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Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis

OBJECTIVE: To evaluate the possible biological effect of allogenic mesenchymal stem cells (MSCs) combined with tissue fusion technology on the anastomosis. METHODS: Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control...

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Autores principales: Pan, Hong, Lam, Ping Keun, Tong, See W., Leung, Kevin K., Teoh, Anthony Y., Ng, Enders K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038387/
https://www.ncbi.nlm.nih.gov/pubmed/32104185
http://dx.doi.org/10.1155/2020/5142797
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author Pan, Hong
Lam, Ping Keun
Tong, See W.
Leung, Kevin K.
Teoh, Anthony Y.
Ng, Enders K.
author_facet Pan, Hong
Lam, Ping Keun
Tong, See W.
Leung, Kevin K.
Teoh, Anthony Y.
Ng, Enders K.
author_sort Pan, Hong
collection PubMed
description OBJECTIVE: To evaluate the possible biological effect of allogenic mesenchymal stem cells (MSCs) combined with tissue fusion technology on the anastomosis. METHODS: Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control group. Five anastomoses per animal were established using LigaSure ForceTriad (Covidien, MA, USA), a tissue sealing system. Cell migration and tissue-specific differentiation potency, in addition to potential cytokine and genetic changes, were investigated. RESULTS: There were no significant between-group differences in postoperative complications and anastomosis burst pressure. The number of proliferating cell nuclear antigen- (PCNA-) positive cells was significantly higher in the MSC-treated group as compared with that in the control group (P = 0.021). Labeled MSCs were found in the mucosal layer, villus, and lamina propria, as well as in the lamina muscularis mucosae, where they exhibited characteristics of smooth muscle cells. CONCLUSIONS: Grafted MSCs significantly promoted epithelial and connective cell proliferation and maintained their cell migration capacity and differentiation potential in the fused anastomotic tissues, without causing severe postoperative complications.
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spelling pubmed-70383872020-02-26 Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis Pan, Hong Lam, Ping Keun Tong, See W. Leung, Kevin K. Teoh, Anthony Y. Ng, Enders K. Stem Cells Int Research Article OBJECTIVE: To evaluate the possible biological effect of allogenic mesenchymal stem cells (MSCs) combined with tissue fusion technology on the anastomosis. METHODS: Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control group. Five anastomoses per animal were established using LigaSure ForceTriad (Covidien, MA, USA), a tissue sealing system. Cell migration and tissue-specific differentiation potency, in addition to potential cytokine and genetic changes, were investigated. RESULTS: There were no significant between-group differences in postoperative complications and anastomosis burst pressure. The number of proliferating cell nuclear antigen- (PCNA-) positive cells was significantly higher in the MSC-treated group as compared with that in the control group (P = 0.021). Labeled MSCs were found in the mucosal layer, villus, and lamina propria, as well as in the lamina muscularis mucosae, where they exhibited characteristics of smooth muscle cells. CONCLUSIONS: Grafted MSCs significantly promoted epithelial and connective cell proliferation and maintained their cell migration capacity and differentiation potential in the fused anastomotic tissues, without causing severe postoperative complications. Hindawi 2020-02-12 /pmc/articles/PMC7038387/ /pubmed/32104185 http://dx.doi.org/10.1155/2020/5142797 Text en Copyright © 2020 Hong Pan et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pan, Hong
Lam, Ping Keun
Tong, See W.
Leung, Kevin K.
Teoh, Anthony Y.
Ng, Enders K.
Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis
title Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis
title_full Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis
title_fullStr Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis
title_full_unstemmed Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis
title_short Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis
title_sort mesenchymal stem cells combined with tissue fusion technology promoted wound healing in porcine bowel anastomosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038387/
https://www.ncbi.nlm.nih.gov/pubmed/32104185
http://dx.doi.org/10.1155/2020/5142797
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