Cargando…
A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea
General molecular principles of ribosome biogenesis have been well explored in bacteria and eukaryotes. Collectively, these studies have revealed important functional differences and few similarities between these processes. Phylogenetic studies suggest that the information processing machineries fr...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038931/ https://www.ncbi.nlm.nih.gov/pubmed/31828323 http://dx.doi.org/10.1093/nar/gkz1156 |
_version_ | 1783500731297300480 |
---|---|
author | Jüttner, Michael Weiß, Matthias Ostheimer, Nina Reglin, Corinna Kern, Michael Knüppel, Robert Ferreira-Cerca, Sébastien |
author_facet | Jüttner, Michael Weiß, Matthias Ostheimer, Nina Reglin, Corinna Kern, Michael Knüppel, Robert Ferreira-Cerca, Sébastien |
author_sort | Jüttner, Michael |
collection | PubMed |
description | General molecular principles of ribosome biogenesis have been well explored in bacteria and eukaryotes. Collectively, these studies have revealed important functional differences and few similarities between these processes. Phylogenetic studies suggest that the information processing machineries from archaea and eukaryotes are evolutionary more closely related than their bacterial counterparts. These observations raise the question of how ribosome synthesis in archaea may proceed in vivo. In this study, we describe a versatile plasmid-based cis-acting reporter system allowing to analyze in vivo the consequences of ribosomal RNA mutations in the model archaeon Haloferax volcanii. Applying this system, we provide evidence that the bulge-helix-bulge motif enclosed within the ribosomal RNA processing stems is required for the formation of archaeal-specific circular-pre-rRNA intermediates and mature rRNAs. In addition, we have collected evidences suggesting functional coordination of the early steps of ribosome synthesis in H. volcanii. Together our investigation describes a versatile platform allowing to generate and functionally analyze the fate of diverse rRNA variants, thereby paving the way to better understand the cis-acting molecular determinants necessary for archaeal ribosome synthesis, maturation, stability and function. |
format | Online Article Text |
id | pubmed-7038931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70389312020-03-02 A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea Jüttner, Michael Weiß, Matthias Ostheimer, Nina Reglin, Corinna Kern, Michael Knüppel, Robert Ferreira-Cerca, Sébastien Nucleic Acids Res RNA and RNA-protein complexes General molecular principles of ribosome biogenesis have been well explored in bacteria and eukaryotes. Collectively, these studies have revealed important functional differences and few similarities between these processes. Phylogenetic studies suggest that the information processing machineries from archaea and eukaryotes are evolutionary more closely related than their bacterial counterparts. These observations raise the question of how ribosome synthesis in archaea may proceed in vivo. In this study, we describe a versatile plasmid-based cis-acting reporter system allowing to analyze in vivo the consequences of ribosomal RNA mutations in the model archaeon Haloferax volcanii. Applying this system, we provide evidence that the bulge-helix-bulge motif enclosed within the ribosomal RNA processing stems is required for the formation of archaeal-specific circular-pre-rRNA intermediates and mature rRNAs. In addition, we have collected evidences suggesting functional coordination of the early steps of ribosome synthesis in H. volcanii. Together our investigation describes a versatile platform allowing to generate and functionally analyze the fate of diverse rRNA variants, thereby paving the way to better understand the cis-acting molecular determinants necessary for archaeal ribosome synthesis, maturation, stability and function. Oxford University Press 2020-02-28 2019-12-12 /pmc/articles/PMC7038931/ /pubmed/31828323 http://dx.doi.org/10.1093/nar/gkz1156 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA and RNA-protein complexes Jüttner, Michael Weiß, Matthias Ostheimer, Nina Reglin, Corinna Kern, Michael Knüppel, Robert Ferreira-Cerca, Sébastien A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea |
title | A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea |
title_full | A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea |
title_fullStr | A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea |
title_full_unstemmed | A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea |
title_short | A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea |
title_sort | versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal rna mutants in archaea |
topic | RNA and RNA-protein complexes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038931/ https://www.ncbi.nlm.nih.gov/pubmed/31828323 http://dx.doi.org/10.1093/nar/gkz1156 |
work_keys_str_mv | AT juttnermichael aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT weißmatthias aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT ostheimernina aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT reglincorinna aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT kernmichael aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT knuppelrobert aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT ferreiracercasebastien aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT juttnermichael versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT weißmatthias versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT ostheimernina versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT reglincorinna versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT kernmichael versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT knuppelrobert versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea AT ferreiracercasebastien versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea |