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Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs

BACKGROUND: Glycomer 631 and lactomer 9–1 are absorbable suture materials indicated for soft tissue approximation in non-infected wounds. Pigs are often used as surgical models in translational research; however, reports of tissue reactions to both suture materials in pigs are limited. The aim of th...

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Autores principales: Plavec, Tanja, Švara, Tanja, Zdovc, Irena, Gombač, Mitja, Damjanovska, Marija, Stopar Pintarič, Tatjana, Cvetko, Erika, Seliškar, Alenka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038600/
https://www.ncbi.nlm.nih.gov/pubmed/32160877
http://dx.doi.org/10.1186/s12917-020-02290-x
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author Plavec, Tanja
Švara, Tanja
Zdovc, Irena
Gombač, Mitja
Damjanovska, Marija
Stopar Pintarič, Tatjana
Cvetko, Erika
Seliškar, Alenka
author_facet Plavec, Tanja
Švara, Tanja
Zdovc, Irena
Gombač, Mitja
Damjanovska, Marija
Stopar Pintarič, Tatjana
Cvetko, Erika
Seliškar, Alenka
author_sort Plavec, Tanja
collection PubMed
description BACKGROUND: Glycomer 631 and lactomer 9–1 are absorbable suture materials indicated for soft tissue approximation in non-infected wounds. Pigs are often used as surgical models in translational research; however, reports of tissue reactions to both suture materials in pigs are limited. The aim of this study was to assess clinical and histologic responses of skin incisions closed with a subcuticular technique using glycomer 631 and lactomer 9–1 in pigs. RESULTS: Skin incisions on 17 pigs were closed with glycomer 631 and lactomer 9–1, and a clinical reactive score (CRS) including erythema, swelling, discharge and dehiscence was calculated on postoperative days 7 and 14. Subcuticular tissue reaction was assessed histologically on postoperative day 14 (the presence of extravascular neutrophils, macrophages, multinucleated giant cells, lymphocytes, fibroblasts, bacterial colonies and the overall severity of the inflammatory response to the suture material), and the cumulative score of the variables was calculated as an aggregate tissue irritation score (ATIS). Tissue samples were examined for suture extrusion and evaluated microbiologically. The clinical reactive score did not differ between the suture materials. Only one ATIS variable, namely the overall severity of the inflammatory response, was lower (p = 0.029) when glycomer 631 was used. Suture extrusion was found in 10/17 of the incisions closed by glycomer 631 and in 7/13 of the incisions closed by lactomer 9–1. Trueperella pyogenes was isolated from the skin and from the area of tissue reaction in six pigs. CONCLUSIONS: No difference in CRS between the suture materials was observed, and thus both materials may be used for the subcuticular technique in pigs. Glycomer 631 induced less tissue reaction only in terms of the overall severity of the inflammatory response. Suture extrusion was observed in more than 50% of incisions regardless of the suture material, possibly due to a large amount of suture material in the wound. Trueperella pyogenes was the only pathogen isolated from the tissue surrounding the suture material.
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spelling pubmed-70386002020-03-02 Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs Plavec, Tanja Švara, Tanja Zdovc, Irena Gombač, Mitja Damjanovska, Marija Stopar Pintarič, Tatjana Cvetko, Erika Seliškar, Alenka BMC Vet Res Research Article BACKGROUND: Glycomer 631 and lactomer 9–1 are absorbable suture materials indicated for soft tissue approximation in non-infected wounds. Pigs are often used as surgical models in translational research; however, reports of tissue reactions to both suture materials in pigs are limited. The aim of this study was to assess clinical and histologic responses of skin incisions closed with a subcuticular technique using glycomer 631 and lactomer 9–1 in pigs. RESULTS: Skin incisions on 17 pigs were closed with glycomer 631 and lactomer 9–1, and a clinical reactive score (CRS) including erythema, swelling, discharge and dehiscence was calculated on postoperative days 7 and 14. Subcuticular tissue reaction was assessed histologically on postoperative day 14 (the presence of extravascular neutrophils, macrophages, multinucleated giant cells, lymphocytes, fibroblasts, bacterial colonies and the overall severity of the inflammatory response to the suture material), and the cumulative score of the variables was calculated as an aggregate tissue irritation score (ATIS). Tissue samples were examined for suture extrusion and evaluated microbiologically. The clinical reactive score did not differ between the suture materials. Only one ATIS variable, namely the overall severity of the inflammatory response, was lower (p = 0.029) when glycomer 631 was used. Suture extrusion was found in 10/17 of the incisions closed by glycomer 631 and in 7/13 of the incisions closed by lactomer 9–1. Trueperella pyogenes was isolated from the skin and from the area of tissue reaction in six pigs. CONCLUSIONS: No difference in CRS between the suture materials was observed, and thus both materials may be used for the subcuticular technique in pigs. Glycomer 631 induced less tissue reaction only in terms of the overall severity of the inflammatory response. Suture extrusion was observed in more than 50% of incisions regardless of the suture material, possibly due to a large amount of suture material in the wound. Trueperella pyogenes was the only pathogen isolated from the tissue surrounding the suture material. BioMed Central 2020-02-24 /pmc/articles/PMC7038600/ /pubmed/32160877 http://dx.doi.org/10.1186/s12917-020-02290-x Text en © The Author(s) 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Plavec, Tanja
Švara, Tanja
Zdovc, Irena
Gombač, Mitja
Damjanovska, Marija
Stopar Pintarič, Tatjana
Cvetko, Erika
Seliškar, Alenka
Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs
title Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs
title_full Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs
title_fullStr Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs
title_full_unstemmed Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs
title_short Clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs
title_sort clinical and histologic tissue responses of skin incisions closed with glycomer 631 and lactomer 9–1 using a subcuticular technique in pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038600/
https://www.ncbi.nlm.nih.gov/pubmed/32160877
http://dx.doi.org/10.1186/s12917-020-02290-x
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