Cargando…

A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea

General molecular principles of ribosome biogenesis have been well explored in bacteria and eukaryotes. Collectively, these studies have revealed important functional differences and few similarities between these processes. Phylogenetic studies suggest that the information processing machineries fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Jüttner, Michael, Weiß, Matthias, Ostheimer, Nina, Reglin, Corinna, Kern, Michael, Knüppel, Robert, Ferreira-Cerca, Sébastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038931/
https://www.ncbi.nlm.nih.gov/pubmed/31828323
http://dx.doi.org/10.1093/nar/gkz1156
_version_ 1783500731297300480
author Jüttner, Michael
Weiß, Matthias
Ostheimer, Nina
Reglin, Corinna
Kern, Michael
Knüppel, Robert
Ferreira-Cerca, Sébastien
author_facet Jüttner, Michael
Weiß, Matthias
Ostheimer, Nina
Reglin, Corinna
Kern, Michael
Knüppel, Robert
Ferreira-Cerca, Sébastien
author_sort Jüttner, Michael
collection PubMed
description General molecular principles of ribosome biogenesis have been well explored in bacteria and eukaryotes. Collectively, these studies have revealed important functional differences and few similarities between these processes. Phylogenetic studies suggest that the information processing machineries from archaea and eukaryotes are evolutionary more closely related than their bacterial counterparts. These observations raise the question of how ribosome synthesis in archaea may proceed in vivo. In this study, we describe a versatile plasmid-based cis-acting reporter system allowing to analyze in vivo the consequences of ribosomal RNA mutations in the model archaeon Haloferax volcanii. Applying this system, we provide evidence that the bulge-helix-bulge motif enclosed within the ribosomal RNA processing stems is required for the formation of archaeal-specific circular-pre-rRNA intermediates and mature rRNAs. In addition, we have collected evidences suggesting functional coordination of the early steps of ribosome synthesis in H. volcanii. Together our investigation describes a versatile platform allowing to generate and functionally analyze the fate of diverse rRNA variants, thereby paving the way to better understand the cis-acting molecular determinants necessary for archaeal ribosome synthesis, maturation, stability and function.
format Online
Article
Text
id pubmed-7038931
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-70389312020-03-02 A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea Jüttner, Michael Weiß, Matthias Ostheimer, Nina Reglin, Corinna Kern, Michael Knüppel, Robert Ferreira-Cerca, Sébastien Nucleic Acids Res RNA and RNA-protein complexes General molecular principles of ribosome biogenesis have been well explored in bacteria and eukaryotes. Collectively, these studies have revealed important functional differences and few similarities between these processes. Phylogenetic studies suggest that the information processing machineries from archaea and eukaryotes are evolutionary more closely related than their bacterial counterparts. These observations raise the question of how ribosome synthesis in archaea may proceed in vivo. In this study, we describe a versatile plasmid-based cis-acting reporter system allowing to analyze in vivo the consequences of ribosomal RNA mutations in the model archaeon Haloferax volcanii. Applying this system, we provide evidence that the bulge-helix-bulge motif enclosed within the ribosomal RNA processing stems is required for the formation of archaeal-specific circular-pre-rRNA intermediates and mature rRNAs. In addition, we have collected evidences suggesting functional coordination of the early steps of ribosome synthesis in H. volcanii. Together our investigation describes a versatile platform allowing to generate and functionally analyze the fate of diverse rRNA variants, thereby paving the way to better understand the cis-acting molecular determinants necessary for archaeal ribosome synthesis, maturation, stability and function. Oxford University Press 2020-02-28 2019-12-12 /pmc/articles/PMC7038931/ /pubmed/31828323 http://dx.doi.org/10.1093/nar/gkz1156 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA and RNA-protein complexes
Jüttner, Michael
Weiß, Matthias
Ostheimer, Nina
Reglin, Corinna
Kern, Michael
Knüppel, Robert
Ferreira-Cerca, Sébastien
A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea
title A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea
title_full A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea
title_fullStr A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea
title_full_unstemmed A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea
title_short A versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal RNA mutants in archaea
title_sort versatile cis-acting element reporter system to study the function, maturation and stability of ribosomal rna mutants in archaea
topic RNA and RNA-protein complexes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038931/
https://www.ncbi.nlm.nih.gov/pubmed/31828323
http://dx.doi.org/10.1093/nar/gkz1156
work_keys_str_mv AT juttnermichael aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT weißmatthias aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT ostheimernina aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT reglincorinna aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT kernmichael aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT knuppelrobert aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT ferreiracercasebastien aversatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT juttnermichael versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT weißmatthias versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT ostheimernina versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT reglincorinna versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT kernmichael versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT knuppelrobert versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea
AT ferreiracercasebastien versatilecisactingelementreportersystemtostudythefunctionmaturationandstabilityofribosomalrnamutantsinarchaea