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Ciliary force-responsive striated fibers promote basal body connections and cortical interactions
Multi-ciliary arrays promote fluid flow and cellular motility using the polarized and coordinated beating of hundreds of motile cilia. Tetrahymena basal bodies (BBs) nucleate and position cilia, whereby BB-associated striated fibers (SFs) promote BB anchorage and orientation into ciliary rows. Mutan...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039215/ https://www.ncbi.nlm.nih.gov/pubmed/31740506 http://dx.doi.org/10.1083/jcb.201904091 |
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author | Soh, Adam W.J. van Dam, Teunis J.P. Stemm-Wolf, Alexander J. Pham, Andrew T. Morgan, Garry P. O’Toole, Eileen T. Pearson, Chad G. |
author_facet | Soh, Adam W.J. van Dam, Teunis J.P. Stemm-Wolf, Alexander J. Pham, Andrew T. Morgan, Garry P. O’Toole, Eileen T. Pearson, Chad G. |
author_sort | Soh, Adam W.J. |
collection | PubMed |
description | Multi-ciliary arrays promote fluid flow and cellular motility using the polarized and coordinated beating of hundreds of motile cilia. Tetrahymena basal bodies (BBs) nucleate and position cilia, whereby BB-associated striated fibers (SFs) promote BB anchorage and orientation into ciliary rows. Mutants that shorten SFs cause disoriented BBs. In contrast to the cytotaxis model, we show that disoriented BBs with short SFs can regain normal orientation if SF length is restored. In addition, SFs adopt unique lengths by their shrinkage and growth to establish and maintain BB connections and cortical interactions in a ciliary force-dependent mechanism. Tetrahymena SFs comprise at least eight uniquely localizing proteins belonging to the SF-assemblin family. Loss of different proteins that localize to the SF base disrupts either SF steady-state length or ciliary force-induced SF elongation. Thus, the dynamic regulation of SFs promotes BB connections and cortical interactions to organize ciliary arrays. |
format | Online Article Text |
id | pubmed-7039215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70392152020-07-06 Ciliary force-responsive striated fibers promote basal body connections and cortical interactions Soh, Adam W.J. van Dam, Teunis J.P. Stemm-Wolf, Alexander J. Pham, Andrew T. Morgan, Garry P. O’Toole, Eileen T. Pearson, Chad G. J Cell Biol Research Articles Multi-ciliary arrays promote fluid flow and cellular motility using the polarized and coordinated beating of hundreds of motile cilia. Tetrahymena basal bodies (BBs) nucleate and position cilia, whereby BB-associated striated fibers (SFs) promote BB anchorage and orientation into ciliary rows. Mutants that shorten SFs cause disoriented BBs. In contrast to the cytotaxis model, we show that disoriented BBs with short SFs can regain normal orientation if SF length is restored. In addition, SFs adopt unique lengths by their shrinkage and growth to establish and maintain BB connections and cortical interactions in a ciliary force-dependent mechanism. Tetrahymena SFs comprise at least eight uniquely localizing proteins belonging to the SF-assemblin family. Loss of different proteins that localize to the SF base disrupts either SF steady-state length or ciliary force-induced SF elongation. Thus, the dynamic regulation of SFs promotes BB connections and cortical interactions to organize ciliary arrays. Rockefeller University Press 2019-11-18 /pmc/articles/PMC7039215/ /pubmed/31740506 http://dx.doi.org/10.1083/jcb.201904091 Text en © 2019 Soh et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Soh, Adam W.J. van Dam, Teunis J.P. Stemm-Wolf, Alexander J. Pham, Andrew T. Morgan, Garry P. O’Toole, Eileen T. Pearson, Chad G. Ciliary force-responsive striated fibers promote basal body connections and cortical interactions |
title | Ciliary force-responsive striated fibers promote basal body connections and cortical interactions |
title_full | Ciliary force-responsive striated fibers promote basal body connections and cortical interactions |
title_fullStr | Ciliary force-responsive striated fibers promote basal body connections and cortical interactions |
title_full_unstemmed | Ciliary force-responsive striated fibers promote basal body connections and cortical interactions |
title_short | Ciliary force-responsive striated fibers promote basal body connections and cortical interactions |
title_sort | ciliary force-responsive striated fibers promote basal body connections and cortical interactions |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039215/ https://www.ncbi.nlm.nih.gov/pubmed/31740506 http://dx.doi.org/10.1083/jcb.201904091 |
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