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Coupling APEX labeling to imaging mass spectrometry of single organelles reveals heterogeneity in lysosomal protein turnover

Quantification of stable isotope tracers after metabolic labeling provides a snapshot of the dynamic state of living cells and tissue. A form of imaging mass spectrometry quantifies isotope ratios with a lateral resolution <50 nm, using a methodology that we refer to as multi-isotope imaging mass...

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Detalles Bibliográficos
Autores principales:	Narendra, Derek P., Guillermier, Christelle, Gyngard, Frank, Huang, Xiaoping, Ward, Michael E., Steinhauser, Matthew L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Rockefeller University Press 2019
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039203/ https://www.ncbi.nlm.nih.gov/pubmed/31719114 http://dx.doi.org/10.1083/jcb.201901097

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