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Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells

Delineation of factors which affect wound healing would be of immense value to enable on-time or early healing and reduce comorbidities associated with infections or biochemical stress like diabetes. Plasma gelsolin has been identified earlier to significantly enable injury recovery compared to plac...

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Autores principales: Vaid, Bhavna, Chopra, Bhupinder Singh, Raut, Sachin, Sagar, Amin, Badmalia, Maulik D., Ashish, Khatri, Neeraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038438/
https://www.ncbi.nlm.nih.gov/pubmed/32104532
http://dx.doi.org/10.1155/2020/4045365
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author Vaid, Bhavna
Chopra, Bhupinder Singh
Raut, Sachin
Sagar, Amin
Badmalia, Maulik D.
Ashish,
Khatri, Neeraj
author_facet Vaid, Bhavna
Chopra, Bhupinder Singh
Raut, Sachin
Sagar, Amin
Badmalia, Maulik D.
Ashish,
Khatri, Neeraj
author_sort Vaid, Bhavna
collection PubMed
description Delineation of factors which affect wound healing would be of immense value to enable on-time or early healing and reduce comorbidities associated with infections or biochemical stress like diabetes. Plasma gelsolin has been identified earlier to significantly enable injury recovery compared to placebo. This study evaluates the role of rhuGSN for its antioxidant and wound healing properties in murine fibroblasts (3T3-L1 cell line). Total antioxidant capacity of rhuGSN increased in a concentration-dependent manner (0.75-200 μg/mL). Cells pretreated with 0.375 and 0.75 μg/mL rhuGSN for 24 h exhibited a significant increase in viability in a MTT assay. Preincubation of cells with rhuGSN for 24 h followed by oxidative stress induced by exposure to H(2)O(2) for 3 h showed cytoprotective effect. rhuGSN at 12.5 and 25 μg/mL concentration showed an enhanced cell migration after 20 h of injury in a scratch wound healing assay. The proinflammatory cytokine IL-6 levels were elevated in the culture supernatant. These results establish an effective role of rhuGSN against oxidative stress induced by H(2)O(2) and in wound healing of 3T3-L1 fibroblast cells.
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spelling pubmed-70384382020-02-26 Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells Vaid, Bhavna Chopra, Bhupinder Singh Raut, Sachin Sagar, Amin Badmalia, Maulik D. Ashish, Khatri, Neeraj Oxid Med Cell Longev Research Article Delineation of factors which affect wound healing would be of immense value to enable on-time or early healing and reduce comorbidities associated with infections or biochemical stress like diabetes. Plasma gelsolin has been identified earlier to significantly enable injury recovery compared to placebo. This study evaluates the role of rhuGSN for its antioxidant and wound healing properties in murine fibroblasts (3T3-L1 cell line). Total antioxidant capacity of rhuGSN increased in a concentration-dependent manner (0.75-200 μg/mL). Cells pretreated with 0.375 and 0.75 μg/mL rhuGSN for 24 h exhibited a significant increase in viability in a MTT assay. Preincubation of cells with rhuGSN for 24 h followed by oxidative stress induced by exposure to H(2)O(2) for 3 h showed cytoprotective effect. rhuGSN at 12.5 and 25 μg/mL concentration showed an enhanced cell migration after 20 h of injury in a scratch wound healing assay. The proinflammatory cytokine IL-6 levels were elevated in the culture supernatant. These results establish an effective role of rhuGSN against oxidative stress induced by H(2)O(2) and in wound healing of 3T3-L1 fibroblast cells. Hindawi 2020-02-12 /pmc/articles/PMC7038438/ /pubmed/32104532 http://dx.doi.org/10.1155/2020/4045365 Text en Copyright © 2020 Bhavna Vaid et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vaid, Bhavna
Chopra, Bhupinder Singh
Raut, Sachin
Sagar, Amin
Badmalia, Maulik D.
Ashish,
Khatri, Neeraj
Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells
title Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells
title_full Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells
title_fullStr Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells
title_full_unstemmed Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells
title_short Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells
title_sort antioxidant and wound healing property of gelsolin in 3t3-l1 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038438/
https://www.ncbi.nlm.nih.gov/pubmed/32104532
http://dx.doi.org/10.1155/2020/4045365
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