-P3 are promising biomarkers that could be exploited in a quantitative immunoassay for determination of human-vector contact and calculation of disease risk.
format Online
Article
Text
id pubmed-7037407
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70374072020-03-11 Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia Londono-Renteria, Berlin Drame, Papa M. Montiel, Jehidys Vasquez, Ana M. Tobón-Castaño, Alberto Taylor, Marissa Vizcaino, Lucrecia Lenhart, Audrey E. Int J Mol Sci Article Insect saliva induces significant antibody responses associated with the intensity of exposure to bites and the risk of disease in humans. Several salivary biomarkers have been characterized to determine exposure intensity to Old World Anopheles mosquito species. However, new tools are needed to quantify the intensity of human exposure to Anopheles bites and understand the risk of malaria in low-transmission areas in the Americas. To address this need, we conducted proteomic and bioinformatic analyses of immunogenic candidate proteins present in the saliva of uninfected Anopheles albimanus from two separate colonies—one originating from Central America (STECLA strain) and one originating from South America (Cartagena strain). A ~65 kDa band was identified by IgG antibodies in serum samples from healthy volunteers living in a malaria endemic area in Colombia, and a total of five peptides were designed from the sequences of two immunogenic candidate proteins that were shared by both strains. ELISA-based testing of human IgG antibody levels against the peptides revealed that the transferrin-derived peptides, TRANS-P1, TRANS-P2 and a salivary peroxidase peptide (PEROX-P3) were able to distinguish between malaria-infected and uninfected groups. Interestingly, IgG antibody levels against PEROX-P3 were significantly lower in people that have never experienced malaria, suggesting that it may be a good marker for mosquito bite exposure in naïve populations such as travelers and deployed military personnel. In addition, the strength of the differences in the IgG levels against the peptides varied according to location, suggesting that the peptides may able to detect differences in intensities of bite exposure according to the mosquito population density. Thus, the An. albimanus salivary peptides TRANS-P1, TRANS-P2, and PEROX-P3 are promising biomarkers that could be exploited in a quantitative immunoassay for determination of human-vector contact and calculation of disease risk. MDPI 2020-01-21 /pmc/articles/PMC7037407/ /pubmed/31973044 http://dx.doi.org/10.3390/ijms21030691 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Londono-Renteria, Berlin
Drame, Papa M.
Montiel, Jehidys
Vasquez, Ana M.
Tobón-Castaño, Alberto
Taylor, Marissa
Vizcaino, Lucrecia
Lenhart, Audrey E.
Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia
title Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia
title_full Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia
title_fullStr Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia
title_full_unstemmed Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia
title_short Identification and Pilot Evaluation of Salivary Peptides from Anopheles albimanus as Biomarkers for Bite Exposure and Malaria Infection in Colombia
title_sort identification and pilot evaluation of salivary peptides from anopheles albimanus as biomarkers for bite exposure and malaria infection in colombia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037407/
https://www.ncbi.nlm.nih.gov/pubmed/31973044
http://dx.doi.org/10.3390/ijms21030691
work_keys_str_mv AT londonorenteriaberlin identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia
AT dramepapam identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia
AT montieljehidys identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia
AT vasquezanam identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia
AT toboncastanoalberto identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia
AT taylormarissa identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia
AT vizcainolucrecia identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia
AT lenhartaudreye identificationandpilotevaluationofsalivarypeptidesfromanophelesalbimanusasbiomarkersforbiteexposureandmalariainfectionincolombia