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A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
The amphibian order Caudata, contains several important model species for biological research. However, there is need to generate transcriptome data from representative species of the primary salamander families. Here we describe a de novo reference transcriptome for a terrestrial salamander, Bolito...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038590/ https://www.ncbi.nlm.nih.gov/pubmed/32123704 http://dx.doi.org/10.1016/j.dib.2020.105256 |
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author | Arenas Gómez, Claudia M. Woodcock, M. Ryan Smith, Jeramiah J. Voss, S. Randal Delgado, Jean Paul |
author_facet | Arenas Gómez, Claudia M. Woodcock, M. Ryan Smith, Jeramiah J. Voss, S. Randal Delgado, Jean Paul |
author_sort | Arenas Gómez, Claudia M. |
collection | PubMed |
description | The amphibian order Caudata, contains several important model species for biological research. However, there is need to generate transcriptome data from representative species of the primary salamander families. Here we describe a de novo reference transcriptome for a terrestrial salamander, Bolitoglossa vallecula (Caudata: Plethodontidae). We employed paired-end (PE) illumina RNA sequencing to assemble a de novo reference transcriptome for B. vallecula. Assembled transcripts were compared against sequences from other vertebrate taxa to identify orthologous genes, and compared to the transcriptome of a close plethodontid relative (Bolitoglossa ramosi) to identify commonly expressed genes in the skin. This dataset should be useful to future comparative studies aimed at understanding important biological process, such as immunity, wound healing, and the production of antimicrobial compounds. |
format | Online Article Text |
id | pubmed-7038590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70385902020-03-02 A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia Arenas Gómez, Claudia M. Woodcock, M. Ryan Smith, Jeramiah J. Voss, S. Randal Delgado, Jean Paul Data Brief Biochemistry, Genetics and Molecular Biology The amphibian order Caudata, contains several important model species for biological research. However, there is need to generate transcriptome data from representative species of the primary salamander families. Here we describe a de novo reference transcriptome for a terrestrial salamander, Bolitoglossa vallecula (Caudata: Plethodontidae). We employed paired-end (PE) illumina RNA sequencing to assemble a de novo reference transcriptome for B. vallecula. Assembled transcripts were compared against sequences from other vertebrate taxa to identify orthologous genes, and compared to the transcriptome of a close plethodontid relative (Bolitoglossa ramosi) to identify commonly expressed genes in the skin. This dataset should be useful to future comparative studies aimed at understanding important biological process, such as immunity, wound healing, and the production of antimicrobial compounds. Elsevier 2020-02-11 /pmc/articles/PMC7038590/ /pubmed/32123704 http://dx.doi.org/10.1016/j.dib.2020.105256 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Arenas Gómez, Claudia M. Woodcock, M. Ryan Smith, Jeramiah J. Voss, S. Randal Delgado, Jean Paul A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia |
title | A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia |
title_full | A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia |
title_fullStr | A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia |
title_full_unstemmed | A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia |
title_short | A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia |
title_sort | de novo reference transcriptome for bolitoglossa vallecula, an andean mountain salamander in colombia |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038590/ https://www.ncbi.nlm.nih.gov/pubmed/32123704 http://dx.doi.org/10.1016/j.dib.2020.105256 |
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