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GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana

DELLA (GAI/RGA/RGL1/RGL2/RGL3) proteins are key negative regulators in GA (gibberellin) signaling and are involved in regulating plant growth as a response to environmental stresses. It has been shown that the DELLA protein PROCERA (PRO) in tomato promotes drought tolerance, but its molecular mechan...

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Autores principales: Wang, Zhijuan, Liu, Liu, Cheng, Chunhong, Ren, Ziyin, Xu, Shimin, Li, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037545/
https://www.ncbi.nlm.nih.gov/pubmed/32012796
http://dx.doi.org/10.3390/ijms21030819
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author Wang, Zhijuan
Liu, Liu
Cheng, Chunhong
Ren, Ziyin
Xu, Shimin
Li, Xia
author_facet Wang, Zhijuan
Liu, Liu
Cheng, Chunhong
Ren, Ziyin
Xu, Shimin
Li, Xia
author_sort Wang, Zhijuan
collection PubMed
description DELLA (GAI/RGA/RGL1/RGL2/RGL3) proteins are key negative regulators in GA (gibberellin) signaling and are involved in regulating plant growth as a response to environmental stresses. It has been shown that the DELLA protein PROCERA (PRO) in tomato promotes drought tolerance, but its molecular mechanism remains unknown. Here, we showed that the gai-1 (gibberellin insensitive 1) mutant (generated from the gai-1 (Ler) allele (with a 17 amino acid deletion within the DELLA domain of GAI) by backcrossing gai-1 (Ler) with Col-0 three times), the gain-of-function mutant of GAI (GA INSENSITIVE) in Arabidopsis, increases drought tolerance. The stomatal density of the gai-1 mutant was increased but its stomatal aperture was decreased under abscisic acid (ABA) treatment conditions, suggesting that the drought tolerance of the gai-1 mutant is a complex trait. We further tested the interactions between DELLA proteins and ABF2 (abscisic acid (ABA)-responsive element (ABRE)-binding transcription factors) and found that there was a strong interaction between DELLA proteins and ABF2. Our results provide new insight into DELLA proteins and their role in drought stress tolerance.
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spelling pubmed-70375452020-03-11 GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana Wang, Zhijuan Liu, Liu Cheng, Chunhong Ren, Ziyin Xu, Shimin Li, Xia Int J Mol Sci Communication DELLA (GAI/RGA/RGL1/RGL2/RGL3) proteins are key negative regulators in GA (gibberellin) signaling and are involved in regulating plant growth as a response to environmental stresses. It has been shown that the DELLA protein PROCERA (PRO) in tomato promotes drought tolerance, but its molecular mechanism remains unknown. Here, we showed that the gai-1 (gibberellin insensitive 1) mutant (generated from the gai-1 (Ler) allele (with a 17 amino acid deletion within the DELLA domain of GAI) by backcrossing gai-1 (Ler) with Col-0 three times), the gain-of-function mutant of GAI (GA INSENSITIVE) in Arabidopsis, increases drought tolerance. The stomatal density of the gai-1 mutant was increased but its stomatal aperture was decreased under abscisic acid (ABA) treatment conditions, suggesting that the drought tolerance of the gai-1 mutant is a complex trait. We further tested the interactions between DELLA proteins and ABF2 (abscisic acid (ABA)-responsive element (ABRE)-binding transcription factors) and found that there was a strong interaction between DELLA proteins and ABF2. Our results provide new insight into DELLA proteins and their role in drought stress tolerance. MDPI 2020-01-27 /pmc/articles/PMC7037545/ /pubmed/32012796 http://dx.doi.org/10.3390/ijms21030819 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Wang, Zhijuan
Liu, Liu
Cheng, Chunhong
Ren, Ziyin
Xu, Shimin
Li, Xia
GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana
title GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana
title_full GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana
title_fullStr GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana
title_full_unstemmed GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana
title_short GAI Functions in the Plant Response to Dehydration Stress in Arabidopsis thaliana
title_sort gai functions in the plant response to dehydration stress in arabidopsis thaliana
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037545/
https://www.ncbi.nlm.nih.gov/pubmed/32012796
http://dx.doi.org/10.3390/ijms21030819
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