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A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia

The amphibian order Caudata, contains several important model species for biological research. However, there is need to generate transcriptome data from representative species of the primary salamander families. Here we describe a de novo reference transcriptome for a terrestrial salamander, Bolito...

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Autores principales: Arenas Gómez, Claudia M., Woodcock, M. Ryan, Smith, Jeramiah J., Voss, S. Randal, Delgado, Jean Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038590/
https://www.ncbi.nlm.nih.gov/pubmed/32123704
http://dx.doi.org/10.1016/j.dib.2020.105256
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author Arenas Gómez, Claudia M.
Woodcock, M. Ryan
Smith, Jeramiah J.
Voss, S. Randal
Delgado, Jean Paul
author_facet Arenas Gómez, Claudia M.
Woodcock, M. Ryan
Smith, Jeramiah J.
Voss, S. Randal
Delgado, Jean Paul
author_sort Arenas Gómez, Claudia M.
collection PubMed
description The amphibian order Caudata, contains several important model species for biological research. However, there is need to generate transcriptome data from representative species of the primary salamander families. Here we describe a de novo reference transcriptome for a terrestrial salamander, Bolitoglossa vallecula (Caudata: Plethodontidae). We employed paired-end (PE) illumina RNA sequencing to assemble a de novo reference transcriptome for B. vallecula. Assembled transcripts were compared against sequences from other vertebrate taxa to identify orthologous genes, and compared to the transcriptome of a close plethodontid relative (Bolitoglossa ramosi) to identify commonly expressed genes in the skin. This dataset should be useful to future comparative studies aimed at understanding important biological process, such as immunity, wound healing, and the production of antimicrobial compounds.
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spelling pubmed-70385902020-03-02 A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia Arenas Gómez, Claudia M. Woodcock, M. Ryan Smith, Jeramiah J. Voss, S. Randal Delgado, Jean Paul Data Brief Biochemistry, Genetics and Molecular Biology The amphibian order Caudata, contains several important model species for biological research. However, there is need to generate transcriptome data from representative species of the primary salamander families. Here we describe a de novo reference transcriptome for a terrestrial salamander, Bolitoglossa vallecula (Caudata: Plethodontidae). We employed paired-end (PE) illumina RNA sequencing to assemble a de novo reference transcriptome for B. vallecula. Assembled transcripts were compared against sequences from other vertebrate taxa to identify orthologous genes, and compared to the transcriptome of a close plethodontid relative (Bolitoglossa ramosi) to identify commonly expressed genes in the skin. This dataset should be useful to future comparative studies aimed at understanding important biological process, such as immunity, wound healing, and the production of antimicrobial compounds. Elsevier 2020-02-11 /pmc/articles/PMC7038590/ /pubmed/32123704 http://dx.doi.org/10.1016/j.dib.2020.105256 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Arenas Gómez, Claudia M.
Woodcock, M. Ryan
Smith, Jeramiah J.
Voss, S. Randal
Delgado, Jean Paul
A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
title A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
title_full A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
title_fullStr A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
title_full_unstemmed A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
title_short A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
title_sort de novo reference transcriptome for bolitoglossa vallecula, an andean mountain salamander in colombia
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038590/
https://www.ncbi.nlm.nih.gov/pubmed/32123704
http://dx.doi.org/10.1016/j.dib.2020.105256
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