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Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178)
PURPOSE: To investigate the occurrence and genetic characteristics of the bla(IMP-26)-positive plasmid from a multidrug-resistant clinical isolate, Enterobacter hormaechei L51. METHODS: Species identification was determined by MALDI-TOF MS and Sanger sequencing. Antimicrobial susceptibility testing...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039083/ https://www.ncbi.nlm.nih.gov/pubmed/32110070 http://dx.doi.org/10.2147/IDR.S232514 |
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author | Gou, Jian-Jun Liu, Na Guo, Li-Hua Xu, Hao Lv, Tao Yu, Xiao Chen, Yun-Bo Guo, Xiao-Bing Rao, Yu-Ting Zheng, Bei-Wen |
author_facet | Gou, Jian-Jun Liu, Na Guo, Li-Hua Xu, Hao Lv, Tao Yu, Xiao Chen, Yun-Bo Guo, Xiao-Bing Rao, Yu-Ting Zheng, Bei-Wen |
author_sort | Gou, Jian-Jun |
collection | PubMed |
description | PURPOSE: To investigate the occurrence and genetic characteristics of the bla(IMP-26)-positive plasmid from a multidrug-resistant clinical isolate, Enterobacter hormaechei L51. METHODS: Species identification was determined by MALDI-TOF MS and Sanger sequencing. Antimicrobial susceptibility testing was performed by the agar dilution and broth microdilution. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 and PacBio Sequel platforms, and the genome was annotated by the RAST annotation server. The ANI analysis of genomes was performed using OAT. Phylogenetic reconstruction and analyses were performed using the Harvest suite based on the core-genome SNPs of 61 publicly available E. hormaechei genomes. RESULTS: The E. hormaechei L51 genome consists of a 5,018,729 bp circular chromosome and a 343,918 bp conjugative IncHI2/2A plasmid pEHZJ1 encoding bla(IMP-26) which surrounding genetic context was intI1-bla(IMP-26)-ltrA-qacE∆1-sul1. A new sequence type (ST1103) was assigned for the isolate L51 which was resistant to cephalosporins, carbapenems, but sensitive to piperacillin-tazobactam, amikacin, tigecycline, trimethoprim-sulfamethoxazole and colistin. Phylogenetic analysis demonstrated that E. hormaechei L51 belonged to the same subspecies as the reference strain E. hormaechei SCEH020042, however 18,248 divergent SNP were identified. Resistance genes in pEHZJ1 including aac(3)-IIc, aac(6ʹ)-IIc, bla(SHV-178), bla(DHA-1), bla(TEM-1), bla(IMP-26), ereA2, catII, fosA5, qnrB4, tet(D), sul1 and dfrA19. CONCLUSION: In our study, we identified a conjugative IncHI2/2A plasmid carrying bla(IMP-26) and bla(SHV-178) in E. hormaechei ST1103, a novel multidrug-resistant strain isolated from China, and describe the underlying resistance mechanisms of the strain and detailed genetic context of mega plasmid pEHZJ1. |
format | Online Article Text |
id | pubmed-7039083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-70390832020-02-27 Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178) Gou, Jian-Jun Liu, Na Guo, Li-Hua Xu, Hao Lv, Tao Yu, Xiao Chen, Yun-Bo Guo, Xiao-Bing Rao, Yu-Ting Zheng, Bei-Wen Infect Drug Resist Original Research PURPOSE: To investigate the occurrence and genetic characteristics of the bla(IMP-26)-positive plasmid from a multidrug-resistant clinical isolate, Enterobacter hormaechei L51. METHODS: Species identification was determined by MALDI-TOF MS and Sanger sequencing. Antimicrobial susceptibility testing was performed by the agar dilution and broth microdilution. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 and PacBio Sequel platforms, and the genome was annotated by the RAST annotation server. The ANI analysis of genomes was performed using OAT. Phylogenetic reconstruction and analyses were performed using the Harvest suite based on the core-genome SNPs of 61 publicly available E. hormaechei genomes. RESULTS: The E. hormaechei L51 genome consists of a 5,018,729 bp circular chromosome and a 343,918 bp conjugative IncHI2/2A plasmid pEHZJ1 encoding bla(IMP-26) which surrounding genetic context was intI1-bla(IMP-26)-ltrA-qacE∆1-sul1. A new sequence type (ST1103) was assigned for the isolate L51 which was resistant to cephalosporins, carbapenems, but sensitive to piperacillin-tazobactam, amikacin, tigecycline, trimethoprim-sulfamethoxazole and colistin. Phylogenetic analysis demonstrated that E. hormaechei L51 belonged to the same subspecies as the reference strain E. hormaechei SCEH020042, however 18,248 divergent SNP were identified. Resistance genes in pEHZJ1 including aac(3)-IIc, aac(6ʹ)-IIc, bla(SHV-178), bla(DHA-1), bla(TEM-1), bla(IMP-26), ereA2, catII, fosA5, qnrB4, tet(D), sul1 and dfrA19. CONCLUSION: In our study, we identified a conjugative IncHI2/2A plasmid carrying bla(IMP-26) and bla(SHV-178) in E. hormaechei ST1103, a novel multidrug-resistant strain isolated from China, and describe the underlying resistance mechanisms of the strain and detailed genetic context of mega plasmid pEHZJ1. Dove 2020-02-20 /pmc/articles/PMC7039083/ /pubmed/32110070 http://dx.doi.org/10.2147/IDR.S232514 Text en © 2020 Gou et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Gou, Jian-Jun Liu, Na Guo, Li-Hua Xu, Hao Lv, Tao Yu, Xiao Chen, Yun-Bo Guo, Xiao-Bing Rao, Yu-Ting Zheng, Bei-Wen Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178) |
title | Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178) |
title_full | Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178) |
title_fullStr | Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178) |
title_full_unstemmed | Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178) |
title_short | Carbapenem-Resistant Enterobacter hormaechei ST1103 with IMP-26 Carbapenemase and ESBL Gene bla(SHV-178) |
title_sort | carbapenem-resistant enterobacter hormaechei st1103 with imp-26 carbapenemase and esbl gene bla(shv-178) |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039083/ https://www.ncbi.nlm.nih.gov/pubmed/32110070 http://dx.doi.org/10.2147/IDR.S232514 |
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