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The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression
Megakaryopoiesis is the process during which megakaryoblasts differentiate to polyploid megakaryocytes that can subsequently shed thousands of platelets in the circulation. Megakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoske...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037754/ https://www.ncbi.nlm.nih.gov/pubmed/32024018 http://dx.doi.org/10.3390/ijms21030967 |
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author | Hansen, Marten Zeddies, Sabrina Meinders, Marjolein di Summa, Franca Rollmann, Ewa van Alphen, Floris P.J. Hoogendijk, Arjan J. Moore, Kat S. Halbach, Melanie Gutiérrez, Laura van den Biggelaar, Maartje Thijssen-Timmer, Daphne C. Auburger, Georg W.J. van den Akker, Emile von Lindern, Marieke |
author_facet | Hansen, Marten Zeddies, Sabrina Meinders, Marjolein di Summa, Franca Rollmann, Ewa van Alphen, Floris P.J. Hoogendijk, Arjan J. Moore, Kat S. Halbach, Melanie Gutiérrez, Laura van den Biggelaar, Maartje Thijssen-Timmer, Daphne C. Auburger, Georg W.J. van den Akker, Emile von Lindern, Marieke |
author_sort | Hansen, Marten |
collection | PubMed |
description | Megakaryopoiesis is the process during which megakaryoblasts differentiate to polyploid megakaryocytes that can subsequently shed thousands of platelets in the circulation. Megakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoskeletal proteins, membranes and platelet-specific granules, and for the subsequent shedding of thousands of platelets per cell. Gene expression profiling identified the RNA binding protein ATAXIN2 (ATXN2) as a putative novel regulator of megakaryopoiesis. ATXN2 expression is high in CD34(+)/CD41(+) megakaryoblasts and sharply decreases upon maturation to megakaryocytes. ATXN2 associates with DDX6 suggesting that it may mediate repression of mRNA translation during early megakaryopoiesis. Comparative transcriptome and proteome analysis on megakaryoid cells (MEG-01) with differential ATXN2 expression identified ATXN2 dependent gene expression of mRNA and protein involved in processes linked to hemostasis. Mice deficient for Atxn2 did not display differences in bleeding times, but the expression of key surface receptors on platelets, such as ITGB3 (carries the CD61 antigen) and CD31 (PECAM1), was deregulated and platelet aggregation upon specific triggers was reduced. |
format | Online Article Text |
id | pubmed-7037754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70377542020-03-10 The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression Hansen, Marten Zeddies, Sabrina Meinders, Marjolein di Summa, Franca Rollmann, Ewa van Alphen, Floris P.J. Hoogendijk, Arjan J. Moore, Kat S. Halbach, Melanie Gutiérrez, Laura van den Biggelaar, Maartje Thijssen-Timmer, Daphne C. Auburger, Georg W.J. van den Akker, Emile von Lindern, Marieke Int J Mol Sci Article Megakaryopoiesis is the process during which megakaryoblasts differentiate to polyploid megakaryocytes that can subsequently shed thousands of platelets in the circulation. Megakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoskeletal proteins, membranes and platelet-specific granules, and for the subsequent shedding of thousands of platelets per cell. Gene expression profiling identified the RNA binding protein ATAXIN2 (ATXN2) as a putative novel regulator of megakaryopoiesis. ATXN2 expression is high in CD34(+)/CD41(+) megakaryoblasts and sharply decreases upon maturation to megakaryocytes. ATXN2 associates with DDX6 suggesting that it may mediate repression of mRNA translation during early megakaryopoiesis. Comparative transcriptome and proteome analysis on megakaryoid cells (MEG-01) with differential ATXN2 expression identified ATXN2 dependent gene expression of mRNA and protein involved in processes linked to hemostasis. Mice deficient for Atxn2 did not display differences in bleeding times, but the expression of key surface receptors on platelets, such as ITGB3 (carries the CD61 antigen) and CD31 (PECAM1), was deregulated and platelet aggregation upon specific triggers was reduced. MDPI 2020-01-31 /pmc/articles/PMC7037754/ /pubmed/32024018 http://dx.doi.org/10.3390/ijms21030967 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hansen, Marten Zeddies, Sabrina Meinders, Marjolein di Summa, Franca Rollmann, Ewa van Alphen, Floris P.J. Hoogendijk, Arjan J. Moore, Kat S. Halbach, Melanie Gutiérrez, Laura van den Biggelaar, Maartje Thijssen-Timmer, Daphne C. Auburger, Georg W.J. van den Akker, Emile von Lindern, Marieke The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression |
title | The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression |
title_full | The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression |
title_fullStr | The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression |
title_full_unstemmed | The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression |
title_short | The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression |
title_sort | rna-binding protein atxn2 is expressed during megakaryopoiesis and may control timing of gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037754/ https://www.ncbi.nlm.nih.gov/pubmed/32024018 http://dx.doi.org/10.3390/ijms21030967 |
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