Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens

The Amano lipase from Pseudomonas fluorescens (L-AK) was covalently immobilized on various carbon nanomaterials (functionalized single-walled carbon nanotubes and graphene oxide) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized L-AK on SwCNT(NH2)...

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Autores principales: Bartha-Vári, Judith-Hajnal, Moisă, Mădălina Elena, Bencze, László Csaba, Irimie, Florin-Dan, Paizs, Csaba, Toșa, Monica Ioana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037990/
https://www.ncbi.nlm.nih.gov/pubmed/32028723
http://dx.doi.org/10.3390/molecules25030651
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author Bartha-Vári, Judith-Hajnal
Moisă, Mădălina Elena
Bencze, László Csaba
Irimie, Florin-Dan
Paizs, Csaba
Toșa, Monica Ioana
author_facet Bartha-Vári, Judith-Hajnal
Moisă, Mădălina Elena
Bencze, László Csaba
Irimie, Florin-Dan
Paizs, Csaba
Toșa, Monica Ioana
author_sort Bartha-Vári, Judith-Hajnal
collection PubMed
description The Amano lipase from Pseudomonas fluorescens (L-AK) was covalently immobilized on various carbon nanomaterials (functionalized single-walled carbon nanotubes and graphene oxide) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized L-AK on SwCNT(NH2) derivatized with glycerol diglycidyl ether) under optimal conditions, quasi-complete conversion (>99%) of sunflower oil was obtained after only 4 h reaction time. Moreover, the biocatalyst maintained more than 99% of its initial activity in the batch system after multiple recycling experiments.
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spelling pubmed-70379902020-03-10 Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens Bartha-Vári, Judith-Hajnal Moisă, Mădălina Elena Bencze, László Csaba Irimie, Florin-Dan Paizs, Csaba Toșa, Monica Ioana Molecules Article The Amano lipase from Pseudomonas fluorescens (L-AK) was covalently immobilized on various carbon nanomaterials (functionalized single-walled carbon nanotubes and graphene oxide) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized L-AK on SwCNT(NH2) derivatized with glycerol diglycidyl ether) under optimal conditions, quasi-complete conversion (>99%) of sunflower oil was obtained after only 4 h reaction time. Moreover, the biocatalyst maintained more than 99% of its initial activity in the batch system after multiple recycling experiments. MDPI 2020-02-03 /pmc/articles/PMC7037990/ /pubmed/32028723 http://dx.doi.org/10.3390/molecules25030651 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bartha-Vári, Judith-Hajnal
Moisă, Mădălina Elena
Bencze, László Csaba
Irimie, Florin-Dan
Paizs, Csaba
Toșa, Monica Ioana
Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens
title Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens
title_full Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens
title_fullStr Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens
title_full_unstemmed Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens
title_short Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens
title_sort efficient biodiesel production catalyzed by nanobioconjugate of lipase from pseudomonas fluorescens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037990/
https://www.ncbi.nlm.nih.gov/pubmed/32028723
http://dx.doi.org/10.3390/molecules25030651
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