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Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo

The human specific bacterial pathogen Helicobacter pylori (H. pylori) is associated with severe gastric diseases, including gastric cancer. Recently, the increasing resistance makes the usage of antibiotics less effectively. Therefore, development of a new antimicrobial agent is required to control...

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Autores principales: Woo, Hyun Jun, Yang, Ji Yeong, Lee, Min Ho, Kim, Hyun Woo, Kwon, Hye Jin, Park, Min, Kim, Sung-kyu, Park, So-Young, Kim, Sa-Hyun, Kim, Jong-Bae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037973/
https://www.ncbi.nlm.nih.gov/pubmed/32028744
http://dx.doi.org/10.3390/ijms21031008
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author Woo, Hyun Jun
Yang, Ji Yeong
Lee, Min Ho
Kim, Hyun Woo
Kwon, Hye Jin
Park, Min
Kim, Sung-kyu
Park, So-Young
Kim, Sa-Hyun
Kim, Jong-Bae
author_facet Woo, Hyun Jun
Yang, Ji Yeong
Lee, Min Ho
Kim, Hyun Woo
Kwon, Hye Jin
Park, Min
Kim, Sung-kyu
Park, So-Young
Kim, Sa-Hyun
Kim, Jong-Bae
author_sort Woo, Hyun Jun
collection PubMed
description The human specific bacterial pathogen Helicobacter pylori (H. pylori) is associated with severe gastric diseases, including gastric cancer. Recently, the increasing resistance makes the usage of antibiotics less effectively. Therefore, development of a new antimicrobial agent is required to control H. pylori infection. In the current study, the inhibitory effect of β-caryophyllene on H. pylori growth, as well as the antibacterial therapeutic effect, has been demonstrated. β-caryophyllene inhibited H. pylori growth via the downregulation of dnaE, dnaN, holB, and gyrA and also downregulated virulence factors such as CagA, VacA, and SecA proteins. β-caryophyllene inhibited expression of several T4SS components, so that CagA translocation into H. pylori-infected AGS gastric cancer cells was decreased by β-caryophyllene treatment. β-caryophyllene also inhibited VacA entry through the downregulation of T5(a)SS. After β-caryophyllene administration on Mongolian gerbils, the immunohistochemistry (IHC) and Hematoxylin&Eosin stains showed therapeutic effects in the treated groups. Hematological data, which was consistent with histological data, support the therapeutic effect of β-caryophyllene administration. Such a positive effect of β-caryophyllene on H. pylori infection potently substantiates the natural compound as being capable of being used as a new antimicrobial agent or functional health food to help patients who are suffering from gastroduodenal diseases due to H. pylori infection.
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spelling pubmed-70379732020-03-10 Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo Woo, Hyun Jun Yang, Ji Yeong Lee, Min Ho Kim, Hyun Woo Kwon, Hye Jin Park, Min Kim, Sung-kyu Park, So-Young Kim, Sa-Hyun Kim, Jong-Bae Int J Mol Sci Article The human specific bacterial pathogen Helicobacter pylori (H. pylori) is associated with severe gastric diseases, including gastric cancer. Recently, the increasing resistance makes the usage of antibiotics less effectively. Therefore, development of a new antimicrobial agent is required to control H. pylori infection. In the current study, the inhibitory effect of β-caryophyllene on H. pylori growth, as well as the antibacterial therapeutic effect, has been demonstrated. β-caryophyllene inhibited H. pylori growth via the downregulation of dnaE, dnaN, holB, and gyrA and also downregulated virulence factors such as CagA, VacA, and SecA proteins. β-caryophyllene inhibited expression of several T4SS components, so that CagA translocation into H. pylori-infected AGS gastric cancer cells was decreased by β-caryophyllene treatment. β-caryophyllene also inhibited VacA entry through the downregulation of T5(a)SS. After β-caryophyllene administration on Mongolian gerbils, the immunohistochemistry (IHC) and Hematoxylin&Eosin stains showed therapeutic effects in the treated groups. Hematological data, which was consistent with histological data, support the therapeutic effect of β-caryophyllene administration. Such a positive effect of β-caryophyllene on H. pylori infection potently substantiates the natural compound as being capable of being used as a new antimicrobial agent or functional health food to help patients who are suffering from gastroduodenal diseases due to H. pylori infection. MDPI 2020-02-03 /pmc/articles/PMC7037973/ /pubmed/32028744 http://dx.doi.org/10.3390/ijms21031008 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Woo, Hyun Jun
Yang, Ji Yeong
Lee, Min Ho
Kim, Hyun Woo
Kwon, Hye Jin
Park, Min
Kim, Sung-kyu
Park, So-Young
Kim, Sa-Hyun
Kim, Jong-Bae
Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo
title Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo
title_full Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo
title_fullStr Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo
title_full_unstemmed Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo
title_short Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo
title_sort inhibitory effects of β-caryophyllene on helicobacter pylori infection in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037973/
https://www.ncbi.nlm.nih.gov/pubmed/32028744
http://dx.doi.org/10.3390/ijms21031008
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