Cargando…
Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma
Intrahepatic cholangiocarcinoma (iCCA) is a highly malignant neoplasm arising from the intrahepatic bile ducts. As a scaffold protein of lipid rafts, flotillin-2 is upregulated in several types of cancer and promotes tumor progression and metastasis. To the best of our knowledge, the present study w...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039164/ https://www.ncbi.nlm.nih.gov/pubmed/32194722 http://dx.doi.org/10.3892/ol.2020.11349 |
_version_ | 1783500772495851520 |
---|---|
author | Xu, Zhiying Wang, Tao Song, Haiyang Jiang, Xuewen |
author_facet | Xu, Zhiying Wang, Tao Song, Haiyang Jiang, Xuewen |
author_sort | Xu, Zhiying |
collection | PubMed |
description | Intrahepatic cholangiocarcinoma (iCCA) is a highly malignant neoplasm arising from the intrahepatic bile ducts. As a scaffold protein of lipid rafts, flotillin-2 is upregulated in several types of cancer and promotes tumor progression and metastasis. To the best of our knowledge, the present study was the first to detect the upregulation of flotillin-2 in iCCA tissues compared with matched adjacent non-tumor tissues. In addition, immunohistochemistry was used to investigate the expression of flotillin-2 in a microarray consisting of 92 iCCA tissues. A total of 59 samples (64.1%) exhibited high flotillin-2 expression, which was significantly related to lymph node metastasis (P=0.029) and tumor-node-metastasis stage (P=0.016). Further in vitro study demonstrated that knockdown of flotillin-2 inhibited the invasive capability of iCCA cell lines, further supporting the participation of flotillin-2 in cancer invasion and metastasis. Moreover, Kaplan-Meier analysis showed patients with high flotillin-2 expression had worse overall survival outcomes. The multivariate Cox proportional hazards model further revealed that high flotillin-2 expression was an independent indicator (P=0.005) of poor prognosis for patients with iCCA. Collectively, the present study revealed that as a promoter of invasion and an independent marker of poor prognosis, flotillin-2 may serve as a potential target for the development of novel therapeutic agents for iCCA. |
format | Online Article Text |
id | pubmed-7039164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-70391642020-03-19 Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma Xu, Zhiying Wang, Tao Song, Haiyang Jiang, Xuewen Oncol Lett Articles Intrahepatic cholangiocarcinoma (iCCA) is a highly malignant neoplasm arising from the intrahepatic bile ducts. As a scaffold protein of lipid rafts, flotillin-2 is upregulated in several types of cancer and promotes tumor progression and metastasis. To the best of our knowledge, the present study was the first to detect the upregulation of flotillin-2 in iCCA tissues compared with matched adjacent non-tumor tissues. In addition, immunohistochemistry was used to investigate the expression of flotillin-2 in a microarray consisting of 92 iCCA tissues. A total of 59 samples (64.1%) exhibited high flotillin-2 expression, which was significantly related to lymph node metastasis (P=0.029) and tumor-node-metastasis stage (P=0.016). Further in vitro study demonstrated that knockdown of flotillin-2 inhibited the invasive capability of iCCA cell lines, further supporting the participation of flotillin-2 in cancer invasion and metastasis. Moreover, Kaplan-Meier analysis showed patients with high flotillin-2 expression had worse overall survival outcomes. The multivariate Cox proportional hazards model further revealed that high flotillin-2 expression was an independent indicator (P=0.005) of poor prognosis for patients with iCCA. Collectively, the present study revealed that as a promoter of invasion and an independent marker of poor prognosis, flotillin-2 may serve as a potential target for the development of novel therapeutic agents for iCCA. D.A. Spandidos 2020-03 2020-01-24 /pmc/articles/PMC7039164/ /pubmed/32194722 http://dx.doi.org/10.3892/ol.2020.11349 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Xu, Zhiying Wang, Tao Song, Haiyang Jiang, Xuewen Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma |
title | Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma |
title_full | Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma |
title_fullStr | Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma |
title_full_unstemmed | Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma |
title_short | Flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma |
title_sort | flotillin-2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039164/ https://www.ncbi.nlm.nih.gov/pubmed/32194722 http://dx.doi.org/10.3892/ol.2020.11349 |
work_keys_str_mv | AT xuzhiying flotillin2predictspoorprognosisandpromotestumorinvasioninintrahepaticcholangiocarcinoma AT wangtao flotillin2predictspoorprognosisandpromotestumorinvasioninintrahepaticcholangiocarcinoma AT songhaiyang flotillin2predictspoorprognosisandpromotestumorinvasioninintrahepaticcholangiocarcinoma AT jiangxuewen flotillin2predictspoorprognosisandpromotestumorinvasioninintrahepaticcholangiocarcinoma |